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Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms
Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare syst...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301669/ https://www.ncbi.nlm.nih.gov/pubmed/37375398 http://dx.doi.org/10.3390/molecules28124843 |
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author | Canellas, Anna Luiza Bauer de Oliveira, Bruno Francesco Rodrigues Nunes, Suzanne de Oliveira Malafaia, Camila Adão Amaral, Ana Claudia F. Simas, Daniel Luiz Reis Leal, Ivana Correa Ramos Laport, Marinella Silva |
author_facet | Canellas, Anna Luiza Bauer de Oliveira, Bruno Francesco Rodrigues Nunes, Suzanne de Oliveira Malafaia, Camila Adão Amaral, Ana Claudia F. Simas, Daniel Luiz Reis Leal, Ivana Correa Ramos Laport, Marinella Silva |
author_sort | Canellas, Anna Luiza Bauer |
collection | PubMed |
description | Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated Enterobacter sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of Enterobacter sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived Enterobacter as a source of antibiofilm compounds. |
format | Online Article Text |
id | pubmed-10301669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103016692023-06-29 Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms Canellas, Anna Luiza Bauer de Oliveira, Bruno Francesco Rodrigues Nunes, Suzanne de Oliveira Malafaia, Camila Adão Amaral, Ana Claudia F. Simas, Daniel Luiz Reis Leal, Ivana Correa Ramos Laport, Marinella Silva Molecules Article Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated Enterobacter sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of Enterobacter sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived Enterobacter as a source of antibiofilm compounds. MDPI 2023-06-19 /pmc/articles/PMC10301669/ /pubmed/37375398 http://dx.doi.org/10.3390/molecules28124843 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Canellas, Anna Luiza Bauer de Oliveira, Bruno Francesco Rodrigues Nunes, Suzanne de Oliveira Malafaia, Camila Adão Amaral, Ana Claudia F. Simas, Daniel Luiz Reis Leal, Ivana Correa Ramos Laport, Marinella Silva Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms |
title | Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms |
title_full | Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms |
title_fullStr | Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms |
title_full_unstemmed | Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms |
title_short | Delving into the Mechanisms of Sponge-Associated Enterobacter against Staphylococcal Biofilms |
title_sort | delving into the mechanisms of sponge-associated enterobacter against staphylococcal biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301669/ https://www.ncbi.nlm.nih.gov/pubmed/37375398 http://dx.doi.org/10.3390/molecules28124843 |
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