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A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy

Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent...

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Detalles Bibliográficos
Autores principales: Weng, Xiaoxing, Li, Chen, Chen, Changqing, Wang, Gang, Xia, Chenghao, Zheng, Lianyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305216/
https://www.ncbi.nlm.nih.gov/pubmed/37374703
http://dx.doi.org/10.3390/mi14061118
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author Weng, Xiaoxing
Li, Chen
Chen, Changqing
Wang, Gang
Xia, Chenghao
Zheng, Lianyou
author_facet Weng, Xiaoxing
Li, Chen
Chen, Changqing
Wang, Gang
Xia, Chenghao
Zheng, Lianyou
author_sort Weng, Xiaoxing
collection PubMed
description Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detection device. Based on this model, a tobacco ringspot virus detection device was designed by using an AD5933 impedance detection chip. A comprehensive test study was carried out on the developed tobacco ringspot virus detection device through various testing methods, which verified the feasibility of the tobacco ringspot virus detection device and provided technical support for the field detection of pathogenic microorganisms.
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spelling pubmed-103052162023-06-29 A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy Weng, Xiaoxing Li, Chen Chen, Changqing Wang, Gang Xia, Chenghao Zheng, Lianyou Micromachines (Basel) Article Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detection device. Based on this model, a tobacco ringspot virus detection device was designed by using an AD5933 impedance detection chip. A comprehensive test study was carried out on the developed tobacco ringspot virus detection device through various testing methods, which verified the feasibility of the tobacco ringspot virus detection device and provided technical support for the field detection of pathogenic microorganisms. MDPI 2023-05-26 /pmc/articles/PMC10305216/ /pubmed/37374703 http://dx.doi.org/10.3390/mi14061118 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weng, Xiaoxing
Li, Chen
Chen, Changqing
Wang, Gang
Xia, Chenghao
Zheng, Lianyou
A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
title A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
title_full A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
title_fullStr A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
title_full_unstemmed A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
title_short A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
title_sort microfluidic device for tobacco ringspot virus detection by electrochemical impedance spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305216/
https://www.ncbi.nlm.nih.gov/pubmed/37374703
http://dx.doi.org/10.3390/mi14061118
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