Cargando…

Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage

Stripe rust, which is caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid Thinopyrum elongatum (2n = 4x = 28, EEE...

Descripción completa

Detalles Bibliográficos
Autores principales: Gong, Biran, Zhao, Lei, Zeng, Chunyan, Zhu, Wei, Xu, Lili, Wu, Dandan, Cheng, Yiran, Wang, Yi, Zeng, Jian, Fan, Xing, Sha, Lina, Zhang, Haiqin, Chen, Guoyue, Zhou, Yonghong, Kang, Houyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305598/
https://www.ncbi.nlm.nih.gov/pubmed/37375936
http://dx.doi.org/10.3390/plants12122311
_version_ 1785065772236668928
author Gong, Biran
Zhao, Lei
Zeng, Chunyan
Zhu, Wei
Xu, Lili
Wu, Dandan
Cheng, Yiran
Wang, Yi
Zeng, Jian
Fan, Xing
Sha, Lina
Zhang, Haiqin
Chen, Guoyue
Zhou, Yonghong
Kang, Houyang
author_facet Gong, Biran
Zhao, Lei
Zeng, Chunyan
Zhu, Wei
Xu, Lili
Wu, Dandan
Cheng, Yiran
Wang, Yi
Zeng, Jian
Fan, Xing
Sha, Lina
Zhang, Haiqin
Chen, Guoyue
Zhou, Yonghong
Kang, Houyang
author_sort Gong, Biran
collection PubMed
description Stripe rust, which is caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid Thinopyrum elongatum (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid Th. elongatum 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid Th. elongatum, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid Th. elongatum, which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish Th. elongatum from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid Th. elongatum.
format Online
Article
Text
id pubmed-10305598
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103055982023-06-29 Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage Gong, Biran Zhao, Lei Zeng, Chunyan Zhu, Wei Xu, Lili Wu, Dandan Cheng, Yiran Wang, Yi Zeng, Jian Fan, Xing Sha, Lina Zhang, Haiqin Chen, Guoyue Zhou, Yonghong Kang, Houyang Plants (Basel) Article Stripe rust, which is caused by Puccinia striiformis f. sp. tritici, is one of the most devastating foliar diseases of common wheat worldwide. Breeding new wheat varieties with durable resistance is the most effective way of controlling the disease. Tetraploid Thinopyrum elongatum (2n = 4x = 28, EEEE) carries a variety of genes conferring resistance to multiple diseases, including stripe rust, Fusarium head blight, and powdery mildew, which makes it a valuable tertiary genetic resource for enhancing wheat cultivar improvement. Here, a novel wheat–tetraploid Th. elongatum 6E (6D) disomic substitution line (K17-1065-4) was characterized using genomic in situ hybridization and fluorescence in situ hybridization chromosome painting analyses. The evaluation of disease responses revealed that K17-1065-4 is highly resistant to stripe rust at the adult stage. By analyzing the whole-genome sequence of diploid Th. elongatum, we detected 3382 specific SSR sequences on chromosome 6E. Sixty SSR markers were developed, and thirty-three of them can accurately trace chromosome 6E of tetraploid Th. elongatum, which were linked to the disease resistance gene(s) in the wheat genetic background. The molecular marker analysis indicated that 10 markers may be used to distinguish Th. elongatum from other wheat-related species. Thus, K17-1065-4 carrying the stripe rust resistance gene(s) is a novel germplasm useful for breeding disease-resistant wheat cultivars. The molecular markers developed in this study may facilitate the mapping of the stripe rust resistance gene on chromosome 6E of tetraploid Th. elongatum. MDPI 2023-06-14 /pmc/articles/PMC10305598/ /pubmed/37375936 http://dx.doi.org/10.3390/plants12122311 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gong, Biran
Zhao, Lei
Zeng, Chunyan
Zhu, Wei
Xu, Lili
Wu, Dandan
Cheng, Yiran
Wang, Yi
Zeng, Jian
Fan, Xing
Sha, Lina
Zhang, Haiqin
Chen, Guoyue
Zhou, Yonghong
Kang, Houyang
Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
title Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
title_full Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
title_fullStr Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
title_full_unstemmed Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
title_short Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage
title_sort development and characterization of a novel wheat–tetraploid thinopyrum elongatum 6e (6d) disomic substitution line with stripe rust resistance at the adult stage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305598/
https://www.ncbi.nlm.nih.gov/pubmed/37375936
http://dx.doi.org/10.3390/plants12122311
work_keys_str_mv AT gongbiran developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT zhaolei developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT zengchunyan developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT zhuwei developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT xulili developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT wudandan developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT chengyiran developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT wangyi developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT zengjian developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT fanxing developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT shalina developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT zhanghaiqin developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT chenguoyue developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT zhouyonghong developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage
AT kanghouyang developmentandcharacterizationofanovelwheattetraploidthinopyrumelongatum6e6ddisomicsubstitutionlinewithstriperustresistanceattheadultstage