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Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms

Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare syst...

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Autores principales: Canellas, Anna Luiza Bauer, de Oliveira, Bruno Francesco Rodrigues, Nunes, Suzanne de Oliveira, Malafaia, Camila Adão, Amaral, Ana Claudia F., Simas, Daniel Luiz Reis, Leal, Ivana Correa Ramos, Laport, Marinella Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301669/
https://www.ncbi.nlm.nih.gov/pubmed/37375398
http://dx.doi.org/10.3390/molecules28124843
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author Canellas, Anna Luiza Bauer
de Oliveira, Bruno Francesco Rodrigues
Nunes, Suzanne de Oliveira
Malafaia, Camila Adão
Amaral, Ana Claudia F.
Simas, Daniel Luiz Reis
Leal, Ivana Correa Ramos
Laport, Marinella Silva
author_facet Canellas, Anna Luiza Bauer
de Oliveira, Bruno Francesco Rodrigues
Nunes, Suzanne de Oliveira
Malafaia, Camila Adão
Amaral, Ana Claudia F.
Simas, Daniel Luiz Reis
Leal, Ivana Correa Ramos
Laport, Marinella Silva
author_sort Canellas, Anna Luiza Bauer
collection PubMed
description Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated Enterobacter sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of Enterobacter sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived Enterobacter as a source of antibiofilm compounds.
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spelling pubmed-103016692023-06-29 Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms Canellas, Anna Luiza Bauer de Oliveira, Bruno Francesco Rodrigues Nunes, Suzanne de Oliveira Malafaia, Camila Adão Amaral, Ana Claudia F. Simas, Daniel Luiz Reis Leal, Ivana Correa Ramos Laport, Marinella Silva Molecules Article Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated Enterobacter sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of Enterobacter sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived Enterobacter as a source of antibiofilm compounds. MDPI 2023-06-19 /pmc/articles/PMC10301669/ /pubmed/37375398 http://dx.doi.org/10.3390/molecules28124843 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Canellas, Anna Luiza Bauer
de Oliveira, Bruno Francesco Rodrigues
Nunes, Suzanne de Oliveira
Malafaia, Camila Adão
Amaral, Ana Claudia F.
Simas, Daniel Luiz Reis
Leal, Ivana Correa Ramos
Laport, Marinella Silva
Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
title Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
title_full Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
title_fullStr Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
title_full_unstemmed Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
title_short Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
title_sort delving into the mechanisms of sponge-associated enterobacter against staphylococcal biofilms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301669/
https://www.ncbi.nlm.nih.gov/pubmed/37375398
http://dx.doi.org/10.3390/molecules28124843
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