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The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair

Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blo...

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Autores principales: Zdraveva, Emilija, Dolenec, Tamara, Tominac Trcin, Mirna, Govorčin Bajsić, Emi, Holjevac Grgurić, Tamara, Tomljenović, Antoneta, Dekaris, Iva, Jelić, Josip, Mijovic, Budimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301325/
https://www.ncbi.nlm.nih.gov/pubmed/37376309
http://dx.doi.org/10.3390/polym15122663
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author Zdraveva, Emilija
Dolenec, Tamara
Tominac Trcin, Mirna
Govorčin Bajsić, Emi
Holjevac Grgurić, Tamara
Tomljenović, Antoneta
Dekaris, Iva
Jelić, Josip
Mijovic, Budimir
author_facet Zdraveva, Emilija
Dolenec, Tamara
Tominac Trcin, Mirna
Govorčin Bajsić, Emi
Holjevac Grgurić, Tamara
Tomljenović, Antoneta
Dekaris, Iva
Jelić, Josip
Mijovic, Budimir
author_sort Zdraveva, Emilija
collection PubMed
description Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blockage of abnormal cornea vascularization. In terms of physicochemical properties, the biological component increased the PCL scaffold fiber diameter (by ~24%) and pore area (by ~82%), while ut slightly reduced its total porosity as the anti-VEGF solution filled the voids of the microfibrous structure. The addition of the anti-VEGF increased the scaffold stiffness almost three-fold at both strains of 5 and 10%, as well as its biodegradation rate (~36% after 60 days) with a sustained release profile after Day 4 of phosphate buffered saline incubation. In terms of scaffold application function, the PCL/Anti-VEGF scaffold proved to be more favorable for the adhesion of cultured limbal stem cells (LSCs); this was confirmed by the SEM images, where the cells showed flat and elongated conformations. Further support of the LSC growth and proliferation was confirmed by the identified p63 and CK3 markers after cell staining. These results demonstrate the advantageous effect of the surface-adsorbed anti-VEGF to stop vision loss and help damaged corneal tissue repair.
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spelling pubmed-103013252023-06-29 The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair Zdraveva, Emilija Dolenec, Tamara Tominac Trcin, Mirna Govorčin Bajsić, Emi Holjevac Grgurić, Tamara Tomljenović, Antoneta Dekaris, Iva Jelić, Josip Mijovic, Budimir Polymers (Basel) Article Since only few reported studies propose anti-vascular endothelial growth factor (anti-VEGF) delivery through electrospun scaffolds, this study greatly contributes to the potential prevention of patient’s vision loss, as it explores electrospun polycaprolactone (PCL) coated with anti-VEGF for the blockage of abnormal cornea vascularization. In terms of physicochemical properties, the biological component increased the PCL scaffold fiber diameter (by ~24%) and pore area (by ~82%), while ut slightly reduced its total porosity as the anti-VEGF solution filled the voids of the microfibrous structure. The addition of the anti-VEGF increased the scaffold stiffness almost three-fold at both strains of 5 and 10%, as well as its biodegradation rate (~36% after 60 days) with a sustained release profile after Day 4 of phosphate buffered saline incubation. In terms of scaffold application function, the PCL/Anti-VEGF scaffold proved to be more favorable for the adhesion of cultured limbal stem cells (LSCs); this was confirmed by the SEM images, where the cells showed flat and elongated conformations. Further support of the LSC growth and proliferation was confirmed by the identified p63 and CK3 markers after cell staining. These results demonstrate the advantageous effect of the surface-adsorbed anti-VEGF to stop vision loss and help damaged corneal tissue repair. MDPI 2023-06-13 /pmc/articles/PMC10301325/ /pubmed/37376309 http://dx.doi.org/10.3390/polym15122663 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zdraveva, Emilija
Dolenec, Tamara
Tominac Trcin, Mirna
Govorčin Bajsić, Emi
Holjevac Grgurić, Tamara
Tomljenović, Antoneta
Dekaris, Iva
Jelić, Josip
Mijovic, Budimir
The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
title The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
title_full The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
title_fullStr The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
title_full_unstemmed The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
title_short The Reliability of PCL/Anti-VEGF Electrospun Scaffolds to Support Limbal Stem Cells for Corneal Repair
title_sort reliability of pcl/anti-vegf electrospun scaffolds to support limbal stem cells for corneal repair
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301325/
https://www.ncbi.nlm.nih.gov/pubmed/37376309
http://dx.doi.org/10.3390/polym15122663
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