Cargando…
Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury
Abnormal levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in human serum are the most sensitive indicator of hepatocellular damage. Because liver-related health problems are directly linked to elevated levels of ALT and AST, it is important to develop accurate and rapid...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305476/ https://www.ncbi.nlm.nih.gov/pubmed/37374056 http://dx.doi.org/10.3390/life13061273 |
_version_ | 1785065742956232704 |
---|---|
author | Chinnappan, Raja Mir, Tanveer Ahmad Alsalameh, Sulaiman Makhzoum, Tariq Adeeb, Salma Al-Kattan, Khaled Yaqinuddin, Ahmed |
author_facet | Chinnappan, Raja Mir, Tanveer Ahmad Alsalameh, Sulaiman Makhzoum, Tariq Adeeb, Salma Al-Kattan, Khaled Yaqinuddin, Ahmed |
author_sort | Chinnappan, Raja |
collection | PubMed |
description | Abnormal levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in human serum are the most sensitive indicator of hepatocellular damage. Because liver-related health problems are directly linked to elevated levels of ALT and AST, it is important to develop accurate and rapid methods to detect these enzymes for the early diagnosis of liver disease and prevention of long-term liver damage. Several analytical methods have been developed for the detection of ALT and AST. However, these methods are based on complex mechanisms and require bulky instruments and laboratories, making them unsuitable for point-of-care application or in-house testing. Lateral flow assay (LFA)-based biosensors, on the other hand, provide rapid, accurate, and reliable results, are easy to operate, and are affordable for low-income populations. However, due to the storage, stability, batch-to-batch variations, and error margins, antibody-based LFAs are considered unaffordable for field applications. In this hypothesis, we propose the selection of aptamers with high affinity and specificity for the liver biomarkers ALT and AST to build an efficient LFA device for point-of-care applications. Though the aptamer-based LFA would be semiquantitative for ALT and AST, it would be an inexpensive option for the early detection and diagnosis of liver disease. Aptamer-based LFA is anticipated to minimize the economic burden. It can also be used for routine liver function tests regardless of the economic situation in each country. By developing a low-cost testing platform, millions of patients suffering from liver disease can be saved. |
format | Online Article Text |
id | pubmed-10305476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103054762023-06-29 Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury Chinnappan, Raja Mir, Tanveer Ahmad Alsalameh, Sulaiman Makhzoum, Tariq Adeeb, Salma Al-Kattan, Khaled Yaqinuddin, Ahmed Life (Basel) Hypothesis Abnormal levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in human serum are the most sensitive indicator of hepatocellular damage. Because liver-related health problems are directly linked to elevated levels of ALT and AST, it is important to develop accurate and rapid methods to detect these enzymes for the early diagnosis of liver disease and prevention of long-term liver damage. Several analytical methods have been developed for the detection of ALT and AST. However, these methods are based on complex mechanisms and require bulky instruments and laboratories, making them unsuitable for point-of-care application or in-house testing. Lateral flow assay (LFA)-based biosensors, on the other hand, provide rapid, accurate, and reliable results, are easy to operate, and are affordable for low-income populations. However, due to the storage, stability, batch-to-batch variations, and error margins, antibody-based LFAs are considered unaffordable for field applications. In this hypothesis, we propose the selection of aptamers with high affinity and specificity for the liver biomarkers ALT and AST to build an efficient LFA device for point-of-care applications. Though the aptamer-based LFA would be semiquantitative for ALT and AST, it would be an inexpensive option for the early detection and diagnosis of liver disease. Aptamer-based LFA is anticipated to minimize the economic burden. It can also be used for routine liver function tests regardless of the economic situation in each country. By developing a low-cost testing platform, millions of patients suffering from liver disease can be saved. MDPI 2023-05-29 /pmc/articles/PMC10305476/ /pubmed/37374056 http://dx.doi.org/10.3390/life13061273 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Hypothesis Chinnappan, Raja Mir, Tanveer Ahmad Alsalameh, Sulaiman Makhzoum, Tariq Adeeb, Salma Al-Kattan, Khaled Yaqinuddin, Ahmed Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury |
title | Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury |
title_full | Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury |
title_fullStr | Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury |
title_full_unstemmed | Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury |
title_short | Aptasensors Are Conjectured as Promising ALT and AST Diagnostic Tools for the Early Diagnosis of Acute Liver Injury |
title_sort | aptasensors are conjectured as promising alt and ast diagnostic tools for the early diagnosis of acute liver injury |
topic | Hypothesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305476/ https://www.ncbi.nlm.nih.gov/pubmed/37374056 http://dx.doi.org/10.3390/life13061273 |
work_keys_str_mv | AT chinnappanraja aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury AT mirtanveerahmad aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury AT alsalamehsulaiman aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury AT makhzoumtariq aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury AT adeebsalma aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury AT alkattankhaled aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury AT yaqinuddinahmed aptasensorsareconjecturedaspromisingaltandastdiagnostictoolsfortheearlydiagnosisofacuteliverinjury |