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Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19

OBJECTIVE: SARS-CoV-2 infection has been shown to result in increased circulating levels of adenosine triphosphate and adenosine diphosphate and decreased levels of adenosine, which has important anti-inflammatory activity. The goal of this pilot project was to assess the levels of soluble CD73 and...

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Autores principales: Rud, Jonathan, Riker, Richard R, Eldridge, Ashley, Lord, Christine, deKay, Joanne T, May, Teresa L, Gagnon, David J, Sawyer, Douglas, Ryzhov, Sergey, Seder, David B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300631/
https://www.ncbi.nlm.nih.gov/pubmed/37364162
http://dx.doi.org/10.1177/03946320231185703
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author Rud, Jonathan
Riker, Richard R
Eldridge, Ashley
Lord, Christine
deKay, Joanne T
May, Teresa L
Gagnon, David J
Sawyer, Douglas
Ryzhov, Sergey
Seder, David B
author_facet Rud, Jonathan
Riker, Richard R
Eldridge, Ashley
Lord, Christine
deKay, Joanne T
May, Teresa L
Gagnon, David J
Sawyer, Douglas
Ryzhov, Sergey
Seder, David B
author_sort Rud, Jonathan
collection PubMed
description OBJECTIVE: SARS-CoV-2 infection has been shown to result in increased circulating levels of adenosine triphosphate and adenosine diphosphate and decreased levels of adenosine, which has important anti-inflammatory activity. The goal of this pilot project was to assess the levels of soluble CD73 and soluble Adenosine Deaminase (ADA) in hospitalized patients with COVID-19 and determine if levels of these molecules are associated with disease severity. METHODS: Plasma from 28 PCR-confirmed hospitalized COVID-19 patients who had varied disease severity based on WHO classification (6 mild/moderate, 10 severe, 12 critical) had concentrations of both soluble CD73 and ADA determined by ELISA. These concentrations were compared to healthy control plasma that is commercially available and was biobanked prior to the start of the pandemic. Additionally, outcomes such as WHO ordinal scale for disease severity, ICU admission, needed for invasive ventilation, hospital length of stay, and development of thrombosis during admission were used as markers of disease severity. RESULTS: Our results show that both CD73 and ADA are decreased during SARS-CoV-2 infection. The level of circulating CD73 is directly correlated to the severity of the disease defined by the need for ICU admission, invasive ventilation, and hospital length of stay. Low level of CD73 is also associated with clinical thrombosis, a severe complication of SARS-CoV-2 infection. CONCLUSION: Our study indicates that adenosine metabolism is down-regulated in patients with COVID-19 and associated with severe infection. Further large-scale studies are warranted to investigate the role of the adenosinergic anti-inflammatory CD73/ADA axis in protection against COVID-19.
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spelling pubmed-103006312023-06-29 Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19 Rud, Jonathan Riker, Richard R Eldridge, Ashley Lord, Christine deKay, Joanne T May, Teresa L Gagnon, David J Sawyer, Douglas Ryzhov, Sergey Seder, David B Int J Immunopathol Pharmacol Original Research Article OBJECTIVE: SARS-CoV-2 infection has been shown to result in increased circulating levels of adenosine triphosphate and adenosine diphosphate and decreased levels of adenosine, which has important anti-inflammatory activity. The goal of this pilot project was to assess the levels of soluble CD73 and soluble Adenosine Deaminase (ADA) in hospitalized patients with COVID-19 and determine if levels of these molecules are associated with disease severity. METHODS: Plasma from 28 PCR-confirmed hospitalized COVID-19 patients who had varied disease severity based on WHO classification (6 mild/moderate, 10 severe, 12 critical) had concentrations of both soluble CD73 and ADA determined by ELISA. These concentrations were compared to healthy control plasma that is commercially available and was biobanked prior to the start of the pandemic. Additionally, outcomes such as WHO ordinal scale for disease severity, ICU admission, needed for invasive ventilation, hospital length of stay, and development of thrombosis during admission were used as markers of disease severity. RESULTS: Our results show that both CD73 and ADA are decreased during SARS-CoV-2 infection. The level of circulating CD73 is directly correlated to the severity of the disease defined by the need for ICU admission, invasive ventilation, and hospital length of stay. Low level of CD73 is also associated with clinical thrombosis, a severe complication of SARS-CoV-2 infection. CONCLUSION: Our study indicates that adenosine metabolism is down-regulated in patients with COVID-19 and associated with severe infection. Further large-scale studies are warranted to investigate the role of the adenosinergic anti-inflammatory CD73/ADA axis in protection against COVID-19. SAGE Publications 2023-06-26 /pmc/articles/PMC10300631/ /pubmed/37364162 http://dx.doi.org/10.1177/03946320231185703 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research Article
Rud, Jonathan
Riker, Richard R
Eldridge, Ashley
Lord, Christine
deKay, Joanne T
May, Teresa L
Gagnon, David J
Sawyer, Douglas
Ryzhov, Sergey
Seder, David B
Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19
title Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19
title_full Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19
title_fullStr Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19
title_full_unstemmed Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19
title_short Decreased circulating CD73 and adenosine deaminase are associated with disease severity in hospitalized patients with COVID-19
title_sort decreased circulating cd73 and adenosine deaminase are associated with disease severity in hospitalized patients with covid-19
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300631/
https://www.ncbi.nlm.nih.gov/pubmed/37364162
http://dx.doi.org/10.1177/03946320231185703
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