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Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium
Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by “hair”-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of the actin-filled stereocili...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299642/ https://www.ncbi.nlm.nih.gov/pubmed/37367482 http://dx.doi.org/10.3390/jdb11020028 |
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author | Erickson, Timothy Biggers, William Paul Williams, Kevin Butland, Shyanne E. Venuto, Alexandra |
author_facet | Erickson, Timothy Biggers, William Paul Williams, Kevin Butland, Shyanne E. Venuto, Alexandra |
author_sort | Erickson, Timothy |
collection | PubMed |
description | Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by “hair”-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of the actin-filled stereocilia, the hair bundle features a single, non-motile, true cilium called the kinocilium. The kinocilium plays an important role in bundle development and the mechanics of sensory detection. To understand more about kinocilial development and structure, we performed a transcriptomic analysis of zebrafish hair cells to identify cilia-associated genes that have yet to be characterized in hair cells. In this study, we focused on three such genes—ankef1a, odf3l2a, and saxo2—because human or mouse orthologs are either associated with sensorineural hearing loss or are located near uncharacterized deafness loci. We made transgenic fish that express fluorescently tagged versions of their proteins, demonstrating their localization to the kinocilia of zebrafish hair cells. Furthermore, we found that Ankef1a, Odf3l2a, and Saxo2 exhibit distinct localization patterns along the length of the kinocilium and within the cell body. Lastly, we have reported a novel overexpression phenotype of Saxo2. Overall, these results suggest that the hair cell kinocilium in zebrafish is regionalized along its proximal-distal axis and set the groundwork to understand more about the roles of these kinocilial proteins in hair cells. |
format | Online Article Text |
id | pubmed-10299642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-102996422023-06-28 Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium Erickson, Timothy Biggers, William Paul Williams, Kevin Butland, Shyanne E. Venuto, Alexandra J Dev Biol Article Sensory hair cells are the receptors for auditory, vestibular, and lateral line sensory organs in vertebrates. These cells are distinguished by “hair”-like projections from their apical surface collectively known as the hair bundle. Along with the staircase arrangement of the actin-filled stereocilia, the hair bundle features a single, non-motile, true cilium called the kinocilium. The kinocilium plays an important role in bundle development and the mechanics of sensory detection. To understand more about kinocilial development and structure, we performed a transcriptomic analysis of zebrafish hair cells to identify cilia-associated genes that have yet to be characterized in hair cells. In this study, we focused on three such genes—ankef1a, odf3l2a, and saxo2—because human or mouse orthologs are either associated with sensorineural hearing loss or are located near uncharacterized deafness loci. We made transgenic fish that express fluorescently tagged versions of their proteins, demonstrating their localization to the kinocilia of zebrafish hair cells. Furthermore, we found that Ankef1a, Odf3l2a, and Saxo2 exhibit distinct localization patterns along the length of the kinocilium and within the cell body. Lastly, we have reported a novel overexpression phenotype of Saxo2. Overall, these results suggest that the hair cell kinocilium in zebrafish is regionalized along its proximal-distal axis and set the groundwork to understand more about the roles of these kinocilial proteins in hair cells. MDPI 2023-06-16 /pmc/articles/PMC10299642/ /pubmed/37367482 http://dx.doi.org/10.3390/jdb11020028 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Erickson, Timothy Biggers, William Paul Williams, Kevin Butland, Shyanne E. Venuto, Alexandra Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium |
title | Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium |
title_full | Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium |
title_fullStr | Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium |
title_full_unstemmed | Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium |
title_short | Regionalized Protein Localization Domains in the Zebrafish Hair Cell Kinocilium |
title_sort | regionalized protein localization domains in the zebrafish hair cell kinocilium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299642/ https://www.ncbi.nlm.nih.gov/pubmed/37367482 http://dx.doi.org/10.3390/jdb11020028 |
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