Cargando…

Non-Targeted Metabolomic Study of Fetal Growth Restriction

We aimed to explore the differential metabolites in amniotic fluid and its cells from fetuses with fetal growth restriction (FGR). A total of 28 specimens of amniotic fluid were collected, including 18 with FGR and 10 controls. Differential metabolites in all samples were detected by chromatography–...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Fang, Li, Zhi, Xu, Yanwen, Huang, Shuang, Li, Yanqiu, Jiang, Weiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302415/
https://www.ncbi.nlm.nih.gov/pubmed/37367917
http://dx.doi.org/10.3390/metabo13060761
_version_ 1785065039510634496
author Chen, Fang
Li, Zhi
Xu, Yanwen
Huang, Shuang
Li, Yanqiu
Jiang, Weiying
author_facet Chen, Fang
Li, Zhi
Xu, Yanwen
Huang, Shuang
Li, Yanqiu
Jiang, Weiying
author_sort Chen, Fang
collection PubMed
description We aimed to explore the differential metabolites in amniotic fluid and its cells from fetuses with fetal growth restriction (FGR). A total of 28 specimens of amniotic fluid were collected, including 18 with FGR and 10 controls. Differential metabolites in all samples were detected by chromatography–mass spectrometry. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to analyze the differences in metabolic spectra between the FGR and control groups through multidimensional and single-dimensional statistical analysis. The KEGG database was used for metabolic pathway enrichment analysis. Both PCA and OPLS-DA models showed a clear separation trend between FGR and control groups. We identified 27 differentially expressed metabolites in the amniotic fluid supernatant of the two groups (p < 0.05), of which 14 metabolites were up-regulated in the FGR group, and 13 metabolites, such as glutamate, phenylalanine, valine and leucine, were down-regulated. We also identified 20 differentially expressed metabolites in the amniotic fluid cell (p < 0.05), of which 9 metabolites, including malic acid, glycolic acid and D-glycerate, were up-regulated significantly and 11 metabolites, including glyceraldehyde, were down-regulated. Pathway analysis showed that most of the identified differential metabolites were involved in tricarboxylic acid cycle (TCA cycle), ABC transport, amino acid metabolism pathways and so on. The results indicated that many metabolic changes associated with FGR, which are mainly manifested by abnormal metabolism of amino acid in amniotic fluid and abnormal glucose metabolism including TCA cycle in amniotic fluid cells, respectively. Our findings provide more data for exploring the mechanism of FGR and the potential therapy targets.
format Online
Article
Text
id pubmed-10302415
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103024152023-06-29 Non-Targeted Metabolomic Study of Fetal Growth Restriction Chen, Fang Li, Zhi Xu, Yanwen Huang, Shuang Li, Yanqiu Jiang, Weiying Metabolites Article We aimed to explore the differential metabolites in amniotic fluid and its cells from fetuses with fetal growth restriction (FGR). A total of 28 specimens of amniotic fluid were collected, including 18 with FGR and 10 controls. Differential metabolites in all samples were detected by chromatography–mass spectrometry. Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used to analyze the differences in metabolic spectra between the FGR and control groups through multidimensional and single-dimensional statistical analysis. The KEGG database was used for metabolic pathway enrichment analysis. Both PCA and OPLS-DA models showed a clear separation trend between FGR and control groups. We identified 27 differentially expressed metabolites in the amniotic fluid supernatant of the two groups (p < 0.05), of which 14 metabolites were up-regulated in the FGR group, and 13 metabolites, such as glutamate, phenylalanine, valine and leucine, were down-regulated. We also identified 20 differentially expressed metabolites in the amniotic fluid cell (p < 0.05), of which 9 metabolites, including malic acid, glycolic acid and D-glycerate, were up-regulated significantly and 11 metabolites, including glyceraldehyde, were down-regulated. Pathway analysis showed that most of the identified differential metabolites were involved in tricarboxylic acid cycle (TCA cycle), ABC transport, amino acid metabolism pathways and so on. The results indicated that many metabolic changes associated with FGR, which are mainly manifested by abnormal metabolism of amino acid in amniotic fluid and abnormal glucose metabolism including TCA cycle in amniotic fluid cells, respectively. Our findings provide more data for exploring the mechanism of FGR and the potential therapy targets. MDPI 2023-06-17 /pmc/articles/PMC10302415/ /pubmed/37367917 http://dx.doi.org/10.3390/metabo13060761 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Fang
Li, Zhi
Xu, Yanwen
Huang, Shuang
Li, Yanqiu
Jiang, Weiying
Non-Targeted Metabolomic Study of Fetal Growth Restriction
title Non-Targeted Metabolomic Study of Fetal Growth Restriction
title_full Non-Targeted Metabolomic Study of Fetal Growth Restriction
title_fullStr Non-Targeted Metabolomic Study of Fetal Growth Restriction
title_full_unstemmed Non-Targeted Metabolomic Study of Fetal Growth Restriction
title_short Non-Targeted Metabolomic Study of Fetal Growth Restriction
title_sort non-targeted metabolomic study of fetal growth restriction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10302415/
https://www.ncbi.nlm.nih.gov/pubmed/37367917
http://dx.doi.org/10.3390/metabo13060761
work_keys_str_mv AT chenfang nontargetedmetabolomicstudyoffetalgrowthrestriction
AT lizhi nontargetedmetabolomicstudyoffetalgrowthrestriction
AT xuyanwen nontargetedmetabolomicstudyoffetalgrowthrestriction
AT huangshuang nontargetedmetabolomicstudyoffetalgrowthrestriction
AT liyanqiu nontargetedmetabolomicstudyoffetalgrowthrestriction
AT jiangweiying nontargetedmetabolomicstudyoffetalgrowthrestriction