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Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity

The genus Scrophularia is one of the largest genera belonging to the Scrophulariaceae family. Different members of the genus exhibit an interesting, wide spectrum of bioactivities. Accordingly, the current study aimed to investigate, for the first time, the chemical composition of the essential oil...

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Autores principales: Al-Dalahmeh, Yousef, Almahmoud, Sondos Abdullah J., Al-Bataineh, Nezar, Alghzawi, Taqwa A., Alhamzani, Abdulrahman G., Al-Mutairi, Aamal A., Al-Jaber, Hala I., Abu Orabi, Sultan T., Bataineh, Tareq T., Al-Sheraideh, Mohammed S., Al-Qudah, Mahmoud A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300737/
https://www.ncbi.nlm.nih.gov/pubmed/37374186
http://dx.doi.org/10.3390/life13061404
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author Al-Dalahmeh, Yousef
Almahmoud, Sondos Abdullah J.
Al-Bataineh, Nezar
Alghzawi, Taqwa A.
Alhamzani, Abdulrahman G.
Al-Mutairi, Aamal A.
Al-Jaber, Hala I.
Abu Orabi, Sultan T.
Bataineh, Tareq T.
Al-Sheraideh, Mohammed S.
Al-Qudah, Mahmoud A.
author_facet Al-Dalahmeh, Yousef
Almahmoud, Sondos Abdullah J.
Al-Bataineh, Nezar
Alghzawi, Taqwa A.
Alhamzani, Abdulrahman G.
Al-Mutairi, Aamal A.
Al-Jaber, Hala I.
Abu Orabi, Sultan T.
Bataineh, Tareq T.
Al-Sheraideh, Mohammed S.
Al-Qudah, Mahmoud A.
author_sort Al-Dalahmeh, Yousef
collection PubMed
description The genus Scrophularia is one of the largest genera belonging to the Scrophulariaceae family. Different members of the genus exhibit an interesting, wide spectrum of bioactivities. Accordingly, the current study aimed to investigate, for the first time, the chemical composition of the essential oil of Scrophularia peyronii Post. from Jordan. Additionally, extracts obtained from the aerial parts with solvents of different polarities were assayed for their phytochemical constituents and in vitro antioxidant activities. The major constituents detected in the essential oil, as revealed by GC/MS analysis, contained mainly Z,Z-farnesyl acetone (11.04%), β-elemene (6.36%), n-octanal (5.98%), and spathulenol (4.58%). Each of the aqueous methanol (Sp-M) and butanol (Sp-B) extracts contained flavonoids, saponins, anthraquinone, and glycosides. Both extracts were evaluated for their total phenolic content (TPC), total flavonoid content (TFC), and their in vitro antioxidant activity, which were assayed using the DPPH radical scavenging activity and ABTS radical scavenging methods. Additionally, the two extracts were then subjected to LC-ESI-MS/MS for the qualitative determination of their secondary metabolite content, especially in flavonoids and phenolic compounds. The results showed that the Sp-B extract of S. peyronii had the highest contents of both phenolic compounds and flavonoids and showed high radical scavenging activity, as determined by the two assay methods, when compared with the Sp-M extract. The LC-ESI-MS/MS analysis resulted in the detection of 21 compounds, including 8 flavonoids, 6 phenolic acids, 6 iridoids, and 2 acids. Although the majority of compounds were detected in both extracts, it was noticed that scropolioside B, 6′-O-cinnamoylharpagide, isoferulic acid, and 6-O-methylcatapol were only detected in the Sp-M fraction.
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spelling pubmed-103007372023-06-29 Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity Al-Dalahmeh, Yousef Almahmoud, Sondos Abdullah J. Al-Bataineh, Nezar Alghzawi, Taqwa A. Alhamzani, Abdulrahman G. Al-Mutairi, Aamal A. Al-Jaber, Hala I. Abu Orabi, Sultan T. Bataineh, Tareq T. Al-Sheraideh, Mohammed S. Al-Qudah, Mahmoud A. Life (Basel) Article The genus Scrophularia is one of the largest genera belonging to the Scrophulariaceae family. Different members of the genus exhibit an interesting, wide spectrum of bioactivities. Accordingly, the current study aimed to investigate, for the first time, the chemical composition of the essential oil of Scrophularia peyronii Post. from Jordan. Additionally, extracts obtained from the aerial parts with solvents of different polarities were assayed for their phytochemical constituents and in vitro antioxidant activities. The major constituents detected in the essential oil, as revealed by GC/MS analysis, contained mainly Z,Z-farnesyl acetone (11.04%), β-elemene (6.36%), n-octanal (5.98%), and spathulenol (4.58%). Each of the aqueous methanol (Sp-M) and butanol (Sp-B) extracts contained flavonoids, saponins, anthraquinone, and glycosides. Both extracts were evaluated for their total phenolic content (TPC), total flavonoid content (TFC), and their in vitro antioxidant activity, which were assayed using the DPPH radical scavenging activity and ABTS radical scavenging methods. Additionally, the two extracts were then subjected to LC-ESI-MS/MS for the qualitative determination of their secondary metabolite content, especially in flavonoids and phenolic compounds. The results showed that the Sp-B extract of S. peyronii had the highest contents of both phenolic compounds and flavonoids and showed high radical scavenging activity, as determined by the two assay methods, when compared with the Sp-M extract. The LC-ESI-MS/MS analysis resulted in the detection of 21 compounds, including 8 flavonoids, 6 phenolic acids, 6 iridoids, and 2 acids. Although the majority of compounds were detected in both extracts, it was noticed that scropolioside B, 6′-O-cinnamoylharpagide, isoferulic acid, and 6-O-methylcatapol were only detected in the Sp-M fraction. MDPI 2023-06-16 /pmc/articles/PMC10300737/ /pubmed/37374186 http://dx.doi.org/10.3390/life13061404 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al-Dalahmeh, Yousef
Almahmoud, Sondos Abdullah J.
Al-Bataineh, Nezar
Alghzawi, Taqwa A.
Alhamzani, Abdulrahman G.
Al-Mutairi, Aamal A.
Al-Jaber, Hala I.
Abu Orabi, Sultan T.
Bataineh, Tareq T.
Al-Sheraideh, Mohammed S.
Al-Qudah, Mahmoud A.
Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity
title Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity
title_full Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity
title_fullStr Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity
title_full_unstemmed Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity
title_short Scrophularia peyronii Post. from Jordan: Chemical Composition of Essential Oil and Phytochemical Profiling of Crude Extracts and Their In Vitro Antioxidant Activity
title_sort scrophularia peyronii post. from jordan: chemical composition of essential oil and phytochemical profiling of crude extracts and their in vitro antioxidant activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10300737/
https://www.ncbi.nlm.nih.gov/pubmed/37374186
http://dx.doi.org/10.3390/life13061404
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