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Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection
We propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor. Biomolecul...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305706/ https://www.ncbi.nlm.nih.gov/pubmed/37376252 http://dx.doi.org/10.3390/polym15122607 |
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author | Shakurov, Ruslan Sizova, Svetlana Dudik, Stepan Serkina, Anna Bazhutov, Mark Stanaityte, Viktorija Tulyagin, Petr Konopsky, Valery Alieva, Elena Sekatskii, Sergey Bespyatykh, Julia Basmanov, Dmitry |
author_facet | Shakurov, Ruslan Sizova, Svetlana Dudik, Stepan Serkina, Anna Bazhutov, Mark Stanaityte, Viktorija Tulyagin, Petr Konopsky, Valery Alieva, Elena Sekatskii, Sergey Bespyatykh, Julia Basmanov, Dmitry |
author_sort | Shakurov, Ruslan |
collection | PubMed |
description | We propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor. Biomolecule sorption is detected by measuring changes in the parameters of optical modes on the surface of a photonic crystal (PC). We describe the step-by-step biochip fabrication process. Using oligonucleotides as small molecules and PC SM visualization in a microfluidic mode, we show that the PAMAM (poly-amidoamine)-modified chip’s sorption efficiency is almost 14 times higher than that of the planar aminosilane layer and 5 times higher than the 3D epoxy-dextran matrix. The results obtained demonstrate a promising direction for further development of the dendrimer-based PC SM sensor method as an advanced label-free microfluidic tool for detecting biomolecule interactions. Current label-free methods for small biomolecule detection, such as surface plasmon resonance (SPR), have a detection limit down to pM. In this work, we achieved for a PC SM biosensor a Limit of Quantitation of up to 70 fM, which is comparable with the best label-using methods without their inherent disadvantages, such as changes in molecular activity caused by labeling. |
format | Online Article Text |
id | pubmed-10305706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103057062023-06-29 Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection Shakurov, Ruslan Sizova, Svetlana Dudik, Stepan Serkina, Anna Bazhutov, Mark Stanaityte, Viktorija Tulyagin, Petr Konopsky, Valery Alieva, Elena Sekatskii, Sergey Bespyatykh, Julia Basmanov, Dmitry Polymers (Basel) Article We propose and demonstrate dendrimer-based coatings for a sensitive biochip surface that enhance the high-performance sorption of small molecules (i.e., biomolecules with low molecular weights) and the sensitivity of a label-free, real-time photonic crystal surface mode (PC SM) biosensor. Biomolecule sorption is detected by measuring changes in the parameters of optical modes on the surface of a photonic crystal (PC). We describe the step-by-step biochip fabrication process. Using oligonucleotides as small molecules and PC SM visualization in a microfluidic mode, we show that the PAMAM (poly-amidoamine)-modified chip’s sorption efficiency is almost 14 times higher than that of the planar aminosilane layer and 5 times higher than the 3D epoxy-dextran matrix. The results obtained demonstrate a promising direction for further development of the dendrimer-based PC SM sensor method as an advanced label-free microfluidic tool for detecting biomolecule interactions. Current label-free methods for small biomolecule detection, such as surface plasmon resonance (SPR), have a detection limit down to pM. In this work, we achieved for a PC SM biosensor a Limit of Quantitation of up to 70 fM, which is comparable with the best label-using methods without their inherent disadvantages, such as changes in molecular activity caused by labeling. MDPI 2023-06-08 /pmc/articles/PMC10305706/ /pubmed/37376252 http://dx.doi.org/10.3390/polym15122607 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shakurov, Ruslan Sizova, Svetlana Dudik, Stepan Serkina, Anna Bazhutov, Mark Stanaityte, Viktorija Tulyagin, Petr Konopsky, Valery Alieva, Elena Sekatskii, Sergey Bespyatykh, Julia Basmanov, Dmitry Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection |
title | Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection |
title_full | Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection |
title_fullStr | Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection |
title_full_unstemmed | Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection |
title_short | Dendrimer-Based Coatings on a Photonic Crystal Surface for Ultra-Sensitive Small Molecule Detection |
title_sort | dendrimer-based coatings on a photonic crystal surface for ultra-sensitive small molecule detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305706/ https://www.ncbi.nlm.nih.gov/pubmed/37376252 http://dx.doi.org/10.3390/polym15122607 |
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