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The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301325/ https://www.ncbi.nlm.nih.gov/pubmed/37376309 http://dx.doi.org/10.3390/polym15122663 |
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author | Zdraveva, Emilija Dolenec, Tamara Tominac Trcin, Mirna Govorčin Bajsić, Emi Holjevac Grgurić, Tamara Tomljenović, Antoneta Dekaris, Iva Jelić, Josip Mijovic, Budimir |
author_facet | Zdraveva, Emilija Dolenec, Tamara Tominac Trcin, Mirna Govorčin Bajsić, Emi Holjevac Grgurić, Tamara Tomljenović, Antoneta Dekaris, Iva Jelić, Josip Mijovic, Budimir |
author_sort | Zdraveva, Emilija |
collection | PubMed |
description | Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blockage of abnormal cornea vascularization. In terms of physicochemical properties, the biological component increased the PCL scaffold fiber diameter (by ~24%) and pore area (by ~82%), while ut slightly reduced its total porosity as the anti-VEGF solution filled the voids of the microfibrous structure. The addition of the anti-VEGF increased the scaffold stiffness almost three-fold at both strains of 5 and 10%, as well as its biodegradation rate (~36% after 60 days) with a sustained release profile after Day 4 of phosphate buffered saline incubation. In terms of scaffold application function, the PCL/Anti-VEGF scaffold proved to be more favorable for the adhesion of cultured limbal stem cells (LSCs); this was confirmed by the SEM images, where the cells showed flat and elongated conformations. Further support of the LSC growth and proliferation was confirmed by the identified p63 and CK3 markers after cell staining. These results demonstrate the advantageous effect of the surface-adsorbed anti-VEGF to stop vision loss and help damaged corneal tissue repair. |
format | Online Article Text |
id | pubmed-10301325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103013252023-06-29 The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair Zdraveva, Emilija Dolenec, Tamara Tominac Trcin, Mirna Govorčin Bajsić, Emi Holjevac Grgurić, Tamara Tomljenović, Antoneta Dekaris, Iva Jelić, Josip Mijovic, Budimir Polymers (Basel) Article Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blockage of abnormal cornea vascularization. In terms of physicochemical properties, the biological component increased the PCL scaffold fiber diameter (by ~24%) and pore area (by ~82%), while ut slightly reduced its total porosity as the anti-VEGF solution filled the voids of the microfibrous structure. The addition of the anti-VEGF increased the scaffold stiffness almost three-fold at both strains of 5 and 10%, as well as its biodegradation rate (~36% after 60 days) with a sustained release profile after Day 4 of phosphate buffered saline incubation. In terms of scaffold application function, the PCL/Anti-VEGF scaffold proved to be more favorable for the adhesion of cultured limbal stem cells (LSCs); this was confirmed by the SEM images, where the cells showed flat and elongated conformations. Further support of the LSC growth and proliferation was confirmed by the identified p63 and CK3 markers after cell staining. These results demonstrate the advantageous effect of the surface-adsorbed anti-VEGF to stop vision loss and help damaged corneal tissue repair. MDPI 2023-06-13 /pmc/articles/PMC10301325/ /pubmed/37376309 http://dx.doi.org/10.3390/polym15122663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zdraveva, Emilija Dolenec, Tamara Tominac Trcin, Mirna Govorčin Bajsić, Emi Holjevac Grgurić, Tamara Tomljenović, Antoneta Dekaris, Iva Jelić, Josip Mijovic, Budimir The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair |
title | The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair |
title_full | The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair |
title_fullStr | The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair |
title_full_unstemmed | The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair |
title_short | The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair |
title_sort | reliability of pcl/anti-vegf electrospun scaffolds to support limbal stem cells for corneal repair |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301325/ https://www.ncbi.nlm.nih.gov/pubmed/37376309 http://dx.doi.org/10.3390/polym15122663 |
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