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Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping

Despite claims of safety or harm reduction for electronic cigarettes (E-cig) use (also known as vaping), emerging evidence indicates that E-cigs are not likely safe, or necessarily safer than traditional cigarettes, when considering the user’s risk of developing vascular dysfunction/disease. E-cigs...

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Detalles Bibliográficos
Autores principales: Pitzer, Christopher R., Aboaziza, Eiman A., O’Reilly, Juliana M., Mandler, W. Kyle, Olfert, I. Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299710/
https://www.ncbi.nlm.nih.gov/pubmed/37373356
http://dx.doi.org/10.3390/ijms241210208
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author Pitzer, Christopher R.
Aboaziza, Eiman A.
O’Reilly, Juliana M.
Mandler, W. Kyle
Olfert, I. Mark
author_facet Pitzer, Christopher R.
Aboaziza, Eiman A.
O’Reilly, Juliana M.
Mandler, W. Kyle
Olfert, I. Mark
author_sort Pitzer, Christopher R.
collection PubMed
description Despite claims of safety or harm reduction for electronic cigarettes (E-cig) use (also known as vaping), emerging evidence indicates that E-cigs are not likely safe, or necessarily safer than traditional cigarettes, when considering the user’s risk of developing vascular dysfunction/disease. E-cigs are different from regular cigarettes in that E-cig devices are highly customizable, and users can change the e-liquid composition (such as the base solution, flavors, and nicotine level). Since the effects of E-cigs on the microvascular responses in skeletal muscle are poorly understood, we used intravital microscopy with an acute (one-time 10 puff) exposure paradigm to evaluate the individual components of e-liquid on vascular tone and endothelial function in the arterioles of the gluteus maximus muscle of anesthetized C57Bl/6 mice. Consistent with the molecular responses seen with endothelial cells, we found that the peripheral vasoconstriction response was similar between mice exposed to E-cig aerosol or cigarette smoke (i.e., 3R4F reference cigarette); this response was not nicotine dependent, and endothelial cell-mediated vasodilation was not altered within this acute exposure paradigm. We also report that, regardless of the base solution component [i.e., vegetable glycerin (VG)-only or propylene glycol (PG)-only], the vasoconstriction responses were the same in mice with inhalation exposure to 3R4F cigarette smoke or E-cig aerosol. Key findings from this work reveal that some component other than nicotine, in inhaled smoke or aerosol, is responsible for triggering peripheral vasoconstriction in skeletal muscle, and that regardless of one’s preference for an E-cig base solution composition (i.e., ratio of VG-to-PG), the acute physiological response to blood vessels appears to be the same. The data suggest that vaping is not likely to be ‘safer’ than smoking towards blood vessels and can be expected to produce and/or result in the same adverse vascular health outcomes associated with smoking cigarettes.
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spelling pubmed-102997102023-06-28 Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping Pitzer, Christopher R. Aboaziza, Eiman A. O’Reilly, Juliana M. Mandler, W. Kyle Olfert, I. Mark Int J Mol Sci Communication Despite claims of safety or harm reduction for electronic cigarettes (E-cig) use (also known as vaping), emerging evidence indicates that E-cigs are not likely safe, or necessarily safer than traditional cigarettes, when considering the user’s risk of developing vascular dysfunction/disease. E-cigs are different from regular cigarettes in that E-cig devices are highly customizable, and users can change the e-liquid composition (such as the base solution, flavors, and nicotine level). Since the effects of E-cigs on the microvascular responses in skeletal muscle are poorly understood, we used intravital microscopy with an acute (one-time 10 puff) exposure paradigm to evaluate the individual components of e-liquid on vascular tone and endothelial function in the arterioles of the gluteus maximus muscle of anesthetized C57Bl/6 mice. Consistent with the molecular responses seen with endothelial cells, we found that the peripheral vasoconstriction response was similar between mice exposed to E-cig aerosol or cigarette smoke (i.e., 3R4F reference cigarette); this response was not nicotine dependent, and endothelial cell-mediated vasodilation was not altered within this acute exposure paradigm. We also report that, regardless of the base solution component [i.e., vegetable glycerin (VG)-only or propylene glycol (PG)-only], the vasoconstriction responses were the same in mice with inhalation exposure to 3R4F cigarette smoke or E-cig aerosol. Key findings from this work reveal that some component other than nicotine, in inhaled smoke or aerosol, is responsible for triggering peripheral vasoconstriction in skeletal muscle, and that regardless of one’s preference for an E-cig base solution composition (i.e., ratio of VG-to-PG), the acute physiological response to blood vessels appears to be the same. The data suggest that vaping is not likely to be ‘safer’ than smoking towards blood vessels and can be expected to produce and/or result in the same adverse vascular health outcomes associated with smoking cigarettes. MDPI 2023-06-16 /pmc/articles/PMC10299710/ /pubmed/37373356 http://dx.doi.org/10.3390/ijms241210208 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Pitzer, Christopher R.
Aboaziza, Eiman A.
O’Reilly, Juliana M.
Mandler, W. Kyle
Olfert, I. Mark
Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping
title Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping
title_full Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping
title_fullStr Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping
title_full_unstemmed Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping
title_short Nicotine and Microvascular Responses in Skeletal Muscle from Acute Exposure to Cigarettes and Vaping
title_sort nicotine and microvascular responses in skeletal muscle from acute exposure to cigarettes and vaping
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10299710/
https://www.ncbi.nlm.nih.gov/pubmed/37373356
http://dx.doi.org/10.3390/ijms241210208
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