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Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species

The genus Pleuronema Dujardin, 1841, with nearly 40 morphospecies, is one of the largest genera in the well-known subclass Scuticociliatia. In the present study, two Pleuronema species were collected from subtropical coastal waters of the East China Sea. The morphology and molecular phylogeny were i...

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Autores principales: Zhang, Hui, Zhao, Xuetong, Ye, Tingting, Wu, Zehao, Wu, Fan, Chen, Xiangrui, Liu, Mingjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304819/
https://www.ncbi.nlm.nih.gov/pubmed/37374924
http://dx.doi.org/10.3390/microorganisms11061422
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author Zhang, Hui
Zhao, Xuetong
Ye, Tingting
Wu, Zehao
Wu, Fan
Chen, Xiangrui
Liu, Mingjian
author_facet Zhang, Hui
Zhao, Xuetong
Ye, Tingting
Wu, Zehao
Wu, Fan
Chen, Xiangrui
Liu, Mingjian
author_sort Zhang, Hui
collection PubMed
description The genus Pleuronema Dujardin, 1841, with nearly 40 morphospecies, is one of the largest genera in the well-known subclass Scuticociliatia. In the present study, two Pleuronema species were collected from subtropical coastal waters of the East China Sea. The morphology and molecular phylogeny were investigated using modern standard methods. Pleuronema ningboensis n. sp. is mainly characterized by an elliptical body in outline with the right ventrolateral side straight, 16–22 somatic kineties, 3–5 preoral kineties, and the posterior end of the membranelle 2a hook-like. An improved diagnosis of Pleuronema orientale Pan et al., 2015 was provided: body size in vivo usually 90–135 × 45–85 μm, right ventrolateral side convex, 36–51 somatic kineties, 1–5 preoral kineties, one to three spherical macronuclei, membranelle 2a arranged in a zig-zag pattern in middle portion, posterior region hook-like, both membranelle 1 and membranelle 3 composed of three rows of basal bodies. The small subunit ribosomal DNA (SSU rDNA) of two species is sequenced, and their molecular phylogeny is analyzed. The new species Pleuronema ningboensis n. sp. clusters with P. grolierei KF840519, P. setigerum JX310015, P. paucisaetosum KF206430, and P. cf. setigerum KF848875, basically in accord with the morphological characteristics.
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spelling pubmed-103048192023-06-29 Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species Zhang, Hui Zhao, Xuetong Ye, Tingting Wu, Zehao Wu, Fan Chen, Xiangrui Liu, Mingjian Microorganisms Article The genus Pleuronema Dujardin, 1841, with nearly 40 morphospecies, is one of the largest genera in the well-known subclass Scuticociliatia. In the present study, two Pleuronema species were collected from subtropical coastal waters of the East China Sea. The morphology and molecular phylogeny were investigated using modern standard methods. Pleuronema ningboensis n. sp. is mainly characterized by an elliptical body in outline with the right ventrolateral side straight, 16–22 somatic kineties, 3–5 preoral kineties, and the posterior end of the membranelle 2a hook-like. An improved diagnosis of Pleuronema orientale Pan et al., 2015 was provided: body size in vivo usually 90–135 × 45–85 μm, right ventrolateral side convex, 36–51 somatic kineties, 1–5 preoral kineties, one to three spherical macronuclei, membranelle 2a arranged in a zig-zag pattern in middle portion, posterior region hook-like, both membranelle 1 and membranelle 3 composed of three rows of basal bodies. The small subunit ribosomal DNA (SSU rDNA) of two species is sequenced, and their molecular phylogeny is analyzed. The new species Pleuronema ningboensis n. sp. clusters with P. grolierei KF840519, P. setigerum JX310015, P. paucisaetosum KF206430, and P. cf. setigerum KF848875, basically in accord with the morphological characteristics. MDPI 2023-05-27 /pmc/articles/PMC10304819/ /pubmed/37374924 http://dx.doi.org/10.3390/microorganisms11061422 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Hui
Zhao, Xuetong
Ye, Tingting
Wu, Zehao
Wu, Fan
Chen, Xiangrui
Liu, Mingjian
Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species
title Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species
title_full Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species
title_fullStr Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species
title_full_unstemmed Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species
title_short Taxonomic and Phylogenetic Studies of Two Brackish Pleuronema Species (Protista, Ciliophora, Scuticociliatia) from Subtropical Coastal Waters of China, with Report of a New Species
title_sort taxonomic and phylogenetic studies of two brackish pleuronema species (protista, ciliophora, scuticociliatia) from subtropical coastal waters of china, with report of a new species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304819/
https://www.ncbi.nlm.nih.gov/pubmed/37374924
http://dx.doi.org/10.3390/microorganisms11061422
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