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A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy
Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305216/ https://www.ncbi.nlm.nih.gov/pubmed/37374703 http://dx.doi.org/10.3390/mi14061118 |
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author | Weng, Xiaoxing Li, Chen Chen, Changqing Wang, Gang Xia, Chenghao Zheng, Lianyou |
author_facet | Weng, Xiaoxing Li, Chen Chen, Changqing Wang, Gang Xia, Chenghao Zheng, Lianyou |
author_sort | Weng, Xiaoxing |
collection | PubMed |
description | Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detection device. Based on this model, a tobacco ringspot virus detection device was designed by using an AD5933 impedance detection chip. A comprehensive test study was carried out on the developed tobacco ringspot virus detection device through various testing methods, which verified the feasibility of the tobacco ringspot virus detection device and provided technical support for the field detection of pathogenic microorganisms. |
format | Online Article Text |
id | pubmed-10305216 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103052162023-06-29 A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy Weng, Xiaoxing Li, Chen Chen, Changqing Wang, Gang Xia, Chenghao Zheng, Lianyou Micromachines (Basel) Article Aiming at the problem of how to achieve the rapid detection of pathogenic microorganisms, this paper takes tobacco ringspot virus as the detection object, designs the impedance detection and analysis platform of tobacco ringspot virus based on microfluidic impedance method, establishes an equivalent circuit model to analyze the experimental results, and determines the optimal detection frequency of tobacco ringspot virus detection. Based on this frequency, an impedance–concentration regression model was established for the detection of tobacco ringspot virus in a tobacco ringspot virus detection device. Based on this model, a tobacco ringspot virus detection device was designed by using an AD5933 impedance detection chip. A comprehensive test study was carried out on the developed tobacco ringspot virus detection device through various testing methods, which verified the feasibility of the tobacco ringspot virus detection device and provided technical support for the field detection of pathogenic microorganisms. MDPI 2023-05-26 /pmc/articles/PMC10305216/ /pubmed/37374703 http://dx.doi.org/10.3390/mi14061118 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Weng, Xiaoxing Li, Chen Chen, Changqing Wang, Gang Xia, Chenghao Zheng, Lianyou A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
title | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
title_full | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
title_fullStr | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
title_full_unstemmed | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
title_short | A Microfluidic Device for Tobacco Ringspot Virus Detection by Electrochemical Impedance Spectroscopy |
title_sort | microfluidic device for tobacco ringspot virus detection by electrochemical impedance spectroscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305216/ https://www.ncbi.nlm.nih.gov/pubmed/37374703 http://dx.doi.org/10.3390/mi14061118 |
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