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Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection

Middle East respiratory syndrome coronavirus (MERS-CoV) is associated with significant morbidity and mortality due to intense pulmonary inflammation. Enhanced chemokine-mediated leukocyte infiltration in lungs has been linked with unfavorable outcomes with respect to the disease. This cross-sectiona...

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Autores principales: Alhetheel, Abdulkarim, Albarrag, Ahmed, Shakoor, Zahid, Somily, Ali, Barry, Mazin, Altalhi, Haifa, Bakhrebah, Muhammed, Nassar, Majed, Alfageeh, Mohamed, Assiri, Ayed, Alfaraj, Sarah, Memish, Ziad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304594/
https://www.ncbi.nlm.nih.gov/pubmed/37376437
http://dx.doi.org/10.3390/vaccines11061048
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author Alhetheel, Abdulkarim
Albarrag, Ahmed
Shakoor, Zahid
Somily, Ali
Barry, Mazin
Altalhi, Haifa
Bakhrebah, Muhammed
Nassar, Majed
Alfageeh, Mohamed
Assiri, Ayed
Alfaraj, Sarah
Memish, Ziad
author_facet Alhetheel, Abdulkarim
Albarrag, Ahmed
Shakoor, Zahid
Somily, Ali
Barry, Mazin
Altalhi, Haifa
Bakhrebah, Muhammed
Nassar, Majed
Alfageeh, Mohamed
Assiri, Ayed
Alfaraj, Sarah
Memish, Ziad
author_sort Alhetheel, Abdulkarim
collection PubMed
description Middle East respiratory syndrome coronavirus (MERS-CoV) is associated with significant morbidity and mortality due to intense pulmonary inflammation. Enhanced chemokine-mediated leukocyte infiltration in lungs has been linked with unfavorable outcomes with respect to the disease. This cross-sectional study assessed the levels of chemokines among 46 MERS-CoV-infected patients (19 asymptomatic and 27 symptomatic) and 52 healthy controls using a customized Luminex human chemokine magnetic multiplex panel. The plasma levels of interferon-inducible protein (IP)-10 (568.5 ± 114.7 vs. 55.19 ± 5.85 pg/mL; p < 0.0001), macrophage inflammatory protein (MIP)-1 alpha (MIP-1A) (30.78 ± 2.81 vs. 18.16 ± 0.91 pg/mL; p < 0.0001), MIP-1B (36.63 ± 4.25 vs. 25.26 ± 1.51 pg/mL; p < 0.003), monocyte chemoattractant protein (MCP)-1 (1267 ± 309.5 vs. 390.0 ± 35.51 pg/mL; p < 0.0002), and monokine-induced gamma interferon (MIG) (28.96 ± 3.93 vs. 16.29 ± 1.69 pg/mL; p < 0.001), interleukin (IL)-8 (147.9 ± 21.57 vs. 84.63 ± 10.62 pg/mL; p < 0.004) were significantly higher in symptomatic patients than healthy controls. Likewise, the levels of IP-10 (247.6 ± 80.09 vs. 55.19 ± 5.85 pg/mL; p < 0.0002) and MCP-1 (650.7 ± 149 pg/mL vs. 390 ± 35.51 pg/mL; p < 0.02) were also significantly higher in asymptomatic patients compared to healthy controls. However, no differences were observed in the plasma levels of MIP-1A, MIP-1B, MIG, and IL-8 between asymptomatic patients and uninfected controls. Conversely, the mean plasma levels of regulated on activation normal T cell expressed and secreted (RANTES) (3039 ± 301.0 vs. 4390 ± 223 pg/mL; p < 0.001) and eotaxin (176.9 ± 30.20 vs. 296.2 ± 28.11 pg/mL; p < 0.01) were significantly lower in symptomatic MERS-CoV-infected patients compared to healthy controls. Likewise, the levels of eotaxin (162.7 ± 21.60 vs. 296.2 ± 28.11 pg/mL; p < 0.01) were also significantly lower in asymptomatic patients. Interestingly, the level of MCP-1 (2139 ± 548.2 vs. 776.5 ± 165.3 pg/mL; p < 0.004) was significantly higher in deceased symptomatic patients compared to recovered symptomatic patients. MCP-1 was the only chemokine associated with a higher risk of mortality. Symptomatic MERS-CoV-infected patients had a significant elevation of plasma chemokines and elevated MCP-1 levels were found to be associated with fatal outcomes.
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spelling pubmed-103045942023-06-29 Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection Alhetheel, Abdulkarim Albarrag, Ahmed Shakoor, Zahid Somily, Ali Barry, Mazin Altalhi, Haifa Bakhrebah, Muhammed Nassar, Majed Alfageeh, Mohamed Assiri, Ayed Alfaraj, Sarah Memish, Ziad Vaccines (Basel) Article Middle East respiratory syndrome coronavirus (MERS-CoV) is associated with significant morbidity and mortality due to intense pulmonary inflammation. Enhanced chemokine-mediated leukocyte infiltration in lungs has been linked with unfavorable outcomes with respect to the disease. This cross-sectional study assessed the levels of chemokines among 46 MERS-CoV-infected patients (19 asymptomatic and 27 symptomatic) and 52 healthy controls using a customized Luminex human chemokine magnetic multiplex panel. The plasma levels of interferon-inducible protein (IP)-10 (568.5 ± 114.7 vs. 55.19 ± 5.85 pg/mL; p < 0.0001), macrophage inflammatory protein (MIP)-1 alpha (MIP-1A) (30.78 ± 2.81 vs. 18.16 ± 0.91 pg/mL; p < 0.0001), MIP-1B (36.63 ± 4.25 vs. 25.26 ± 1.51 pg/mL; p < 0.003), monocyte chemoattractant protein (MCP)-1 (1267 ± 309.5 vs. 390.0 ± 35.51 pg/mL; p < 0.0002), and monokine-induced gamma interferon (MIG) (28.96 ± 3.93 vs. 16.29 ± 1.69 pg/mL; p < 0.001), interleukin (IL)-8 (147.9 ± 21.57 vs. 84.63 ± 10.62 pg/mL; p < 0.004) were significantly higher in symptomatic patients than healthy controls. Likewise, the levels of IP-10 (247.6 ± 80.09 vs. 55.19 ± 5.85 pg/mL; p < 0.0002) and MCP-1 (650.7 ± 149 pg/mL vs. 390 ± 35.51 pg/mL; p < 0.02) were also significantly higher in asymptomatic patients compared to healthy controls. However, no differences were observed in the plasma levels of MIP-1A, MIP-1B, MIG, and IL-8 between asymptomatic patients and uninfected controls. Conversely, the mean plasma levels of regulated on activation normal T cell expressed and secreted (RANTES) (3039 ± 301.0 vs. 4390 ± 223 pg/mL; p < 0.001) and eotaxin (176.9 ± 30.20 vs. 296.2 ± 28.11 pg/mL; p < 0.01) were significantly lower in symptomatic MERS-CoV-infected patients compared to healthy controls. Likewise, the levels of eotaxin (162.7 ± 21.60 vs. 296.2 ± 28.11 pg/mL; p < 0.01) were also significantly lower in asymptomatic patients. Interestingly, the level of MCP-1 (2139 ± 548.2 vs. 776.5 ± 165.3 pg/mL; p < 0.004) was significantly higher in deceased symptomatic patients compared to recovered symptomatic patients. MCP-1 was the only chemokine associated with a higher risk of mortality. Symptomatic MERS-CoV-infected patients had a significant elevation of plasma chemokines and elevated MCP-1 levels were found to be associated with fatal outcomes. MDPI 2023-05-31 /pmc/articles/PMC10304594/ /pubmed/37376437 http://dx.doi.org/10.3390/vaccines11061048 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alhetheel, Abdulkarim
Albarrag, Ahmed
Shakoor, Zahid
Somily, Ali
Barry, Mazin
Altalhi, Haifa
Bakhrebah, Muhammed
Nassar, Majed
Alfageeh, Mohamed
Assiri, Ayed
Alfaraj, Sarah
Memish, Ziad
Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection
title Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection
title_full Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection
title_fullStr Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection
title_full_unstemmed Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection
title_short Chemokine Levels among Patients with Middle East Respiratory Syndrome Coronavirus Infection
title_sort chemokine levels among patients with middle east respiratory syndrome coronavirus infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304594/
https://www.ncbi.nlm.nih.gov/pubmed/37376437
http://dx.doi.org/10.3390/vaccines11061048
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