Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue

Cyanobacteria produce a variety of secondary metabolites, including toxins that may contribute to the development of disease. Previous work was able to detect the presence of a cyanobacterial marker in human nasal and broncoalveolar lavage samples; however, it was not able to determine the quantific...

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Autores principales: Barney, Rachael E., Huang, Guohong, Gallagher, Torrey L., Tischbein, Maeve, DeWitt, John, Martindale, Rachel, LaRochelle, Ethan M. P., Tsongalis, Gregory J., Stommel, Elijah W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302173/
https://www.ncbi.nlm.nih.gov/pubmed/37368631
http://dx.doi.org/10.3390/toxics11060531
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author Barney, Rachael E.
Huang, Guohong
Gallagher, Torrey L.
Tischbein, Maeve
DeWitt, John
Martindale, Rachel
LaRochelle, Ethan M. P.
Tsongalis, Gregory J.
Stommel, Elijah W.
author_facet Barney, Rachael E.
Huang, Guohong
Gallagher, Torrey L.
Tischbein, Maeve
DeWitt, John
Martindale, Rachel
LaRochelle, Ethan M. P.
Tsongalis, Gregory J.
Stommel, Elijah W.
author_sort Barney, Rachael E.
collection PubMed
description Cyanobacteria produce a variety of secondary metabolites, including toxins that may contribute to the development of disease. Previous work was able to detect the presence of a cyanobacterial marker in human nasal and broncoalveolar lavage samples; however, it was not able to determine the quantification of the marker. To further research the relationship between cyanobacteria and human health, we validated a droplet digital polymerase chain reaction (ddPCR) assay to simultaneously detect the cyanobacterial 16S marker and a human housekeeping gene in human lung tissue samples. The ability to detect cyanobacteria in human samples will allow further research into the role cyanobacteria plays in human health and disease.
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spelling pubmed-103021732023-06-29 Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue Barney, Rachael E. Huang, Guohong Gallagher, Torrey L. Tischbein, Maeve DeWitt, John Martindale, Rachel LaRochelle, Ethan M. P. Tsongalis, Gregory J. Stommel, Elijah W. Toxics Technical Note Cyanobacteria produce a variety of secondary metabolites, including toxins that may contribute to the development of disease. Previous work was able to detect the presence of a cyanobacterial marker in human nasal and broncoalveolar lavage samples; however, it was not able to determine the quantification of the marker. To further research the relationship between cyanobacteria and human health, we validated a droplet digital polymerase chain reaction (ddPCR) assay to simultaneously detect the cyanobacterial 16S marker and a human housekeeping gene in human lung tissue samples. The ability to detect cyanobacteria in human samples will allow further research into the role cyanobacteria plays in human health and disease. MDPI 2023-06-14 /pmc/articles/PMC10302173/ /pubmed/37368631 http://dx.doi.org/10.3390/toxics11060531 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Barney, Rachael E.
Huang, Guohong
Gallagher, Torrey L.
Tischbein, Maeve
DeWitt, John
Martindale, Rachel
LaRochelle, Ethan M. P.
Tsongalis, Gregory J.
Stommel, Elijah W.
Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
title Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
title_full Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
title_fullStr Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
title_full_unstemmed Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
title_short Validation of a Droplet Digital PCR (ddPCR) Assay to Detect Cyanobacterial 16S rDNA in Human Lung Tissue
title_sort validation of a droplet digital pcr (ddpcr) assay to detect cyanobacterial 16s rdna in human lung tissue
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302173/
https://www.ncbi.nlm.nih.gov/pubmed/37368631
http://dx.doi.org/10.3390/toxics11060531
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