Cargando…
Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures
The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approa...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301036/ https://www.ncbi.nlm.nih.gov/pubmed/37375162 http://dx.doi.org/10.3390/molecules28124607 |
_version_ | 1785064717442613248 |
---|---|
author | Sardar, Tauqeer Maqbool, Mehwish Ishtiaq, Muhammad Mazhar, Muhammad Waqas El-Sheikh, Mohamed A. Casini, Ryan Mahmoud, Eman A. Elansary, Hosam O. |
author_facet | Sardar, Tauqeer Maqbool, Mehwish Ishtiaq, Muhammad Mazhar, Muhammad Waqas El-Sheikh, Mohamed A. Casini, Ryan Mahmoud, Eman A. Elansary, Hosam O. |
author_sort | Sardar, Tauqeer |
collection | PubMed |
description | The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approaches and innovative strategies are prevalently applied to optimize phytoconstituents’ production, enhance yield, biomass, ensure sustainable consistency and scalability. In this study, we investigated the effects of elicitation with yeast extract and calcium oxide nanoparticles (CaONPs) on in vitro cultures of Swertia chirata (Roxb. ex Fleming) Karsten. Specifically, we examined the effects of different concentrations of CaONPs in combination with different concentrations of yeast extract on various parameters related to callus growth, antioxidant activity, biomass and phytochemical contents. Our results showed that elicitation with yeast extract and CaONPs had significant effects on the growth and characteristics of callus cultures of S. chirata. The treatments involving yeast extract and CaONPs were found to be the most effective in increasing the contents of total flavonoid contents (TFC), total phenolic contents (TPC), amarogentin and mangiferin. These treatments also led to an improvement in the contents of total anthocyanin and alpha tocopherols. Additionally, the DPPH scavenging activity was significantly increased in the treated samples. Furthermore, the treatments involving elicitation with yeast extract and CaONPs also led to significant improvements in callus growth and characteristics. These treatments promoted callus response from an average to an excellent level and improved the color and nature of the callus from yellow to yellow-brown and greenish and from fragile to compact, respectively. The best response was observed in treatments involving 0.20 g/L yeast extract and 90 ug/L CaONPs. Overall, our findings suggest that elicitation with yeast extract and CaONPs can be a useful strategy for promoting the growth, biomass, phytochemical contents and antioxidant activity of callus cultures of S. chirata in comparison to wild plant herbal drug samples. |
format | Online Article Text |
id | pubmed-10301036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103010362023-06-29 Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures Sardar, Tauqeer Maqbool, Mehwish Ishtiaq, Muhammad Mazhar, Muhammad Waqas El-Sheikh, Mohamed A. Casini, Ryan Mahmoud, Eman A. Elansary, Hosam O. Molecules Article The challenges in the production of metabolites of medicinal potential from wild plants include low yields, slow growth rates, seasonal variations, genetic variability and regulatory as well as ethical constraints. Overcoming these challenges is of paramount significance and interdisciplinary approaches and innovative strategies are prevalently applied to optimize phytoconstituents’ production, enhance yield, biomass, ensure sustainable consistency and scalability. In this study, we investigated the effects of elicitation with yeast extract and calcium oxide nanoparticles (CaONPs) on in vitro cultures of Swertia chirata (Roxb. ex Fleming) Karsten. Specifically, we examined the effects of different concentrations of CaONPs in combination with different concentrations of yeast extract on various parameters related to callus growth, antioxidant activity, biomass and phytochemical contents. Our results showed that elicitation with yeast extract and CaONPs had significant effects on the growth and characteristics of callus cultures of S. chirata. The treatments involving yeast extract and CaONPs were found to be the most effective in increasing the contents of total flavonoid contents (TFC), total phenolic contents (TPC), amarogentin and mangiferin. These treatments also led to an improvement in the contents of total anthocyanin and alpha tocopherols. Additionally, the DPPH scavenging activity was significantly increased in the treated samples. Furthermore, the treatments involving elicitation with yeast extract and CaONPs also led to significant improvements in callus growth and characteristics. These treatments promoted callus response from an average to an excellent level and improved the color and nature of the callus from yellow to yellow-brown and greenish and from fragile to compact, respectively. The best response was observed in treatments involving 0.20 g/L yeast extract and 90 ug/L CaONPs. Overall, our findings suggest that elicitation with yeast extract and CaONPs can be a useful strategy for promoting the growth, biomass, phytochemical contents and antioxidant activity of callus cultures of S. chirata in comparison to wild plant herbal drug samples. MDPI 2023-06-07 /pmc/articles/PMC10301036/ /pubmed/37375162 http://dx.doi.org/10.3390/molecules28124607 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sardar, Tauqeer Maqbool, Mehwish Ishtiaq, Muhammad Mazhar, Muhammad Waqas El-Sheikh, Mohamed A. Casini, Ryan Mahmoud, Eman A. Elansary, Hosam O. Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures |
title | Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures |
title_full | Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures |
title_fullStr | Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures |
title_full_unstemmed | Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures |
title_short | Synergistic Influence of Yeast Extract and Calcium Oxide Nanoparticles on the Synthesis of Bioactive Antioxidants and Metabolites in Swertia chirata In Vitro Callus Cultures |
title_sort | synergistic influence of yeast extract and calcium oxide nanoparticles on the synthesis of bioactive antioxidants and metabolites in swertia chirata in vitro callus cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301036/ https://www.ncbi.nlm.nih.gov/pubmed/37375162 http://dx.doi.org/10.3390/molecules28124607 |
work_keys_str_mv | AT sardartauqeer synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT maqboolmehwish synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT ishtiaqmuhammad synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT mazharmuhammadwaqas synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT elsheikhmohameda synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT casiniryan synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT mahmoudemana synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures AT elansaryhosamo synergisticinfluenceofyeastextractandcalciumoxidenanoparticlesonthesynthesisofbioactiveantioxidantsandmetabolitesinswertiachiratainvitrocalluscultures |