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Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control

Mosquitoes are vectors of many pathogens, including viruses, protozoans, and helminths, spreading these pathogens to humans as well as to wild and domestic animals. As the identification of species and the biological characterization of mosquito vectors are cornerstones for understanding patterns of...

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Autores principales: Meireles, Anne Caroline Alves, Rios, Flávia Geovana Fontineles, Feitoza, Luiz Henrique Maciel, da Silva, Lucas Rosendo, Julião, Genimar Rebouças
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302199/
https://www.ncbi.nlm.nih.gov/pubmed/37375506
http://dx.doi.org/10.3390/pathogens12060816
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author Meireles, Anne Caroline Alves
Rios, Flávia Geovana Fontineles
Feitoza, Luiz Henrique Maciel
da Silva, Lucas Rosendo
Julião, Genimar Rebouças
author_facet Meireles, Anne Caroline Alves
Rios, Flávia Geovana Fontineles
Feitoza, Luiz Henrique Maciel
da Silva, Lucas Rosendo
Julião, Genimar Rebouças
author_sort Meireles, Anne Caroline Alves
collection PubMed
description Mosquitoes are vectors of many pathogens, including viruses, protozoans, and helminths, spreading these pathogens to humans as well as to wild and domestic animals. As the identification of species and the biological characterization of mosquito vectors are cornerstones for understanding patterns of disease transmission, and the design of control strategies, we conducted a literature review on the current use of noninvasive and nondestructive techniques for pathogen detection in mosquitoes, highlighting the importance of their taxonomic status and systematics, and some gaps in the knowledge of their vectorial capacity. Here, we summarized the alternative techniques for pathogen detection in mosquitoes based on both laboratory and field studies. Parasite infection and dissemination by mosquitoes can also be obtained via analyses of saliva- and excreta-based techniques or of the whole mosquito body, using a near-infrared spectrometry (NIRS) approach. Further research should be encouraged to seek strategies for detecting target pathogens while preserving mosquito morphology, especially in biodiversity hotspot regions, thus enabling the discovery of cryptic or new species, and the determination of more accurate taxonomic, parasitological, and epidemiological patterns.
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spelling pubmed-103021992023-06-29 Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control Meireles, Anne Caroline Alves Rios, Flávia Geovana Fontineles Feitoza, Luiz Henrique Maciel da Silva, Lucas Rosendo Julião, Genimar Rebouças Pathogens Review Mosquitoes are vectors of many pathogens, including viruses, protozoans, and helminths, spreading these pathogens to humans as well as to wild and domestic animals. As the identification of species and the biological characterization of mosquito vectors are cornerstones for understanding patterns of disease transmission, and the design of control strategies, we conducted a literature review on the current use of noninvasive and nondestructive techniques for pathogen detection in mosquitoes, highlighting the importance of their taxonomic status and systematics, and some gaps in the knowledge of their vectorial capacity. Here, we summarized the alternative techniques for pathogen detection in mosquitoes based on both laboratory and field studies. Parasite infection and dissemination by mosquitoes can also be obtained via analyses of saliva- and excreta-based techniques or of the whole mosquito body, using a near-infrared spectrometry (NIRS) approach. Further research should be encouraged to seek strategies for detecting target pathogens while preserving mosquito morphology, especially in biodiversity hotspot regions, thus enabling the discovery of cryptic or new species, and the determination of more accurate taxonomic, parasitological, and epidemiological patterns. MDPI 2023-06-08 /pmc/articles/PMC10302199/ /pubmed/37375506 http://dx.doi.org/10.3390/pathogens12060816 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Meireles, Anne Caroline Alves
Rios, Flávia Geovana Fontineles
Feitoza, Luiz Henrique Maciel
da Silva, Lucas Rosendo
Julião, Genimar Rebouças
Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control
title Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control
title_full Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control
title_fullStr Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control
title_full_unstemmed Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control
title_short Nondestructive Methods of Pathogen Detection: Importance of Mosquito Integrity in Studies of Disease Transmission and Control
title_sort nondestructive methods of pathogen detection: importance of mosquito integrity in studies of disease transmission and control
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302199/
https://www.ncbi.nlm.nih.gov/pubmed/37375506
http://dx.doi.org/10.3390/pathogens12060816
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