Cargando…

Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran

STATEMENT OF THE PROBLEM: Determining the prevalence of respiratory viruses' coinfection with coronavirus disease 2019 (COVID-19) is essential to defining its true clinical influence. PURPOSE: This study aimed to evaluate co-infection rates between severe acute respiratory syndrome–related coro...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghapanchi, Jannan, Dehghani Naghvani, Ali, Rezazadeh, Fahimeh, Farzin, Mitra, Moatari, Afagh, Masoudi, Sina, Kalantari, Mohamadreza, Derafshi, Reza, Sedarat, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University of Medical Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300138/
https://www.ncbi.nlm.nih.gov/pubmed/37388206
http://dx.doi.org/10.30476/DENTJODS.2022.92797.1679
_version_ 1785064522803838976
author Ghapanchi, Jannan
Dehghani Naghvani, Ali
Rezazadeh, Fahimeh
Farzin, Mitra
Moatari, Afagh
Masoudi, Sina
Kalantari, Mohamadreza
Derafshi, Reza
Sedarat, Hossein
author_facet Ghapanchi, Jannan
Dehghani Naghvani, Ali
Rezazadeh, Fahimeh
Farzin, Mitra
Moatari, Afagh
Masoudi, Sina
Kalantari, Mohamadreza
Derafshi, Reza
Sedarat, Hossein
author_sort Ghapanchi, Jannan
collection PubMed
description STATEMENT OF THE PROBLEM: Determining the prevalence of respiratory viruses' coinfection with coronavirus disease 2019 (COVID-19) is essential to defining its true clinical influence. PURPOSE: This study aimed to evaluate co-infection rates between severe acute respiratory syndrome–related coronavirus 2 (SARS-CoV-2) and respiratory syncytial virus (RSV) in infected patients in Shiraz, south of Iran. MATERIALS AND METHOD: In a cross-sectional descriptive study, oropharyngeal, nasopharyngeal aspirate (NPA), and saliva samples of 50 COVID-19 patients who were referred to Ali-Asghar hospital (Shiraz, Iran) from March to August 2020, were collected. A control group consisted of age and sex-matched healthy participants. The nasopharyngeal and oropharyngeal aspirates were collected by sterile swabs. All cases were hospitalized, and all SARS-CoV-2 patients had a fever and respiratory symptoms. The samples were packed in a vial with 1 mL of transport medium and transported to the Valfagre specialty laboratory, where they were tested for RSV using a real-time polymerase chain reaction (PCR). RESULTS: 100 nasopharyngeal/oropharyngeal aspirates and saliva samples including 50 healthy controls (24 females, 26 males) and 50 COVID-19 patients' samples (27 males and 23 females) were studied. There was no significant difference regarding age as well as gender between both groups (p> 0.05). None of the healthy subjects was infected with RSV; however, 5(10%) patients from COVID-19 group were infected with the RSV virus. Chi-square test did not show a significant difference between RSV infection in COVID-19 patients and healthy subjects. CONCLUSION: The outcome of present research showed that concurrent RSV with COVID 19 infection might be seen in hospitalized patients in Shiraz Southwest of Iran. For more reliable findings, further research on bigger populations, including more pathogens in several places around the country, and considering the severity of symptoms is required.
format Online
Article
Text
id pubmed-10300138
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Shiraz University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-103001382023-06-29 Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran Ghapanchi, Jannan Dehghani Naghvani, Ali Rezazadeh, Fahimeh Farzin, Mitra Moatari, Afagh Masoudi, Sina Kalantari, Mohamadreza Derafshi, Reza Sedarat, Hossein J Dent (Shiraz) Original Article STATEMENT OF THE PROBLEM: Determining the prevalence of respiratory viruses' coinfection with coronavirus disease 2019 (COVID-19) is essential to defining its true clinical influence. PURPOSE: This study aimed to evaluate co-infection rates between severe acute respiratory syndrome–related coronavirus 2 (SARS-CoV-2) and respiratory syncytial virus (RSV) in infected patients in Shiraz, south of Iran. MATERIALS AND METHOD: In a cross-sectional descriptive study, oropharyngeal, nasopharyngeal aspirate (NPA), and saliva samples of 50 COVID-19 patients who were referred to Ali-Asghar hospital (Shiraz, Iran) from March to August 2020, were collected. A control group consisted of age and sex-matched healthy participants. The nasopharyngeal and oropharyngeal aspirates were collected by sterile swabs. All cases were hospitalized, and all SARS-CoV-2 patients had a fever and respiratory symptoms. The samples were packed in a vial with 1 mL of transport medium and transported to the Valfagre specialty laboratory, where they were tested for RSV using a real-time polymerase chain reaction (PCR). RESULTS: 100 nasopharyngeal/oropharyngeal aspirates and saliva samples including 50 healthy controls (24 females, 26 males) and 50 COVID-19 patients' samples (27 males and 23 females) were studied. There was no significant difference regarding age as well as gender between both groups (p> 0.05). None of the healthy subjects was infected with RSV; however, 5(10%) patients from COVID-19 group were infected with the RSV virus. Chi-square test did not show a significant difference between RSV infection in COVID-19 patients and healthy subjects. CONCLUSION: The outcome of present research showed that concurrent RSV with COVID 19 infection might be seen in hospitalized patients in Shiraz Southwest of Iran. For more reliable findings, further research on bigger populations, including more pathogens in several places around the country, and considering the severity of symptoms is required. Shiraz University of Medical Sciences 2023-06 /pmc/articles/PMC10300138/ /pubmed/37388206 http://dx.doi.org/10.30476/DENTJODS.2022.92797.1679 Text en Copyright: © Journal of Dentistry https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License, ( http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits reusers to copy and redistribute the material in any medium or format if the original work is properly cited, and attribution is given to the creator. The license also permits for commercial use.
spellingShingle Original Article
Ghapanchi, Jannan
Dehghani Naghvani, Ali
Rezazadeh, Fahimeh
Farzin, Mitra
Moatari, Afagh
Masoudi, Sina
Kalantari, Mohamadreza
Derafshi, Reza
Sedarat, Hossein
Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran
title Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran
title_full Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran
title_fullStr Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran
title_full_unstemmed Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran
title_short Co-Infection Rates between SARS-CoV-2 and RSV in Oropharyngeal, Nasopharyngeal Aspirate and Saliva Samples of COVID-19 Patients, Shiraz, South of Iran
title_sort co-infection rates between sars-cov-2 and rsv in oropharyngeal, nasopharyngeal aspirate and saliva samples of covid-19 patients, shiraz, south of iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300138/
https://www.ncbi.nlm.nih.gov/pubmed/37388206
http://dx.doi.org/10.30476/DENTJODS.2022.92797.1679
work_keys_str_mv AT ghapanchijannan coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT dehghaninaghvaniali coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT rezazadehfahimeh coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT farzinmitra coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT moatariafagh coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT masoudisina coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT kalantarimohamadreza coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT derafshireza coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran
AT sedarathossein coinfectionratesbetweensarscov2andrsvinoropharyngealnasopharyngealaspirateandsalivasamplesofcovid19patientsshirazsouthofiran