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Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production
The in vitro cultures of Rindera graeca, a rare endemic plant, were developed as a sustainable source of phenolic acids. Various shoot and root cultures were established and scaled up in a sprinkle bioreactor. A multiplication rate of 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis r...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303423/ https://www.ncbi.nlm.nih.gov/pubmed/37375435 http://dx.doi.org/10.3390/molecules28124880 |
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author | Sykłowska-Baranek, Katarzyna Gaweł, Małgorzata Kuźma, Łukasz Wileńska, Beata Kawka, Mateusz Jeziorek, Małgorzata Graikou, Konstantia Chinou, Ioanna Szyszko, Ewa Stępień, Piotr Zakrzewski, Patryk Pietrosiuk, Agnieszka |
author_facet | Sykłowska-Baranek, Katarzyna Gaweł, Małgorzata Kuźma, Łukasz Wileńska, Beata Kawka, Mateusz Jeziorek, Małgorzata Graikou, Konstantia Chinou, Ioanna Szyszko, Ewa Stępień, Piotr Zakrzewski, Patryk Pietrosiuk, Agnieszka |
author_sort | Sykłowska-Baranek, Katarzyna |
collection | PubMed |
description | The in vitro cultures of Rindera graeca, a rare endemic plant, were developed as a sustainable source of phenolic acids. Various shoot and root cultures were established and scaled up in a sprinkle bioreactor. A multiplication rate of 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis revealed the presence of rosmarinic acid (RA) and lithospermic acid B (LAB) as the main secondary metabolites in both the shoot and root cultures. The maximum RA (30.0 ± 3.2 mg/g DW) and LAB (49.3 ± 15.5 mg/g DW) yields were determined in root-regenerated shoots. The strongest free radical scavenging activity (87.4 ± 1.1%), according to 2,2-diphenyl-1-picrylhydrazyl-hydrate assay, was noted for roots cultivated in a DCR medium. The highest reducing power (2.3 µM ± 0.4 TE/g DW), determined by the ferric-reducing antioxidant power assay, was noted for shoots cultivated on an SH medium containing 0.5 mg/L 6-benzylaminopurine. A genetic analysis performed using random amplified polymorphic DNA and start codon targeted markers revealed genetic variation of 62.8% to 96.5% among the investigated shoots and roots. This variability reflects the capacity of cultivated shoots and roots to produce phenolic compounds. |
format | Online Article Text |
id | pubmed-10303423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103034232023-06-29 Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production Sykłowska-Baranek, Katarzyna Gaweł, Małgorzata Kuźma, Łukasz Wileńska, Beata Kawka, Mateusz Jeziorek, Małgorzata Graikou, Konstantia Chinou, Ioanna Szyszko, Ewa Stępień, Piotr Zakrzewski, Patryk Pietrosiuk, Agnieszka Molecules Article The in vitro cultures of Rindera graeca, a rare endemic plant, were developed as a sustainable source of phenolic acids. Various shoot and root cultures were established and scaled up in a sprinkle bioreactor. A multiplication rate of 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis revealed the presence of rosmarinic acid (RA) and lithospermic acid B (LAB) as the main secondary metabolites in both the shoot and root cultures. The maximum RA (30.0 ± 3.2 mg/g DW) and LAB (49.3 ± 15.5 mg/g DW) yields were determined in root-regenerated shoots. The strongest free radical scavenging activity (87.4 ± 1.1%), according to 2,2-diphenyl-1-picrylhydrazyl-hydrate assay, was noted for roots cultivated in a DCR medium. The highest reducing power (2.3 µM ± 0.4 TE/g DW), determined by the ferric-reducing antioxidant power assay, was noted for shoots cultivated on an SH medium containing 0.5 mg/L 6-benzylaminopurine. A genetic analysis performed using random amplified polymorphic DNA and start codon targeted markers revealed genetic variation of 62.8% to 96.5% among the investigated shoots and roots. This variability reflects the capacity of cultivated shoots and roots to produce phenolic compounds. MDPI 2023-06-20 /pmc/articles/PMC10303423/ /pubmed/37375435 http://dx.doi.org/10.3390/molecules28124880 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sykłowska-Baranek, Katarzyna Gaweł, Małgorzata Kuźma, Łukasz Wileńska, Beata Kawka, Mateusz Jeziorek, Małgorzata Graikou, Konstantia Chinou, Ioanna Szyszko, Ewa Stępień, Piotr Zakrzewski, Patryk Pietrosiuk, Agnieszka Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production |
title | Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production |
title_full | Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production |
title_fullStr | Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production |
title_full_unstemmed | Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production |
title_short | Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production |
title_sort | rindera graeca (a. dc.) boiss. & heldr. (boraginaceae) in vitro cultures targeting lithospermic acid b and rosmarinic acid production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303423/ https://www.ncbi.nlm.nih.gov/pubmed/37375435 http://dx.doi.org/10.3390/molecules28124880 |
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