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Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples

Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient optio...

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Autores principales: Rial-Berriel, Cristian, Ramos-Luzardo, Álvaro, Acosta-Dacal, Andrea, Macías-Montes, Ana, Fernández-Valerón, Pilar, Henríquez-Hernández, Luis Alberto, Zumbado, Manuel, Boada, Luis D., Luzardo, Octavio P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305645/
https://www.ncbi.nlm.nih.gov/pubmed/37368598
http://dx.doi.org/10.3390/toxics11060498
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author Rial-Berriel, Cristian
Ramos-Luzardo, Álvaro
Acosta-Dacal, Andrea
Macías-Montes, Ana
Fernández-Valerón, Pilar
Henríquez-Hernández, Luis Alberto
Zumbado, Manuel
Boada, Luis D.
Luzardo, Octavio P.
author_facet Rial-Berriel, Cristian
Ramos-Luzardo, Álvaro
Acosta-Dacal, Andrea
Macías-Montes, Ana
Fernández-Valerón, Pilar
Henríquez-Hernández, Luis Alberto
Zumbado, Manuel
Boada, Luis D.
Luzardo, Octavio P.
author_sort Rial-Berriel, Cristian
collection PubMed
description Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient options. In this study, we have applied and validated a method for detecting several hundred toxins in serum samples. This technique involved a single-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by analysis using gas and liquid chromatography coupled with mass spectrometry. With this methodology, we could detect and quantify up to 353 compounds, including persistent organic pollutants (POPs), pesticides, pharmaceuticals, and rodenticides, using just 250 µL of serum. Among them, 92% could be measured at concentrations below 1.25 ng/mL, making it ideal for biomonitoring. We applied this method to samples collected from camels (n = 40) and humans (n = 25). We detected naproxen, ketoprofen, paracetamol, levamisole, and some POPs in these samples. This study validated the ability to simultaneously detect a broad range of compounds in small volumes of serum.
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spelling pubmed-103056452023-06-29 Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples Rial-Berriel, Cristian Ramos-Luzardo, Álvaro Acosta-Dacal, Andrea Macías-Montes, Ana Fernández-Valerón, Pilar Henríquez-Hernández, Luis Alberto Zumbado, Manuel Boada, Luis D. Luzardo, Octavio P. Toxics Article Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient options. In this study, we have applied and validated a method for detecting several hundred toxins in serum samples. This technique involved a single-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by analysis using gas and liquid chromatography coupled with mass spectrometry. With this methodology, we could detect and quantify up to 353 compounds, including persistent organic pollutants (POPs), pesticides, pharmaceuticals, and rodenticides, using just 250 µL of serum. Among them, 92% could be measured at concentrations below 1.25 ng/mL, making it ideal for biomonitoring. We applied this method to samples collected from camels (n = 40) and humans (n = 25). We detected naproxen, ketoprofen, paracetamol, levamisole, and some POPs in these samples. This study validated the ability to simultaneously detect a broad range of compounds in small volumes of serum. MDPI 2023-05-31 /pmc/articles/PMC10305645/ /pubmed/37368598 http://dx.doi.org/10.3390/toxics11060498 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rial-Berriel, Cristian
Ramos-Luzardo, Álvaro
Acosta-Dacal, Andrea
Macías-Montes, Ana
Fernández-Valerón, Pilar
Henríquez-Hernández, Luis Alberto
Zumbado, Manuel
Boada, Luis D.
Luzardo, Octavio P.
Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
title Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
title_full Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
title_fullStr Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
title_full_unstemmed Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
title_short Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
title_sort validation of a method scope extension for simple biomonitoring of 353 pollutants in serum samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305645/
https://www.ncbi.nlm.nih.gov/pubmed/37368598
http://dx.doi.org/10.3390/toxics11060498
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