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The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913
Swarming regulation is complicated in flagellated bacteria, especially those possessing dual flagellar systems. It remains unclear whether and how the movement of the constitutive polar flagellum is regulated during swarming motility of these bacteria. Here, we report the downregulation of polar fla...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304486/ https://www.ncbi.nlm.nih.gov/pubmed/37375068 http://dx.doi.org/10.3390/microorganisms11061566 |
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author | Sheng, Qi Liu, Ang Yang, Peiling Chen, Zhuowei Wang, Peng Sun, Haining Li, Chunyang McMinn, Andrew Chen, Yin Zhang, Yuzhong Su, Hainan Chen, Xiulan Zhang, Yuqiang |
author_facet | Sheng, Qi Liu, Ang Yang, Peiling Chen, Zhuowei Wang, Peng Sun, Haining Li, Chunyang McMinn, Andrew Chen, Yin Zhang, Yuzhong Su, Hainan Chen, Xiulan Zhang, Yuqiang |
author_sort | Sheng, Qi |
collection | PubMed |
description | Swarming regulation is complicated in flagellated bacteria, especially those possessing dual flagellar systems. It remains unclear whether and how the movement of the constitutive polar flagellum is regulated during swarming motility of these bacteria. Here, we report the downregulation of polar flagellar motility by the c-di-GMP effector FilZ in the marine sedimentary bacterium Pseudoalteromonas sp. SM9913. Strain SM9913 possesses two flagellar systems, and filZ is located in the lateral flagellar gene cluster. The function of FilZ is negatively controlled by intracellular c-di-GMP. Swarming in strain SM9913 consists of three periods. Deletion and overexpression of filZ revealed that, during the period when strain SM9913 expands quickly, FilZ facilitates swarming. In vitro pull-down and bacterial two-hybrid assays suggested that, in the absence of c-di-GMP, FilZ interacts with the CheW homolog A2230, which may be involved in the chemotactic signal transduction pathway to the polar flagellar motor protein FliM(p), to interfere with polar flagellar motility. When bound to c-di-GMP, FilZ loses its ability to interact with A2230. Bioinformatic investigation indicated that filZ-like genes are present in many bacteria with dual flagellar systems. Our findings demonstrate a novel mode of regulation of bacterial swarming motility. |
format | Online Article Text |
id | pubmed-10304486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103044862023-06-29 The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 Sheng, Qi Liu, Ang Yang, Peiling Chen, Zhuowei Wang, Peng Sun, Haining Li, Chunyang McMinn, Andrew Chen, Yin Zhang, Yuzhong Su, Hainan Chen, Xiulan Zhang, Yuqiang Microorganisms Article Swarming regulation is complicated in flagellated bacteria, especially those possessing dual flagellar systems. It remains unclear whether and how the movement of the constitutive polar flagellum is regulated during swarming motility of these bacteria. Here, we report the downregulation of polar flagellar motility by the c-di-GMP effector FilZ in the marine sedimentary bacterium Pseudoalteromonas sp. SM9913. Strain SM9913 possesses two flagellar systems, and filZ is located in the lateral flagellar gene cluster. The function of FilZ is negatively controlled by intracellular c-di-GMP. Swarming in strain SM9913 consists of three periods. Deletion and overexpression of filZ revealed that, during the period when strain SM9913 expands quickly, FilZ facilitates swarming. In vitro pull-down and bacterial two-hybrid assays suggested that, in the absence of c-di-GMP, FilZ interacts with the CheW homolog A2230, which may be involved in the chemotactic signal transduction pathway to the polar flagellar motor protein FliM(p), to interfere with polar flagellar motility. When bound to c-di-GMP, FilZ loses its ability to interact with A2230. Bioinformatic investigation indicated that filZ-like genes are present in many bacteria with dual flagellar systems. Our findings demonstrate a novel mode of regulation of bacterial swarming motility. MDPI 2023-06-13 /pmc/articles/PMC10304486/ /pubmed/37375068 http://dx.doi.org/10.3390/microorganisms11061566 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sheng, Qi Liu, Ang Yang, Peiling Chen, Zhuowei Wang, Peng Sun, Haining Li, Chunyang McMinn, Andrew Chen, Yin Zhang, Yuzhong Su, Hainan Chen, Xiulan Zhang, Yuqiang The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 |
title | The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 |
title_full | The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 |
title_fullStr | The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 |
title_full_unstemmed | The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 |
title_short | The FilZ Protein Contains a Single PilZ Domain and Facilitates the Swarming Motility of Pseudoalteromonas sp. SM9913 |
title_sort | filz protein contains a single pilz domain and facilitates the swarming motility of pseudoalteromonas sp. sm9913 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304486/ https://www.ncbi.nlm.nih.gov/pubmed/37375068 http://dx.doi.org/10.3390/microorganisms11061566 |
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