Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)

Oilseed rape (Brassica napus L.) is an important cash crop, but transgenic oilseed rape has not been grown on a commercial scale in China. It is necessary to analyze the characteristics of transgenic oilseed rape before commercial cultivation. In our study, differential expression of total protein f...

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Autores principales: Guan, Zheng-Jun, Zheng, Min, Tang, Zhi-Xi, Wei, Wei, Stewart, C. Neal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304370/
https://www.ncbi.nlm.nih.gov/pubmed/37375944
http://dx.doi.org/10.3390/plants12122319
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author Guan, Zheng-Jun
Zheng, Min
Tang, Zhi-Xi
Wei, Wei
Stewart, C. Neal
author_facet Guan, Zheng-Jun
Zheng, Min
Tang, Zhi-Xi
Wei, Wei
Stewart, C. Neal
author_sort Guan, Zheng-Jun
collection PubMed
description Oilseed rape (Brassica napus L.) is an important cash crop, but transgenic oilseed rape has not been grown on a commercial scale in China. It is necessary to analyze the characteristics of transgenic oilseed rape before commercial cultivation. In our study, differential expression of total protein from the leaves in two transgenic lines of oilseed rape expressing foreign Bt Cry1Ac insecticidal toxin and their non-transgenic parent plant was analyzed using a proteomic approach. Only shared changes in both of the two transgenic lines were calculated. Fourteen differential protein spots were analyzed and identified, namely, eleven upregulated expressed protein spots and three downregulated protein spots. These proteins are involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. The changes of these protein spots in transgenic oilseed rape may be attributable to the insertion of the foreign transgenes. However, the transgenic manipulation might not necessarily cause significant change in proteomes of the oilseed rape.
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spelling pubmed-103043702023-06-29 Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.) Guan, Zheng-Jun Zheng, Min Tang, Zhi-Xi Wei, Wei Stewart, C. Neal Plants (Basel) Article Oilseed rape (Brassica napus L.) is an important cash crop, but transgenic oilseed rape has not been grown on a commercial scale in China. It is necessary to analyze the characteristics of transgenic oilseed rape before commercial cultivation. In our study, differential expression of total protein from the leaves in two transgenic lines of oilseed rape expressing foreign Bt Cry1Ac insecticidal toxin and their non-transgenic parent plant was analyzed using a proteomic approach. Only shared changes in both of the two transgenic lines were calculated. Fourteen differential protein spots were analyzed and identified, namely, eleven upregulated expressed protein spots and three downregulated protein spots. These proteins are involved in photosynthesis, transporter function, metabolism, protein synthesis, and cell growth and differentiation. The changes of these protein spots in transgenic oilseed rape may be attributable to the insertion of the foreign transgenes. However, the transgenic manipulation might not necessarily cause significant change in proteomes of the oilseed rape. MDPI 2023-06-15 /pmc/articles/PMC10304370/ /pubmed/37375944 http://dx.doi.org/10.3390/plants12122319 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guan, Zheng-Jun
Zheng, Min
Tang, Zhi-Xi
Wei, Wei
Stewart, C. Neal
Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)
title Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)
title_full Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)
title_fullStr Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)
title_full_unstemmed Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)
title_short Proteomic Analysis of Bt cry1Ac Transgenic Oilseed Rape (Brassica napus L.)
title_sort proteomic analysis of bt cry1ac transgenic oilseed rape (brassica napus l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304370/
https://www.ncbi.nlm.nih.gov/pubmed/37375944
http://dx.doi.org/10.3390/plants12122319
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