Cargando…

Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium

Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by “hair”-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of the actin-filled stereocili...

Descripción completa

Detalles Bibliográficos
Autores principales: Erickson, Timothy, Biggers, William Paul, Williams, Kevin, Butland, Shyanne E., Venuto, Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299642/
https://www.ncbi.nlm.nih.gov/pubmed/37367482
http://dx.doi.org/10.3390/jdb11020028
_version_ 1785064414445043712
author Erickson, Timothy
Biggers, William Paul
Williams, Kevin
Butland, Shyanne E.
Venuto, Alexandra
author_facet Erickson, Timothy
Biggers, William Paul
Williams, Kevin
Butland, Shyanne E.
Venuto, Alexandra
author_sort Erickson, Timothy
collection PubMed
description Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by “hair”-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of the actin-filled stereocilia, the hair bundle features a single, non-motile, true cilium called the kinocilium. The kinocilium plays an important role in bundle development and the mechanics of sensory detection. To understand more about kinocilial development and structure, we performed a transcriptomic analysis of zebrafish hair cells to identify cilia-associated genes that have yet to be characterized in hair cells. In this study, we focused on three such genes—ankef1a, odf3l2a, and saxo2—because human or mouse orthologs are either associated with sensorineural hearing loss or are located near uncharacterized deafness loci. We made transgenic fish that express fluorescently tagged versions of their proteins, demonstrating their localization to the kinocilia of zebrafish hair cells. Furthermore, we found that Ankef1a, Odf3l2a, and Saxo2 exhibit distinct localization patterns along the length of the kinocilium and within the cell body. Lastly, we have reported a novel overexpression phenotype of Saxo2. Overall, these results suggest that the hair cell kinocilium in zebrafish is regionalized along its proximal-distal axis and set the groundwork to understand more about the roles of these kinocilial proteins in hair cells.
format Online
Article
Text
id pubmed-10299642
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-102996422023-06-28 Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium Erickson, Timothy Biggers, William Paul Williams, Kevin Butland, Shyanne E. Venuto, Alexandra J Dev Biol Article Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by “hair”-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of the actin-filled stereocilia, the hair bundle features a single, non-motile, true cilium called the kinocilium. The kinocilium plays an important role in bundle development and the mechanics of sensory detection. To understand more about kinocilial development and structure, we performed a transcriptomic analysis of zebrafish hair cells to identify cilia-associated genes that have yet to be characterized in hair cells. In this study, we focused on three such genes—ankef1a, odf3l2a, and saxo2—because human or mouse orthologs are either associated with sensorineural hearing loss or are located near uncharacterized deafness loci. We made transgenic fish that express fluorescently tagged versions of their proteins, demonstrating their localization to the kinocilia of zebrafish hair cells. Furthermore, we found that Ankef1a, Odf3l2a, and Saxo2 exhibit distinct localization patterns along the length of the kinocilium and within the cell body. Lastly, we have reported a novel overexpression phenotype of Saxo2. Overall, these results suggest that the hair cell kinocilium in zebrafish is regionalized along its proximal-distal axis and set the groundwork to understand more about the roles of these kinocilial proteins in hair cells. MDPI 2023-06-16 /pmc/articles/PMC10299642/ /pubmed/37367482 http://dx.doi.org/10.3390/jdb11020028 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Erickson, Timothy
Biggers, William Paul
Williams, Kevin
Butland, Shyanne E.
Venuto, Alexandra
Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
title Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
title_full Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
title_fullStr Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
title_full_unstemmed Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
title_short Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
title_sort regionalized protein localization domains in the zebrafish hair cell kinocilium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299642/
https://www.ncbi.nlm.nih.gov/pubmed/37367482
http://dx.doi.org/10.3390/jdb11020028
work_keys_str_mv AT ericksontimothy regionalizedproteinlocalizationdomainsinthezebrafishhaircellkinocilium
AT biggerswilliampaul regionalizedproteinlocalizationdomainsinthezebrafishhaircellkinocilium
AT williamskevin regionalizedproteinlocalizationdomainsinthezebrafishhaircellkinocilium
AT butlandshyannee regionalizedproteinlocalizationdomainsinthezebrafishhaircellkinocilium
AT venutoalexandra regionalizedproteinlocalizationdomainsinthezebrafishhaircellkinocilium