A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions

West Nile virus (WNV) causes skin lesions in farmed crocodiles leading to the depreciation of the value of their hides and significant economic losses. However, there is no commercially available vaccine designed for use in crocodilians against WNV. We tested chimeric virus vaccines composed of the...

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Autores principales: Habarugira, Gervais, Harrison, Jessica J., Moran, Jasmin, Suen, Willy W., Colmant, Agathe M. G., Hobson-Peters, Jody, Isberg, Sally R., Bielefeldt-Ohmann, Helle, Hall, Roy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300036/
https://www.ncbi.nlm.nih.gov/pubmed/37369653
http://dx.doi.org/10.1038/s41541-023-00688-w
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author Habarugira, Gervais
Harrison, Jessica J.
Moran, Jasmin
Suen, Willy W.
Colmant, Agathe M. G.
Hobson-Peters, Jody
Isberg, Sally R.
Bielefeldt-Ohmann, Helle
Hall, Roy A.
author_facet Habarugira, Gervais
Harrison, Jessica J.
Moran, Jasmin
Suen, Willy W.
Colmant, Agathe M. G.
Hobson-Peters, Jody
Isberg, Sally R.
Bielefeldt-Ohmann, Helle
Hall, Roy A.
author_sort Habarugira, Gervais
collection PubMed
description West Nile virus (WNV) causes skin lesions in farmed crocodiles leading to the depreciation of the value of their hides and significant economic losses. However, there is no commercially available vaccine designed for use in crocodilians against WNV. We tested chimeric virus vaccines composed of the non-structural genes of the insect-specific flavivirus Binjari virus (BinJV) and genes encoding the structural proteins of WNV. The BinJV/WNV chimera, is antigenically similar to wild-type WNV but replication-defective in vertebrates. Intramuscular injection of two doses of BinJV/WNV in hatchling saltwater crocodiles (Crocodylus porosus) elicited a robust neutralising antibody response and conferred protection against viremia and skin lesions after challenge with WNV. In contrast, mock-vaccinated crocodiles became viraemic and 22.2% exhibited WNV-induced lesions. This suggests that the BinJV/WNV chimera is a safe and efficacious vaccine for preventing WNV-induced skin lesions in farmed crocodilians.
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spelling pubmed-103000362023-06-29 A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions Habarugira, Gervais Harrison, Jessica J. Moran, Jasmin Suen, Willy W. Colmant, Agathe M. G. Hobson-Peters, Jody Isberg, Sally R. Bielefeldt-Ohmann, Helle Hall, Roy A. NPJ Vaccines Article West Nile virus (WNV) causes skin lesions in farmed crocodiles leading to the depreciation of the value of their hides and significant economic losses. However, there is no commercially available vaccine designed for use in crocodilians against WNV. We tested chimeric virus vaccines composed of the non-structural genes of the insect-specific flavivirus Binjari virus (BinJV) and genes encoding the structural proteins of WNV. The BinJV/WNV chimera, is antigenically similar to wild-type WNV but replication-defective in vertebrates. Intramuscular injection of two doses of BinJV/WNV in hatchling saltwater crocodiles (Crocodylus porosus) elicited a robust neutralising antibody response and conferred protection against viremia and skin lesions after challenge with WNV. In contrast, mock-vaccinated crocodiles became viraemic and 22.2% exhibited WNV-induced lesions. This suggests that the BinJV/WNV chimera is a safe and efficacious vaccine for preventing WNV-induced skin lesions in farmed crocodilians. Nature Publishing Group UK 2023-06-27 /pmc/articles/PMC10300036/ /pubmed/37369653 http://dx.doi.org/10.1038/s41541-023-00688-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Habarugira, Gervais
Harrison, Jessica J.
Moran, Jasmin
Suen, Willy W.
Colmant, Agathe M. G.
Hobson-Peters, Jody
Isberg, Sally R.
Bielefeldt-Ohmann, Helle
Hall, Roy A.
A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions
title A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions
title_full A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions
title_fullStr A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions
title_full_unstemmed A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions
title_short A chimeric vaccine protects farmed saltwater crocodiles from West Nile virus-induced skin lesions
title_sort chimeric vaccine protects farmed saltwater crocodiles from west nile virus-induced skin lesions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300036/
https://www.ncbi.nlm.nih.gov/pubmed/37369653
http://dx.doi.org/10.1038/s41541-023-00688-w
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