Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis

Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in th...

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Detalles Bibliográficos
Autores principales: Bocova, Ledio, Hubens, Wouter, Engel, Cordula, Koschmieder, Steffen, Jost, Edgar, Wagner, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303729/
https://www.ncbi.nlm.nih.gov/pubmed/37370186
http://dx.doi.org/10.1186/s13148-023-01521-w
Descripción
Sumario:Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in the genes MYO1D, STK17A, and SP140 correlated with CD34(+) cell numbers in mobilized peripheral blood and with blast counts in leukemia. In the future, such epigenetic biomarkers can support the evaluation of stem cell mobilization, HSPC harvesting, or blast count in leukemia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-023-01521-w.

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