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Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks

Galleria mellonella is a promising in vivo model insect used for microbiological, medical, and pharmacological research. It provides a platform for testing the biocompatibility of various compounds and the kinetics of survival after an infection followed by subsequent treatment, and for the evaluati...

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Autores principales: Bugyna, Larysa, Kendra, Samuel, Bujdáková, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301295/
https://www.ncbi.nlm.nih.gov/pubmed/37374956
http://dx.doi.org/10.3390/microorganisms11061455
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author Bugyna, Larysa
Kendra, Samuel
Bujdáková, Helena
author_facet Bugyna, Larysa
Kendra, Samuel
Bujdáková, Helena
author_sort Bugyna, Larysa
collection PubMed
description Galleria mellonella is a promising in vivo model insect used for microbiological, medical, and pharmacological research. It provides a platform for testing the biocompatibility of various compounds and the kinetics of survival after an infection followed by subsequent treatment, and for the evaluation of various parameters during treatment, including the host–pathogen interaction. There are some similarities in the development of pathologies with mammals. However, a limitation is the lack of adaptive immune response. Antimicrobial photodynamic therapy (aPDT) is an alternative approach for combating microbial infections, including biofilm-associated ones. aPDT is effective against Gram-positive and Gram-negative bacteria, viruses, fungi, and parasites, regardless of whether they are resistant to conventional treatment. The main idea of this comprehensive review was to collect information on the use of G. mellonella in aPDT. It provides a collection of references published in the last 10 years from this area of research, complemented by some practical experiences of the authors of this review. Additionally, the review summarizes in brief information on the G. mellonella model, its advantages and methods used in the processing of material from these larvae, as well as basic knowledge of the principles of aPDT.
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spelling pubmed-103012952023-06-29 Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks Bugyna, Larysa Kendra, Samuel Bujdáková, Helena Microorganisms Review Galleria mellonella is a promising in vivo model insect used for microbiological, medical, and pharmacological research. It provides a platform for testing the biocompatibility of various compounds and the kinetics of survival after an infection followed by subsequent treatment, and for the evaluation of various parameters during treatment, including the host–pathogen interaction. There are some similarities in the development of pathologies with mammals. However, a limitation is the lack of adaptive immune response. Antimicrobial photodynamic therapy (aPDT) is an alternative approach for combating microbial infections, including biofilm-associated ones. aPDT is effective against Gram-positive and Gram-negative bacteria, viruses, fungi, and parasites, regardless of whether they are resistant to conventional treatment. The main idea of this comprehensive review was to collect information on the use of G. mellonella in aPDT. It provides a collection of references published in the last 10 years from this area of research, complemented by some practical experiences of the authors of this review. Additionally, the review summarizes in brief information on the G. mellonella model, its advantages and methods used in the processing of material from these larvae, as well as basic knowledge of the principles of aPDT. MDPI 2023-05-31 /pmc/articles/PMC10301295/ /pubmed/37374956 http://dx.doi.org/10.3390/microorganisms11061455 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Bugyna, Larysa
Kendra, Samuel
Bujdáková, Helena
Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks
title Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks
title_full Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks
title_fullStr Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks
title_full_unstemmed Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks
title_short Galleria mellonella—A Model for the Study of aPDT—Prospects and Drawbacks
title_sort galleria mellonella—a model for the study of apdt—prospects and drawbacks
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301295/
https://www.ncbi.nlm.nih.gov/pubmed/37374956
http://dx.doi.org/10.3390/microorganisms11061455
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