Cargando…

Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs

Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodon, Jordi, Te, Nigeer, Segalés, Joaquim, Vergara-Alert, Júlia, Bensaid, Albert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300347/
https://www.ncbi.nlm.nih.gov/pubmed/37388723
http://dx.doi.org/10.3389/fimmu.2023.1205080
_version_ 1785064568080302080
author Rodon, Jordi
Te, Nigeer
Segalés, Joaquim
Vergara-Alert, Júlia
Bensaid, Albert
author_facet Rodon, Jordi
Te, Nigeer
Segalés, Joaquim
Vergara-Alert, Júlia
Bensaid, Albert
author_sort Rodon, Jordi
collection PubMed
description Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cells from MERS-CoV convalescent llamas and pulsed them with two different viral strains (clades B and C). Viral replication was not supported in LN, but a cellular immune response was mounted. Reminiscent Th1 responses (IFN-γ, IL-2, IL-12) were elicited upon MERS-CoV sensing, accompanied by a marked and transient peak of antiviral responses (type I IFNs, IFN-λ3, ISGs, PRRs and TFs). Importantly, expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) or inflammasome components (NLRP3, CASP1, PYCARD) was dampened. The role of IFN-λ3 to counterbalance inflammatory processes and bridge innate and adaptive immune responses in camelid species is discussed. Our findings shed light into key mechanisms on how reservoir species control MERS-CoV in the absence of clinical disease.
format Online
Article
Text
id pubmed-10300347
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-103003472023-06-29 Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs Rodon, Jordi Te, Nigeer Segalés, Joaquim Vergara-Alert, Júlia Bensaid, Albert Front Immunol Immunology Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cells from MERS-CoV convalescent llamas and pulsed them with two different viral strains (clades B and C). Viral replication was not supported in LN, but a cellular immune response was mounted. Reminiscent Th1 responses (IFN-γ, IL-2, IL-12) were elicited upon MERS-CoV sensing, accompanied by a marked and transient peak of antiviral responses (type I IFNs, IFN-λ3, ISGs, PRRs and TFs). Importantly, expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) or inflammasome components (NLRP3, CASP1, PYCARD) was dampened. The role of IFN-λ3 to counterbalance inflammatory processes and bridge innate and adaptive immune responses in camelid species is discussed. Our findings shed light into key mechanisms on how reservoir species control MERS-CoV in the absence of clinical disease. Frontiers Media S.A. 2023-06-14 /pmc/articles/PMC10300347/ /pubmed/37388723 http://dx.doi.org/10.3389/fimmu.2023.1205080 Text en Copyright © 2023 Rodon, Te, Segalés, Vergara-Alert and Bensaid https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Rodon, Jordi
Te, Nigeer
Segalés, Joaquim
Vergara-Alert, Júlia
Bensaid, Albert
Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
title Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
title_full Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
title_fullStr Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
title_full_unstemmed Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
title_short Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
title_sort enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon mers-cov sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300347/
https://www.ncbi.nlm.nih.gov/pubmed/37388723
http://dx.doi.org/10.3389/fimmu.2023.1205080
work_keys_str_mv AT rodonjordi enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs
AT tenigeer enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs
AT segalesjoaquim enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs
AT vergaraalertjulia enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs
AT bensaidalbert enhancedantiviralimmunityanddampenedinflammationinllamalymphnodesuponmerscovsensingbridginginnateandadaptivecellularimmuneresponsesincamelidreservoirs