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Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction
Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301044/ https://www.ncbi.nlm.nih.gov/pubmed/37367667 http://dx.doi.org/10.3390/md21060342 |
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author | Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S. Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann |
author_facet | Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S. Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann |
author_sort | Auger, Audrey |
collection | PubMed |
description | Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of N-glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by O-methyl groups. In particular, we show that the N-glycans isolated from Crassostrea gigas and Ostrea edulis exhibit exquisite methylation patterns in their terminal N-acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. |
format | Online Article Text |
id | pubmed-10301044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103010442023-06-29 Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S. Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann Mar Drugs Article Noroviruses, the major cause of acute viral gastroenteritis, are known to bind to histo-blood group antigens (HBGAs), including ABH groups and Lewis-type epitopes, which decorate the surface of erythrocytes and epithelial cells of their host tissues. The biosynthesis of these antigens is controlled by several glycosyltransferases, the distribution and expression of which varies between tissues and individuals. The use of HBGAs as ligands by viruses is not limited to humans, as many animal species, including oysters, which synthesize similar glycan epitopes that act as a gateway for viruses, become vectors for viral infection in humans. Here, we show that different oyster species synthesize a wide range of N-glycans that share histo-blood A-antigens but differ in the expression of other terminal antigens and in their modification by O-methyl groups. In particular, we show that the N-glycans isolated from Crassostrea gigas and Ostrea edulis exhibit exquisite methylation patterns in their terminal N-acetylgalactosamine and fucose residues in terms of position and number, adding another layer of complexity to the post-translational glycosylation modifications of glycoproteins. Furthermore, modeling of the interactions between norovirus capsid proteins and carbohydrate ligands strongly suggests that methylation has the potential to fine-tune the recognition events of oysters by virus particles. MDPI 2023-06-02 /pmc/articles/PMC10301044/ /pubmed/37367667 http://dx.doi.org/10.3390/md21060342 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Auger, Audrey Yu, Shin-Yi Guu, Shih-Yun Quéméner, Agnès Euller-Nicolas, Gabriel Ando, Hiromune Desdouits, Marion Le Guyader, Françoise S. Khoo, Kay-Hooi Le Pendu, Jacques Chirat, Frederic Guerardel, Yann Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title | Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full | Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_fullStr | Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_full_unstemmed | Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_short | Species-Specific N-Glycomes and Methylation Patterns of Oysters Crassostrea gigas and Ostrea edulis and Their Possible Consequences for the Norovirus–HBGA Interaction |
title_sort | species-specific n-glycomes and methylation patterns of oysters crassostrea gigas and ostrea edulis and their possible consequences for the norovirus–hbga interaction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301044/ https://www.ncbi.nlm.nih.gov/pubmed/37367667 http://dx.doi.org/10.3390/md21060342 |
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