Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis

Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in th...

Descripción completa

Detalles Bibliográficos
Autores principales: Bocova, Ledio, Hubens, Wouter, Engel, Cordula, Koschmieder, Steffen, Jost, Edgar, Wagner, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303729/
https://www.ncbi.nlm.nih.gov/pubmed/37370186
http://dx.doi.org/10.1186/s13148-023-01521-w
_version_ 1785065344471138304
author Bocova, Ledio
Hubens, Wouter
Engel, Cordula
Koschmieder, Steffen
Jost, Edgar
Wagner, Wolfgang
author_facet Bocova, Ledio
Hubens, Wouter
Engel, Cordula
Koschmieder, Steffen
Jost, Edgar
Wagner, Wolfgang
author_sort Bocova, Ledio
collection PubMed
description Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in the genes MYO1D, STK17A, and SP140 correlated with CD34(+) cell numbers in mobilized peripheral blood and with blast counts in leukemia. In the future, such epigenetic biomarkers can support the evaluation of stem cell mobilization, HSPC harvesting, or blast count in leukemia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-023-01521-w.
format Online
Article
Text
id pubmed-10303729
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-103037292023-06-29 Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis Bocova, Ledio Hubens, Wouter Engel, Cordula Koschmieder, Steffen Jost, Edgar Wagner, Wolfgang Clin Epigenetics Brief Report Hematopoietic stem and progenitor cells (HSPCs) are quantified in daily clinical practice by flow cytometry. In this study, we provide proof of concept that HSPCs can also be estimated by targeted DNA methylation (DNAm) analysis. The DNAm levels at three individual CG dinucleotides (CpG sites) in the genes MYO1D, STK17A, and SP140 correlated with CD34(+) cell numbers in mobilized peripheral blood and with blast counts in leukemia. In the future, such epigenetic biomarkers can support the evaluation of stem cell mobilization, HSPC harvesting, or blast count in leukemia. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-023-01521-w. BioMed Central 2023-06-27 /pmc/articles/PMC10303729/ /pubmed/37370186 http://dx.doi.org/10.1186/s13148-023-01521-w Text en © The Author(s) 2023, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Brief Report
Bocova, Ledio
Hubens, Wouter
Engel, Cordula
Koschmieder, Steffen
Jost, Edgar
Wagner, Wolfgang
Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
title Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
title_full Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
title_fullStr Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
title_full_unstemmed Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
title_short Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
title_sort quantification of hematopoietic stem and progenitor cells by targeted dna methylation analysis
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303729/
https://www.ncbi.nlm.nih.gov/pubmed/37370186
http://dx.doi.org/10.1186/s13148-023-01521-w
work_keys_str_mv AT bocovaledio quantificationofhematopoieticstemandprogenitorcellsbytargeteddnamethylationanalysis
AT hubenswouter quantificationofhematopoieticstemandprogenitorcellsbytargeteddnamethylationanalysis
AT engelcordula quantificationofhematopoieticstemandprogenitorcellsbytargeteddnamethylationanalysis
AT koschmiedersteffen quantificationofhematopoieticstemandprogenitorcellsbytargeteddnamethylationanalysis
AT jostedgar quantificationofhematopoieticstemandprogenitorcellsbytargeteddnamethylationanalysis
AT wagnerwolfgang quantificationofhematopoieticstemandprogenitorcellsbytargeteddnamethylationanalysis

Ejemplares similares