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Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum

There is evidence that RNA editing is related to plant cellular stress as well as electron transport organelles, such as mitochondria. The mitochondrial atp1 gene encodes the alpha-subunit of Atp synthase. Control as well as two periods of drought stress treatments were analyzed in the cDNAs generat...

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Autores principales: Ibrahim, Mona I.M., Ramadan, Ahmed M., Amer, Marwa, Khan, Thana K., Mohamed, Nermin G., Said, Osama A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300253/
https://www.ncbi.nlm.nih.gov/pubmed/37389198
http://dx.doi.org/10.1016/j.sjbs.2023.103703
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author Ibrahim, Mona I.M.
Ramadan, Ahmed M.
Amer, Marwa
Khan, Thana K.
Mohamed, Nermin G.
Said, Osama A.
author_facet Ibrahim, Mona I.M.
Ramadan, Ahmed M.
Amer, Marwa
Khan, Thana K.
Mohamed, Nermin G.
Said, Osama A.
author_sort Ibrahim, Mona I.M.
collection PubMed
description There is evidence that RNA editing is related to plant cellular stress as well as electron transport organelles, such as mitochondria. The mitochondrial atp1 gene encodes the alpha-subunit of Atp synthase. Control as well as two periods of drought stress treatments were analyzed in the cDNAs generated from the mitochondrial atp1 gene of two cultivars of Triticum aestivum [Giza 168 (G168) and Gemmiza 10 (GM10)]. Following RNA-seq data assembly, atp1 cDNAs from the control (acc. no. OQ129415), 2-hour (acc. no. OQ129416), and 12-hour (acc. no. OQ129417) time points of the T. aestivum cultivar G168 were obtained. Control (acc. no. OQ129419), 2-hour (acc. no. OQ129420), and 12-hour (acc. no. OQ129421) samples all included reconstructed atp1 transcripts from Gemmiza 10. Atp1 transcripts were assembled using the wheat atp1 gene (acc. no. NC_036024). RNA-seq raw data was utilized to identify 11 RNA editing sites in atp1 in the tolerant cultivar Giza168 and 6 in the sensitive cultivar Gemmiza10. The significant difference in RNA editing observed between control and drought stress conditions in sites led to synonymous amino acids. This led to no change in tertiary structure between tolerant and sensitive cultivars. But the change was focused between produced protein and its correspondence sequence on DNA.
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spelling pubmed-103002532023-06-29 Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum Ibrahim, Mona I.M. Ramadan, Ahmed M. Amer, Marwa Khan, Thana K. Mohamed, Nermin G. Said, Osama A. Saudi J Biol Sci Original Article There is evidence that RNA editing is related to plant cellular stress as well as electron transport organelles, such as mitochondria. The mitochondrial atp1 gene encodes the alpha-subunit of Atp synthase. Control as well as two periods of drought stress treatments were analyzed in the cDNAs generated from the mitochondrial atp1 gene of two cultivars of Triticum aestivum [Giza 168 (G168) and Gemmiza 10 (GM10)]. Following RNA-seq data assembly, atp1 cDNAs from the control (acc. no. OQ129415), 2-hour (acc. no. OQ129416), and 12-hour (acc. no. OQ129417) time points of the T. aestivum cultivar G168 were obtained. Control (acc. no. OQ129419), 2-hour (acc. no. OQ129420), and 12-hour (acc. no. OQ129421) samples all included reconstructed atp1 transcripts from Gemmiza 10. Atp1 transcripts were assembled using the wheat atp1 gene (acc. no. NC_036024). RNA-seq raw data was utilized to identify 11 RNA editing sites in atp1 in the tolerant cultivar Giza168 and 6 in the sensitive cultivar Gemmiza10. The significant difference in RNA editing observed between control and drought stress conditions in sites led to synonymous amino acids. This led to no change in tertiary structure between tolerant and sensitive cultivars. But the change was focused between produced protein and its correspondence sequence on DNA. Elsevier 2023-07 2023-06-09 /pmc/articles/PMC10300253/ /pubmed/37389198 http://dx.doi.org/10.1016/j.sjbs.2023.103703 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Ibrahim, Mona I.M.
Ramadan, Ahmed M.
Amer, Marwa
Khan, Thana K.
Mohamed, Nermin G.
Said, Osama A.
Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum
title Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum
title_full Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum
title_fullStr Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum
title_full_unstemmed Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum
title_short Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in Triticum aestivum
title_sort deciphering the enigma of rna editing in the atp1_alpha subunit of atp synthase in triticum aestivum
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300253/
https://www.ncbi.nlm.nih.gov/pubmed/37389198
http://dx.doi.org/10.1016/j.sjbs.2023.103703
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