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Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery

The effect of carbon dots (CDs) on a model blayer membrane was studied as a means of comprehending their ability to affect cell membranes. Initially, the interaction of N-doped carbon dots with a biophysical liposomal cell membrane model was investigated by dynamic light scattering, z-potential, tem...

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Autores principales: Mavroidi, Barbara, Kaminari, Archontia, Sakellis, Elias, Sideratou, Zili, Tsiourvas, Dimitris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305404/
https://www.ncbi.nlm.nih.gov/pubmed/37375780
http://dx.doi.org/10.3390/ph16060833
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author Mavroidi, Barbara
Kaminari, Archontia
Sakellis, Elias
Sideratou, Zili
Tsiourvas, Dimitris
author_facet Mavroidi, Barbara
Kaminari, Archontia
Sakellis, Elias
Sideratou, Zili
Tsiourvas, Dimitris
author_sort Mavroidi, Barbara
collection PubMed
description The effect of carbon dots (CDs) on a model blayer membrane was studied as a means of comprehending their ability to affect cell membranes. Initially, the interaction of N-doped carbon dots with a biophysical liposomal cell membrane model was investigated by dynamic light scattering, z-potential, temperature-modulated differential scanning calorimetry, and membrane permeability. CDs with a slightly positive charge interacted with the surface of the negative-charged liposomes and evidence indicated that the association of CDs with the membrane affects the structural and thermodynamic properties of the bilayer; most importantly, it enhances the bilayer’s permeability against doxorubicin, a well-known anticancer drug. The results, like those of similar studies that surveyed the interaction of proteins with lipid membranes, suggest that carbon dots are partially embedded in the bilayer. In vitro experiments employing breast cancer cell lines and human healthy dermal cells corroborated the findings, as it was shown that the presence of CDs in the culture medium selectively enhanced cell internalization of doxorubicin and, subsequently, increased its cytotoxicity, acting as a drug sensitizer.
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spelling pubmed-103054042023-06-29 Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery Mavroidi, Barbara Kaminari, Archontia Sakellis, Elias Sideratou, Zili Tsiourvas, Dimitris Pharmaceuticals (Basel) Article The effect of carbon dots (CDs) on a model blayer membrane was studied as a means of comprehending their ability to affect cell membranes. Initially, the interaction of N-doped carbon dots with a biophysical liposomal cell membrane model was investigated by dynamic light scattering, z-potential, temperature-modulated differential scanning calorimetry, and membrane permeability. CDs with a slightly positive charge interacted with the surface of the negative-charged liposomes and evidence indicated that the association of CDs with the membrane affects the structural and thermodynamic properties of the bilayer; most importantly, it enhances the bilayer’s permeability against doxorubicin, a well-known anticancer drug. The results, like those of similar studies that surveyed the interaction of proteins with lipid membranes, suggest that carbon dots are partially embedded in the bilayer. In vitro experiments employing breast cancer cell lines and human healthy dermal cells corroborated the findings, as it was shown that the presence of CDs in the culture medium selectively enhanced cell internalization of doxorubicin and, subsequently, increased its cytotoxicity, acting as a drug sensitizer. MDPI 2023-06-02 /pmc/articles/PMC10305404/ /pubmed/37375780 http://dx.doi.org/10.3390/ph16060833 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mavroidi, Barbara
Kaminari, Archontia
Sakellis, Elias
Sideratou, Zili
Tsiourvas, Dimitris
Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery
title Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery
title_full Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery
title_fullStr Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery
title_full_unstemmed Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery
title_short Carbon Dots–Biomembrane Interactions and Their Implications for Cellular Drug Delivery
title_sort carbon dots–biomembrane interactions and their implications for cellular drug delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305404/
https://www.ncbi.nlm.nih.gov/pubmed/37375780
http://dx.doi.org/10.3390/ph16060833
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