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In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)

BACKGROUND: Dacryodes edulis is a plant that belongs to the Burseraceae family. It is widely used traditionally alone or in association with other plants in Cameroonian folk medicine to cure wounds, fever, headaches, and malaria. The aim of this work was to investigate the leaves and stem bark of D....

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Autores principales: Dongmo, Kevine Johane Jumeta, Tali, Mariscal Brice Tchatat, Fongang, Yannick Stéphane Fotsing, Taguimjeu, Pierre Leonel K. Tafokeu, Kagho, Donald Ulrich Kenou, Bitchagno, Gabin Thierry, Lenta, Bruno Ndjakou, Boyom, Fabrice Fekam, Sewald, Norbert, Ngouela, Silvère Augustin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303846/
https://www.ncbi.nlm.nih.gov/pubmed/37370061
http://dx.doi.org/10.1186/s12906-023-03957-2
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author Dongmo, Kevine Johane Jumeta
Tali, Mariscal Brice Tchatat
Fongang, Yannick Stéphane Fotsing
Taguimjeu, Pierre Leonel K. Tafokeu
Kagho, Donald Ulrich Kenou
Bitchagno, Gabin Thierry
Lenta, Bruno Ndjakou
Boyom, Fabrice Fekam
Sewald, Norbert
Ngouela, Silvère Augustin
author_facet Dongmo, Kevine Johane Jumeta
Tali, Mariscal Brice Tchatat
Fongang, Yannick Stéphane Fotsing
Taguimjeu, Pierre Leonel K. Tafokeu
Kagho, Donald Ulrich Kenou
Bitchagno, Gabin Thierry
Lenta, Bruno Ndjakou
Boyom, Fabrice Fekam
Sewald, Norbert
Ngouela, Silvère Augustin
author_sort Dongmo, Kevine Johane Jumeta
collection PubMed
description BACKGROUND: Dacryodes edulis is a plant that belongs to the Burseraceae family. It is widely used traditionally alone or in association with other plants in Cameroonian folk medicine to cure wounds, fever, headaches, and malaria. The aim of this work was to investigate the leaves and stem bark of D. edulis with an emphasis on the antiplasmodial and cytotoxic effects of extracts, fractions, and isolated compounds. METHODS: Extracts, fractions, and some isolated compounds were subjected to antiplasmodial activity screening in vitro against chloroquine-sensitive 3D7 and multidrug resistant Dd2 strains of Plasmodium falciparum using a SyBr Green fluorescence-based assay. The cytotoxicity of active extracts, fractions, and compounds was tested against mammalian Raw cell lines using an in vitro resazurin-based viability assay. The structures of the compounds were determined based on their NMR and MS data. The in vivo toxicity using female BALB/c mice was performed on the most active extract according to the protocol of OECD (2002), guideline 423. RESULTS: The hydroethanolic extract from the leaves of D. edulis displayed good antiplasmodial activity with IC(50) values of 3.10 and 3.56 μg/mL respectively on sensitive (3D7) and multiresistant (Dd2) strains of P. falciparum. Of the sixteen compounds isolated, 3,3′,4-tri-O-methylellagic acid (4) exhibited the highest antiplasmodial activity against PfDd2 strains with an IC(50) value of 0.63 μg/mL. All extracts, fractions, and isolated compounds demonstrated no cytotoxicity against Raw cell lines with CC(50) > 250 μg/mL. In addition, the most active extract on both strains of P. falciparum was nontoxic in vivo, with a LD(50) greater than 2000 and 5000 mg/kg. A phytochemical investigation of the stem bark and leaves of D. edulis afforded sixteen compounds, including two xanthones (1–2), three ellagic acid derivatives (3–5), one phenolic compound (6), one depside (7), one triglyceride (8), one auranthiamide acetate (9), one gallic acid derivative (10), four triterpenoids (11–14), and two steroids (15–16). Compounds 1, 2, 5, 7, 8, and 9 were herein reported for the first time from the Burseraceae family. CONCLUSION: This work highlights the good in vitro antiplasmodial potency of the hydroethanolic extract of the leaves of this plant and that of two isolated constituents (3,3′,4-tri-O-methylellagic acid and ethylgallate) from the plant. These biological results support the use of D. edulis in traditional medicine against malaria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-023-03957-2.
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spelling pubmed-103038462023-06-29 In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae) Dongmo, Kevine Johane Jumeta Tali, Mariscal Brice Tchatat Fongang, Yannick Stéphane Fotsing Taguimjeu, Pierre Leonel K. Tafokeu Kagho, Donald Ulrich Kenou Bitchagno, Gabin Thierry Lenta, Bruno Ndjakou Boyom, Fabrice Fekam Sewald, Norbert Ngouela, Silvère Augustin BMC Complement Med Ther Research BACKGROUND: Dacryodes edulis is a plant that belongs to the Burseraceae family. It is widely used traditionally alone or in association with other plants in Cameroonian folk medicine to cure wounds, fever, headaches, and malaria. The aim of this work was to investigate the leaves and stem bark of D. edulis with an emphasis on the antiplasmodial and cytotoxic effects of extracts, fractions, and isolated compounds. METHODS: Extracts, fractions, and some isolated compounds were subjected to antiplasmodial activity screening in vitro against chloroquine-sensitive 3D7 and multidrug resistant Dd2 strains of Plasmodium falciparum using a SyBr Green fluorescence-based assay. The cytotoxicity of active extracts, fractions, and compounds was tested against mammalian Raw cell lines using an in vitro resazurin-based viability assay. The structures of the compounds were determined based on their NMR and MS data. The in vivo toxicity using female BALB/c mice was performed on the most active extract according to the protocol of OECD (2002), guideline 423. RESULTS: The hydroethanolic extract from the leaves of D. edulis displayed good antiplasmodial activity with IC(50) values of 3.10 and 3.56 μg/mL respectively on sensitive (3D7) and multiresistant (Dd2) strains of P. falciparum. Of the sixteen compounds isolated, 3,3′,4-tri-O-methylellagic acid (4) exhibited the highest antiplasmodial activity against PfDd2 strains with an IC(50) value of 0.63 μg/mL. All extracts, fractions, and isolated compounds demonstrated no cytotoxicity against Raw cell lines with CC(50) > 250 μg/mL. In addition, the most active extract on both strains of P. falciparum was nontoxic in vivo, with a LD(50) greater than 2000 and 5000 mg/kg. A phytochemical investigation of the stem bark and leaves of D. edulis afforded sixteen compounds, including two xanthones (1–2), three ellagic acid derivatives (3–5), one phenolic compound (6), one depside (7), one triglyceride (8), one auranthiamide acetate (9), one gallic acid derivative (10), four triterpenoids (11–14), and two steroids (15–16). Compounds 1, 2, 5, 7, 8, and 9 were herein reported for the first time from the Burseraceae family. CONCLUSION: This work highlights the good in vitro antiplasmodial potency of the hydroethanolic extract of the leaves of this plant and that of two isolated constituents (3,3′,4-tri-O-methylellagic acid and ethylgallate) from the plant. These biological results support the use of D. edulis in traditional medicine against malaria. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-023-03957-2. BioMed Central 2023-06-27 /pmc/articles/PMC10303846/ /pubmed/37370061 http://dx.doi.org/10.1186/s12906-023-03957-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Dongmo, Kevine Johane Jumeta
Tali, Mariscal Brice Tchatat
Fongang, Yannick Stéphane Fotsing
Taguimjeu, Pierre Leonel K. Tafokeu
Kagho, Donald Ulrich Kenou
Bitchagno, Gabin Thierry
Lenta, Bruno Ndjakou
Boyom, Fabrice Fekam
Sewald, Norbert
Ngouela, Silvère Augustin
In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
title In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
title_full In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
title_fullStr In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
title_full_unstemmed In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
title_short In vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from Dacryodes edulis (Burseraceae)
title_sort in vitro antiplasmodial activity and toxicological profile of extracts, fractions and chemical constituents of leaves and stem bark from dacryodes edulis (burseraceae)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303846/
https://www.ncbi.nlm.nih.gov/pubmed/37370061
http://dx.doi.org/10.1186/s12906-023-03957-2
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