Cargando…
Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications
The therapeutic efficiency of plant extracts has been limited by their poor pharmaceutical availability. Hydrogels have promising potential to be applied as wound dressings due to their high capacity to absorb exudates and their enhanced performance in loading and releasing plant extracts. In this w...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301438/ https://www.ncbi.nlm.nih.gov/pubmed/37376122 http://dx.doi.org/10.3390/pharmaceutics15061674 |
_version_ | 1785064811993759744 |
---|---|
author | Pelin, Irina Mihaela Silion, Mihaela Popescu, Irina Rîmbu, Cristina Mihaela Fundueanu, Gheorghe Constantin, Marieta |
author_facet | Pelin, Irina Mihaela Silion, Mihaela Popescu, Irina Rîmbu, Cristina Mihaela Fundueanu, Gheorghe Constantin, Marieta |
author_sort | Pelin, Irina Mihaela |
collection | PubMed |
description | The therapeutic efficiency of plant extracts has been limited by their poor pharmaceutical availability. Hydrogels have promising potential to be applied as wound dressings due to their high capacity to absorb exudates and their enhanced performance in loading and releasing plant extracts. In this work, pullulan/poly (vinyl alcohol) (P/PVA) hydrogels were first prepared using an eco-friendly method based on both a covalent and physical cross-linking approach. Then, the hydrogels were loaded with the hydroalcoholic extract of Calendula officinalis by a simple post-loading immersion method. Different loading capacities were investigated in terms of the physico-chemical properties, chemical composition, mechanical properties, and water absorption. The hydrogels exhibited high loading efficiency due to the hydrogen bonding interactions between polymer and extract. The water retention capacity as well as the mechanical properties decreased with the increase in the extract amount in hydrogel. However, higher amounts of extract in the hydrogel improved the bioadhesiveness. The release of extract from hydrogels was controlled by the Fickian diffusion mechanism. Extract-loaded hydrogels expressed high antioxidant activity, reaching 70% DPPH radical scavenging after 15 min immersion in buffer solution at pH 5.5. Additionally, loaded hydrogels showed a high antibacterial activity against Gram-positive and Gram-negative bacteria and were non-cytotoxic against HDFa cells. |
format | Online Article Text |
id | pubmed-10301438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103014382023-06-29 Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications Pelin, Irina Mihaela Silion, Mihaela Popescu, Irina Rîmbu, Cristina Mihaela Fundueanu, Gheorghe Constantin, Marieta Pharmaceutics Article The therapeutic efficiency of plant extracts has been limited by their poor pharmaceutical availability. Hydrogels have promising potential to be applied as wound dressings due to their high capacity to absorb exudates and their enhanced performance in loading and releasing plant extracts. In this work, pullulan/poly (vinyl alcohol) (P/PVA) hydrogels were first prepared using an eco-friendly method based on both a covalent and physical cross-linking approach. Then, the hydrogels were loaded with the hydroalcoholic extract of Calendula officinalis by a simple post-loading immersion method. Different loading capacities were investigated in terms of the physico-chemical properties, chemical composition, mechanical properties, and water absorption. The hydrogels exhibited high loading efficiency due to the hydrogen bonding interactions between polymer and extract. The water retention capacity as well as the mechanical properties decreased with the increase in the extract amount in hydrogel. However, higher amounts of extract in the hydrogel improved the bioadhesiveness. The release of extract from hydrogels was controlled by the Fickian diffusion mechanism. Extract-loaded hydrogels expressed high antioxidant activity, reaching 70% DPPH radical scavenging after 15 min immersion in buffer solution at pH 5.5. Additionally, loaded hydrogels showed a high antibacterial activity against Gram-positive and Gram-negative bacteria and were non-cytotoxic against HDFa cells. MDPI 2023-06-07 /pmc/articles/PMC10301438/ /pubmed/37376122 http://dx.doi.org/10.3390/pharmaceutics15061674 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pelin, Irina Mihaela Silion, Mihaela Popescu, Irina Rîmbu, Cristina Mihaela Fundueanu, Gheorghe Constantin, Marieta Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications |
title | Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications |
title_full | Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications |
title_fullStr | Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications |
title_full_unstemmed | Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications |
title_short | Pullulan/Poly(vinyl alcohol) Hydrogels Loaded with Calendula officinalis Extract: Design and In Vitro Evaluation for Wound Healing Applications |
title_sort | pullulan/poly(vinyl alcohol) hydrogels loaded with calendula officinalis extract: design and in vitro evaluation for wound healing applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301438/ https://www.ncbi.nlm.nih.gov/pubmed/37376122 http://dx.doi.org/10.3390/pharmaceutics15061674 |
work_keys_str_mv | AT pelinirinamihaela pullulanpolyvinylalcoholhydrogelsloadedwithcalendulaofficinalisextractdesignandinvitroevaluationforwoundhealingapplications AT silionmihaela pullulanpolyvinylalcoholhydrogelsloadedwithcalendulaofficinalisextractdesignandinvitroevaluationforwoundhealingapplications AT popescuirina pullulanpolyvinylalcoholhydrogelsloadedwithcalendulaofficinalisextractdesignandinvitroevaluationforwoundhealingapplications AT rimbucristinamihaela pullulanpolyvinylalcoholhydrogelsloadedwithcalendulaofficinalisextractdesignandinvitroevaluationforwoundhealingapplications AT fundueanugheorghe pullulanpolyvinylalcoholhydrogelsloadedwithcalendulaofficinalisextractdesignandinvitroevaluationforwoundhealingapplications AT constantinmarieta pullulanpolyvinylalcoholhydrogelsloadedwithcalendulaofficinalisextractdesignandinvitroevaluationforwoundhealingapplications |