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Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan

Pseudomonas aeruginosa (P. aeruginosa) is known to be associated with resistance to practically all known antibiotics. This is a cross-sectional, descriptive, laboratory-based analytical study in which 200 P. aeruginosa clinical isolates were involved. The DNA of the most resistant isolate was extra...

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Autores principales: Hussain, Mohamed A., Mohamed, Malik Suliman, Altayb, Hisham N., Mohamed, Ahmed Osman, Ashour, Ahmed, Osman, Wadah, Sherif, Asmaa E., Ghazawi, Kholoud F., Miski, Samar F., Ibrahim, Sabrin R. M., Mohamed, Gamal A., Sindi, Ikhlas A., Alshamrani, Ahmad A., Elgaml, Abdelaziz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303892/
https://www.ncbi.nlm.nih.gov/pubmed/37374934
http://dx.doi.org/10.3390/microorganisms11061432
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author Hussain, Mohamed A.
Mohamed, Malik Suliman
Altayb, Hisham N.
Mohamed, Ahmed Osman
Ashour, Ahmed
Osman, Wadah
Sherif, Asmaa E.
Ghazawi, Kholoud F.
Miski, Samar F.
Ibrahim, Sabrin R. M.
Mohamed, Gamal A.
Sindi, Ikhlas A.
Alshamrani, Ahmad A.
Elgaml, Abdelaziz
author_facet Hussain, Mohamed A.
Mohamed, Malik Suliman
Altayb, Hisham N.
Mohamed, Ahmed Osman
Ashour, Ahmed
Osman, Wadah
Sherif, Asmaa E.
Ghazawi, Kholoud F.
Miski, Samar F.
Ibrahim, Sabrin R. M.
Mohamed, Gamal A.
Sindi, Ikhlas A.
Alshamrani, Ahmad A.
Elgaml, Abdelaziz
author_sort Hussain, Mohamed A.
collection PubMed
description Pseudomonas aeruginosa (P. aeruginosa) is known to be associated with resistance to practically all known antibiotics. This is a cross-sectional, descriptive, laboratory-based analytical study in which 200 P. aeruginosa clinical isolates were involved. The DNA of the most resistant isolate was extracted and its whole genome was sequenced, assembled, annotated, and announced, strain typing was ascribed, and it was subjected to comparative genomic analysis with two susceptible strains. The rate of resistance was 77.89%, 25.13%, 21.61%, 18.09%, 5.53%, and 4.52% for piperacillin, gentamicin, ciprofloxacin, ceftazidime, meropenem, and polymyxin B, respectively. Eighteen percent (36) of the tested isolates exhibited a MDR phenotype. The most MDR strain belonged to epidemic sequence type 235. Comparative genomic analysis of the MDR strain (GenBank: MVDK00000000) with two susceptible strains revealed that the core genes were shared by the three genomes but there were accessory genes that were strain-specific, and this MDR genome had a low CG% (64.6%) content. A prophage sequence and one plasmid were detected in the MDR genome, but amazingly, it contained no resistant genes for drugs with antipseudomonal activity and there was no resistant island. In addition, 67 resistant genes were detected, 19 of them were found only in the MDR genome and 48 genes were efflux pumps, and a novel deleterious point mutation (D87G) was detected in the gyrA gene. The novel deleterious mutation in the gyrA gene (D87G) is a known position behind quinolone resistance. Our findings emphasize the importance of adoption of infection control strategies to prevent dissemination of MDR isolates.
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spelling pubmed-103038922023-06-29 Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan Hussain, Mohamed A. Mohamed, Malik Suliman Altayb, Hisham N. Mohamed, Ahmed Osman Ashour, Ahmed Osman, Wadah Sherif, Asmaa E. Ghazawi, Kholoud F. Miski, Samar F. Ibrahim, Sabrin R. M. Mohamed, Gamal A. Sindi, Ikhlas A. Alshamrani, Ahmad A. Elgaml, Abdelaziz Microorganisms Article Pseudomonas aeruginosa (P. aeruginosa) is known to be associated with resistance to practically all known antibiotics. This is a cross-sectional, descriptive, laboratory-based analytical study in which 200 P. aeruginosa clinical isolates were involved. The DNA of the most resistant isolate was extracted and its whole genome was sequenced, assembled, annotated, and announced, strain typing was ascribed, and it was subjected to comparative genomic analysis with two susceptible strains. The rate of resistance was 77.89%, 25.13%, 21.61%, 18.09%, 5.53%, and 4.52% for piperacillin, gentamicin, ciprofloxacin, ceftazidime, meropenem, and polymyxin B, respectively. Eighteen percent (36) of the tested isolates exhibited a MDR phenotype. The most MDR strain belonged to epidemic sequence type 235. Comparative genomic analysis of the MDR strain (GenBank: MVDK00000000) with two susceptible strains revealed that the core genes were shared by the three genomes but there were accessory genes that were strain-specific, and this MDR genome had a low CG% (64.6%) content. A prophage sequence and one plasmid were detected in the MDR genome, but amazingly, it contained no resistant genes for drugs with antipseudomonal activity and there was no resistant island. In addition, 67 resistant genes were detected, 19 of them were found only in the MDR genome and 48 genes were efflux pumps, and a novel deleterious point mutation (D87G) was detected in the gyrA gene. The novel deleterious mutation in the gyrA gene (D87G) is a known position behind quinolone resistance. Our findings emphasize the importance of adoption of infection control strategies to prevent dissemination of MDR isolates. MDPI 2023-05-29 /pmc/articles/PMC10303892/ /pubmed/37374934 http://dx.doi.org/10.3390/microorganisms11061432 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hussain, Mohamed A.
Mohamed, Malik Suliman
Altayb, Hisham N.
Mohamed, Ahmed Osman
Ashour, Ahmed
Osman, Wadah
Sherif, Asmaa E.
Ghazawi, Kholoud F.
Miski, Samar F.
Ibrahim, Sabrin R. M.
Mohamed, Gamal A.
Sindi, Ikhlas A.
Alshamrani, Ahmad A.
Elgaml, Abdelaziz
Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan
title Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan
title_full Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan
title_fullStr Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan
title_full_unstemmed Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan
title_short Comparative Genomic Analysis of Multi-Drug Resistant Pseudomonas aeruginosa Sequence Type 235 Isolated from Sudan
title_sort comparative genomic analysis of multi-drug resistant pseudomonas aeruginosa sequence type 235 isolated from sudan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10303892/
https://www.ncbi.nlm.nih.gov/pubmed/37374934
http://dx.doi.org/10.3390/microorganisms11061432
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