An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism

We herein elucidate the function of SARS-CoV-2derived 5'UTR in the human cells. 5'UTR bound host cellular RNAs were immunoprecipitated by gRNA-dCas13 (targeting luciferase RNA fused to SARS-CoV-2 5'UTR) in HEK293T and A549 cells. The 5'UTR bound RNA extractions were predominantly...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimizu, Yurika, Bandaru, Srinivas, Hara, Mari, Young, Sonny, Sano, Toshikazu, Usami, Kaya, Kurano, Yuta, Lee, Suni, Kumagai-Takei, Naoko, Takashiba, Shogo, Sano, Shunji, Ito, Tatsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300121/
https://www.ncbi.nlm.nih.gov/pubmed/37369697
http://dx.doi.org/10.1038/s41598-023-36680-6
_version_ 1785064518802472960
author Shimizu, Yurika
Bandaru, Srinivas
Hara, Mari
Young, Sonny
Sano, Toshikazu
Usami, Kaya
Kurano, Yuta
Lee, Suni
Kumagai-Takei, Naoko
Takashiba, Shogo
Sano, Shunji
Ito, Tatsuo
author_facet Shimizu, Yurika
Bandaru, Srinivas
Hara, Mari
Young, Sonny
Sano, Toshikazu
Usami, Kaya
Kurano, Yuta
Lee, Suni
Kumagai-Takei, Naoko
Takashiba, Shogo
Sano, Shunji
Ito, Tatsuo
author_sort Shimizu, Yurika
collection PubMed
description We herein elucidate the function of SARS-CoV-2derived 5'UTR in the human cells. 5'UTR bound host cellular RNAs were immunoprecipitated by gRNA-dCas13 (targeting luciferase RNA fused to SARS-CoV-2 5'UTR) in HEK293T and A549 cells. The 5'UTR bound RNA extractions were predominantly enriched for regulating lipid metabolism. Overexpression of SARS-CoV-2 5'UTR RNA altered the expression of factors involved in the process of the human Mevalonate pathway. In addition, we found that HMG-CoA reductase inhibitors were shown to suppress SARS-CoV-2 5'UTR-mediated translation activities. In conclusion, we deduce the array of host RNAs interacting with SARS-CoV-2 5'UTR that drives SARS-CoV-2 translation and influences host metabolic pathways.
format Online
Article
Text
id pubmed-10300121
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-103001212023-06-29 An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism Shimizu, Yurika Bandaru, Srinivas Hara, Mari Young, Sonny Sano, Toshikazu Usami, Kaya Kurano, Yuta Lee, Suni Kumagai-Takei, Naoko Takashiba, Shogo Sano, Shunji Ito, Tatsuo Sci Rep Article We herein elucidate the function of SARS-CoV-2derived 5'UTR in the human cells. 5'UTR bound host cellular RNAs were immunoprecipitated by gRNA-dCas13 (targeting luciferase RNA fused to SARS-CoV-2 5'UTR) in HEK293T and A549 cells. The 5'UTR bound RNA extractions were predominantly enriched for regulating lipid metabolism. Overexpression of SARS-CoV-2 5'UTR RNA altered the expression of factors involved in the process of the human Mevalonate pathway. In addition, we found that HMG-CoA reductase inhibitors were shown to suppress SARS-CoV-2 5'UTR-mediated translation activities. In conclusion, we deduce the array of host RNAs interacting with SARS-CoV-2 5'UTR that drives SARS-CoV-2 translation and influences host metabolic pathways. Nature Publishing Group UK 2023-06-27 /pmc/articles/PMC10300121/ /pubmed/37369697 http://dx.doi.org/10.1038/s41598-023-36680-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Shimizu, Yurika
Bandaru, Srinivas
Hara, Mari
Young, Sonny
Sano, Toshikazu
Usami, Kaya
Kurano, Yuta
Lee, Suni
Kumagai-Takei, Naoko
Takashiba, Shogo
Sano, Shunji
Ito, Tatsuo
An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism
title An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism
title_full An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism
title_fullStr An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism
title_full_unstemmed An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism
title_short An RNA-immunoprecipitation via CRISPR/dCas13 reveals an interaction between the SARS-CoV-2 5'UTR RNA and the process of human lipid metabolism
title_sort rna-immunoprecipitation via crispr/dcas13 reveals an interaction between the sars-cov-2 5'utr rna and the process of human lipid metabolism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300121/
https://www.ncbi.nlm.nih.gov/pubmed/37369697
http://dx.doi.org/10.1038/s41598-023-36680-6
work_keys_str_mv AT shimizuyurika anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT bandarusrinivas anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT haramari anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT youngsonny anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT sanotoshikazu anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT usamikaya anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT kuranoyuta anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT leesuni anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT kumagaitakeinaoko anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT takashibashogo anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT sanoshunji anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT itotatsuo anrnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT shimizuyurika rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT bandarusrinivas rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT haramari rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT youngsonny rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT sanotoshikazu rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT usamikaya rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT kuranoyuta rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT leesuni rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT kumagaitakeinaoko rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT takashibashogo rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT sanoshunji rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism
AT itotatsuo rnaimmunoprecipitationviacrisprdcas13revealsaninteractionbetweenthesarscov25utrrnaandtheprocessofhumanlipidmetabolism

Ejemplares similares