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Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor

Due to their capacity to produce antimicrobial peptides that can prevent the growth of diseases, many Bacillus spp. are beneficial to plants. In this study, we looked into the antagonistic activity of the B. pumilus 3-19 strain and its derivatives following targeted genome editing. Two peptide genes...

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Autores principales: Danilova, Iuliia V., Vasileva, Iuliia A., Gilmutdinova, Ajgul I., Dyadkina, Ilona V., Khusnullina, Liya K., Khasanov, Damir I., Rudakova, Natalia L., Sharipova, Margarita R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303315/
https://www.ncbi.nlm.nih.gov/pubmed/37375011
http://dx.doi.org/10.3390/microorganisms11061508
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author Danilova, Iuliia V.
Vasileva, Iuliia A.
Gilmutdinova, Ajgul I.
Dyadkina, Ilona V.
Khusnullina, Liya K.
Khasanov, Damir I.
Rudakova, Natalia L.
Sharipova, Margarita R.
author_facet Danilova, Iuliia V.
Vasileva, Iuliia A.
Gilmutdinova, Ajgul I.
Dyadkina, Ilona V.
Khusnullina, Liya K.
Khasanov, Damir I.
Rudakova, Natalia L.
Sharipova, Margarita R.
author_sort Danilova, Iuliia V.
collection PubMed
description Due to their capacity to produce antimicrobial peptides that can prevent the growth of diseases, many Bacillus spp. are beneficial to plants. In this study, we looked into the antagonistic activity of the B. pumilus 3-19 strain and its derivatives following targeted genome editing. Two peptide genes with antibacterial action, bacilysin (bac) and bacteriocin (bact), and the sigF gene, which encodes the sigma factor of sporulation, were specifically inactivated using the CRISPR-Cas9 system in the genome of B. pumilus 3-19. Antibacterial activity against B. cereus and Pantoea brenneri decreased as a result of the inactivation of target genes in the B. pumilus 3-19 genome, with a noticeable effect against bacilysin. The growth dynamics of the culture changed when the bac, bact, and sigF genes were inactivated, and the altered strains had less proteolytic activity. An asporogenic mutant of B. pumilus 3-19 was obtained by inactivating the sigF gene. It has been proven that bacilysin plays a unique part in the development of B. pumilus 3-19’s antagonistic action against soil microorganisms.
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spelling pubmed-103033152023-06-29 Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor Danilova, Iuliia V. Vasileva, Iuliia A. Gilmutdinova, Ajgul I. Dyadkina, Ilona V. Khusnullina, Liya K. Khasanov, Damir I. Rudakova, Natalia L. Sharipova, Margarita R. Microorganisms Article Due to their capacity to produce antimicrobial peptides that can prevent the growth of diseases, many Bacillus spp. are beneficial to plants. In this study, we looked into the antagonistic activity of the B. pumilus 3-19 strain and its derivatives following targeted genome editing. Two peptide genes with antibacterial action, bacilysin (bac) and bacteriocin (bact), and the sigF gene, which encodes the sigma factor of sporulation, were specifically inactivated using the CRISPR-Cas9 system in the genome of B. pumilus 3-19. Antibacterial activity against B. cereus and Pantoea brenneri decreased as a result of the inactivation of target genes in the B. pumilus 3-19 genome, with a noticeable effect against bacilysin. The growth dynamics of the culture changed when the bac, bact, and sigF genes were inactivated, and the altered strains had less proteolytic activity. An asporogenic mutant of B. pumilus 3-19 was obtained by inactivating the sigF gene. It has been proven that bacilysin plays a unique part in the development of B. pumilus 3-19’s antagonistic action against soil microorganisms. MDPI 2023-06-06 /pmc/articles/PMC10303315/ /pubmed/37375011 http://dx.doi.org/10.3390/microorganisms11061508 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Danilova, Iuliia V.
Vasileva, Iuliia A.
Gilmutdinova, Ajgul I.
Dyadkina, Ilona V.
Khusnullina, Liya K.
Khasanov, Damir I.
Rudakova, Natalia L.
Sharipova, Margarita R.
Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor
title Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor
title_full Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor
title_fullStr Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor
title_full_unstemmed Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor
title_short Characterization of Bacillus pumilus Strains with Targeted Gene Editing for Antimicrobial Peptides and Sporulation Factor
title_sort characterization of bacillus pumilus strains with targeted gene editing for antimicrobial peptides and sporulation factor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303315/
https://www.ncbi.nlm.nih.gov/pubmed/37375011
http://dx.doi.org/10.3390/microorganisms11061508
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