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Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor

Crocins are glycosylated apocarotenoids with strong coloring power and anti-oxidant, anticancer, and neuro-protective properties. We previously dissected the saffron crocin biosynthesis pathway, and demonstrated that the CsCCD2 enzyme, catalyzing the carotenoid cleavage step, shows a strong preferen...

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Autores principales: Demurtas, Olivia Costantina, Sulli, Maria, Ferrante, Paola, Mini, Paola, Martí, Maricarmen, Aragonés, Verónica, Daròs, José-Antonio, Giuliano, Giovanni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305034/
https://www.ncbi.nlm.nih.gov/pubmed/37367887
http://dx.doi.org/10.3390/metabo13060729
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author Demurtas, Olivia Costantina
Sulli, Maria
Ferrante, Paola
Mini, Paola
Martí, Maricarmen
Aragonés, Verónica
Daròs, José-Antonio
Giuliano, Giovanni
author_facet Demurtas, Olivia Costantina
Sulli, Maria
Ferrante, Paola
Mini, Paola
Martí, Maricarmen
Aragonés, Verónica
Daròs, José-Antonio
Giuliano, Giovanni
author_sort Demurtas, Olivia Costantina
collection PubMed
description Crocins are glycosylated apocarotenoids with strong coloring power and anti-oxidant, anticancer, and neuro-protective properties. We previously dissected the saffron crocin biosynthesis pathway, and demonstrated that the CsCCD2 enzyme, catalyzing the carotenoid cleavage step, shows a strong preference for the xanthophyll zeaxanthin in vitro and in bacterio. In order to investigate substrate specificity in planta and to establish a plant-based bio-factory system for crocin production, we compared wild-type Nicotiana benthamiana plants, accumulating various xanthophylls together with α- and β-carotene, with genome-edited lines, in which all the xanthophylls normally accumulated in leaves were replaced by a single xanthophyll, zeaxanthin. These plants were used as chassis for the production in leaves of saffron apocarotenoids (crocins, picrocrocin) using two transient expression methods to overexpress CsCCD2: agroinfiltration and inoculation with a viral vector derived from tobacco etch virus (TEV). The results indicated the superior performance of the zeaxanthin-accumulating line and of the use of the viral vector to express CsCCD2. The results also suggested a relaxed substrate specificity of CsCCD2 in planta, cleaving additional carotenoid substrates.
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spelling pubmed-103050342023-06-29 Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor Demurtas, Olivia Costantina Sulli, Maria Ferrante, Paola Mini, Paola Martí, Maricarmen Aragonés, Verónica Daròs, José-Antonio Giuliano, Giovanni Metabolites Article Crocins are glycosylated apocarotenoids with strong coloring power and anti-oxidant, anticancer, and neuro-protective properties. We previously dissected the saffron crocin biosynthesis pathway, and demonstrated that the CsCCD2 enzyme, catalyzing the carotenoid cleavage step, shows a strong preference for the xanthophyll zeaxanthin in vitro and in bacterio. In order to investigate substrate specificity in planta and to establish a plant-based bio-factory system for crocin production, we compared wild-type Nicotiana benthamiana plants, accumulating various xanthophylls together with α- and β-carotene, with genome-edited lines, in which all the xanthophylls normally accumulated in leaves were replaced by a single xanthophyll, zeaxanthin. These plants were used as chassis for the production in leaves of saffron apocarotenoids (crocins, picrocrocin) using two transient expression methods to overexpress CsCCD2: agroinfiltration and inoculation with a viral vector derived from tobacco etch virus (TEV). The results indicated the superior performance of the zeaxanthin-accumulating line and of the use of the viral vector to express CsCCD2. The results also suggested a relaxed substrate specificity of CsCCD2 in planta, cleaving additional carotenoid substrates. MDPI 2023-06-06 /pmc/articles/PMC10305034/ /pubmed/37367887 http://dx.doi.org/10.3390/metabo13060729 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Demurtas, Olivia Costantina
Sulli, Maria
Ferrante, Paola
Mini, Paola
Martí, Maricarmen
Aragonés, Verónica
Daròs, José-Antonio
Giuliano, Giovanni
Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor
title Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor
title_full Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor
title_fullStr Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor
title_full_unstemmed Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor
title_short Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor
title_sort production of saffron apocarotenoids in nicotiana benthamiana plants genome-edited to accumulate zeaxanthin precursor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305034/
https://www.ncbi.nlm.nih.gov/pubmed/37367887
http://dx.doi.org/10.3390/metabo13060729
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