Cargando…

Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi

Metal nanoparticles are assumed to be a new generation of biologically active materials. The integrations between more than one metal are synergetic multifunctional features. In the current study, trimetallic copper–selenium–zinc oxide nanoparticles (Tri-CSZ NPs) were successfully mycosynthesized us...

Descripción completa

Detalles Bibliográficos
Autores principales: Hashem, Amr H., Al-Askar, Abdulaziz A., Haponiuk, Józef, Abd-Elsalam, Kamel A., Hasanin, Mohamed S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304146/
https://www.ncbi.nlm.nih.gov/pubmed/37374882
http://dx.doi.org/10.3390/microorganisms11061380
_version_ 1785065438664720384
author Hashem, Amr H.
Al-Askar, Abdulaziz A.
Haponiuk, Józef
Abd-Elsalam, Kamel A.
Hasanin, Mohamed S.
author_facet Hashem, Amr H.
Al-Askar, Abdulaziz A.
Haponiuk, Józef
Abd-Elsalam, Kamel A.
Hasanin, Mohamed S.
author_sort Hashem, Amr H.
collection PubMed
description Metal nanoparticles are assumed to be a new generation of biologically active materials. The integrations between more than one metal are synergetic multifunctional features. In the current study, trimetallic copper–selenium–zinc oxide nanoparticles (Tri-CSZ NPs) were successfully mycosynthesized using Aspergillus niger through an ecofriendly method for the first time. The biosynthesis of the particles was characterized using physiochemical and topographical analysis. The physiochemical analysis included Fourier transform infrared spectroscopy (FTIR), which affirmed that the biosynthesis of Tri-CSZ NPs relies on the functional groups of fungal filtrates. Additionally, the UV–visible and X-ray diffraction patterns were proposed for the formation of Tri-CSZ NPs; moreover, topography analysis confirmed that the micromorphology of the nanoparticles were similar to a stick, with ends having a tetragonal pyramid shape, and with an average nanosize of about 26.3 ± 5.4 nm. Cytotoxicity results reveled that the Tri-CSZ NPs have no cytotoxicity on the human normal cell line Wi 38 at low concentrations, where the IC50 was 521 µg/mL. Furthermore, the antifungal activity of the Tri-CSZ NPs was evaluated. The antifungal results revealed that the Tri-CSZ NPs have promising antifungal activity against Mucor racemosus, Rhizopus microsporus, Lichtheimia corymbifera, and Syncephalastrum racemosum, where the minimum inhibitory concentrations (MICs) were 1.95, 7.81, 62.5, and 3.9 µg/mL, and the minimum fungicidal concentrations (MFCs) were 250, 62.5, 125, and 1000 µg/mL, respectively. In conclusion, Tri-CSZ NPs were successfully mycosynthesized using A. niger, which have a promising antifungal activity against fungi causing mucormycosis.
format Online
Article
Text
id pubmed-10304146
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103041462023-06-29 Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi Hashem, Amr H. Al-Askar, Abdulaziz A. Haponiuk, Józef Abd-Elsalam, Kamel A. Hasanin, Mohamed S. Microorganisms Article Metal nanoparticles are assumed to be a new generation of biologically active materials. The integrations between more than one metal are synergetic multifunctional features. In the current study, trimetallic copper–selenium–zinc oxide nanoparticles (Tri-CSZ NPs) were successfully mycosynthesized using Aspergillus niger through an ecofriendly method for the first time. The biosynthesis of the particles was characterized using physiochemical and topographical analysis. The physiochemical analysis included Fourier transform infrared spectroscopy (FTIR), which affirmed that the biosynthesis of Tri-CSZ NPs relies on the functional groups of fungal filtrates. Additionally, the UV–visible and X-ray diffraction patterns were proposed for the formation of Tri-CSZ NPs; moreover, topography analysis confirmed that the micromorphology of the nanoparticles were similar to a stick, with ends having a tetragonal pyramid shape, and with an average nanosize of about 26.3 ± 5.4 nm. Cytotoxicity results reveled that the Tri-CSZ NPs have no cytotoxicity on the human normal cell line Wi 38 at low concentrations, where the IC50 was 521 µg/mL. Furthermore, the antifungal activity of the Tri-CSZ NPs was evaluated. The antifungal results revealed that the Tri-CSZ NPs have promising antifungal activity against Mucor racemosus, Rhizopus microsporus, Lichtheimia corymbifera, and Syncephalastrum racemosum, where the minimum inhibitory concentrations (MICs) were 1.95, 7.81, 62.5, and 3.9 µg/mL, and the minimum fungicidal concentrations (MFCs) were 250, 62.5, 125, and 1000 µg/mL, respectively. In conclusion, Tri-CSZ NPs were successfully mycosynthesized using A. niger, which have a promising antifungal activity against fungi causing mucormycosis. MDPI 2023-05-24 /pmc/articles/PMC10304146/ /pubmed/37374882 http://dx.doi.org/10.3390/microorganisms11061380 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hashem, Amr H.
Al-Askar, Abdulaziz A.
Haponiuk, Józef
Abd-Elsalam, Kamel A.
Hasanin, Mohamed S.
Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi
title Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi
title_full Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi
title_fullStr Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi
title_full_unstemmed Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi
title_short Biosynthesis, Characterization, and Antifungal Activity of Novel Trimetallic Copper Oxide–Selenium–Zinc Oxide Nanoparticles against Some Mucorales Fungi
title_sort biosynthesis, characterization, and antifungal activity of novel trimetallic copper oxide–selenium–zinc oxide nanoparticles against some mucorales fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304146/
https://www.ncbi.nlm.nih.gov/pubmed/37374882
http://dx.doi.org/10.3390/microorganisms11061380
work_keys_str_mv AT hashemamrh biosynthesischaracterizationandantifungalactivityofnoveltrimetalliccopperoxideseleniumzincoxidenanoparticlesagainstsomemucoralesfungi
AT alaskarabdulaziza biosynthesischaracterizationandantifungalactivityofnoveltrimetalliccopperoxideseleniumzincoxidenanoparticlesagainstsomemucoralesfungi
AT haponiukjozef biosynthesischaracterizationandantifungalactivityofnoveltrimetalliccopperoxideseleniumzincoxidenanoparticlesagainstsomemucoralesfungi
AT abdelsalamkamela biosynthesischaracterizationandantifungalactivityofnoveltrimetalliccopperoxideseleniumzincoxidenanoparticlesagainstsomemucoralesfungi
AT hasaninmohameds biosynthesischaracterizationandantifungalactivityofnoveltrimetalliccopperoxideseleniumzincoxidenanoparticlesagainstsomemucoralesfungi