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Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase

Antimicrobial resistance has become a growing public health concern in recent decades, demanding a search for new effective treatments. Therefore, this study aimed to elucidate the phytochemical composition and evaluate the antibacterial activity of the essential oil obtained from the fruits of Pipe...

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Autores principales: da Silva, Lucas Yure Santos, Paulo, Cicera Laura Roque, Moura, Talysson Felismino, Alves, Daniel Sampaio, Pessoa, Renata Torres, Araújo, Isaac Moura, de Morais Oliveira-Tintino, Cícera Datiane, Tintino, Saulo Relison, Nonato, Carla de Fatima Alves, da Costa, José Galberto Martins, Ribeiro-Filho, Jaime, Coutinho, Henrique Douglas Melo, Kowalska, Grażyna, Mitura, Przemysław, Bar, Marek, Kowalski, Radosław, de Menezes, Irwin Rose Alencar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305721/
https://www.ncbi.nlm.nih.gov/pubmed/37376002
http://dx.doi.org/10.3390/plants12122377
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author da Silva, Lucas Yure Santos
Paulo, Cicera Laura Roque
Moura, Talysson Felismino
Alves, Daniel Sampaio
Pessoa, Renata Torres
Araújo, Isaac Moura
de Morais Oliveira-Tintino, Cícera Datiane
Tintino, Saulo Relison
Nonato, Carla de Fatima Alves
da Costa, José Galberto Martins
Ribeiro-Filho, Jaime
Coutinho, Henrique Douglas Melo
Kowalska, Grażyna
Mitura, Przemysław
Bar, Marek
Kowalski, Radosław
de Menezes, Irwin Rose Alencar
author_facet da Silva, Lucas Yure Santos
Paulo, Cicera Laura Roque
Moura, Talysson Felismino
Alves, Daniel Sampaio
Pessoa, Renata Torres
Araújo, Isaac Moura
de Morais Oliveira-Tintino, Cícera Datiane
Tintino, Saulo Relison
Nonato, Carla de Fatima Alves
da Costa, José Galberto Martins
Ribeiro-Filho, Jaime
Coutinho, Henrique Douglas Melo
Kowalska, Grażyna
Mitura, Przemysław
Bar, Marek
Kowalski, Radosław
de Menezes, Irwin Rose Alencar
author_sort da Silva, Lucas Yure Santos
collection PubMed
description Antimicrobial resistance has become a growing public health concern in recent decades, demanding a search for new effective treatments. Therefore, this study aimed to elucidate the phytochemical composition and evaluate the antibacterial activity of the essential oil obtained from the fruits of Piper tuberculatum Jacq. (EOPT) against strains carrying different mechanisms of antibiotic resistance. Phytochemical analysis was performed using gas chromatography–mass spectrometry (GC/MS). The antibacterial activity of EOPT and its ability to inhibit antibiotic resistance was evaluated through the broth microdilution method. The GC-MS analysis identified 99.59% of the constituents, with β-pinene (31.51%), α-pinene (28.38%), and β-cis-ocimene (20.22%) being identified as major constituents. The minimum inhibitory concentration (MIC) of EOPT was determined to assess its antibacterial activity against multidrug-resistant strains of Staphylococcus aureus (IS-58, 1199B, K2068, and K4100). The compound showed a MIC of ≥ 1024 μg/mL, suggesting a lack of intrinsic antibacterial activity. However, when the EOPT was associated with antibiotics and EtBr, a significant decrease in antibiotic resistance was observed, indicating the modulation of efflux pump activity. This evidence was corroborated with the observation of increased fluorescent light emission by the bacterial strains, indicating the involvement of the NorA and MepA efflux pumps. Additionally, the significant potentiation of ampicillin activity against the S. aureus strain K4414 suggests the β-lactamase inhibitory activity of EOPT. These results suggest that the essential oil from P. tuberculatum fruits has antibiotic-enhancing properties, with a mechanism involving the inhibition of efflux pumps and β-lactamase in MDR S. aureus strains. These findings provide new perspectives on the potential use of EOPT against antibiotic resistance and highlight the importance of Piper species as sources of bioactive compounds with promising therapeutic activities against MDR bacteria. Nevertheless, further preclinical (in vivo) studies remain necessary to confirm these in vitro-observed results.
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spelling pubmed-103057212023-06-29 Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase da Silva, Lucas Yure Santos Paulo, Cicera Laura Roque Moura, Talysson Felismino Alves, Daniel Sampaio Pessoa, Renata Torres Araújo, Isaac Moura de Morais Oliveira-Tintino, Cícera Datiane Tintino, Saulo Relison Nonato, Carla de Fatima Alves da Costa, José Galberto Martins Ribeiro-Filho, Jaime Coutinho, Henrique Douglas Melo Kowalska, Grażyna Mitura, Przemysław Bar, Marek Kowalski, Radosław de Menezes, Irwin Rose Alencar Plants (Basel) Article Antimicrobial resistance has become a growing public health concern in recent decades, demanding a search for new effective treatments. Therefore, this study aimed to elucidate the phytochemical composition and evaluate the antibacterial activity of the essential oil obtained from the fruits of Piper tuberculatum Jacq. (EOPT) against strains carrying different mechanisms of antibiotic resistance. Phytochemical analysis was performed using gas chromatography–mass spectrometry (GC/MS). The antibacterial activity of EOPT and its ability to inhibit antibiotic resistance was evaluated through the broth microdilution method. The GC-MS analysis identified 99.59% of the constituents, with β-pinene (31.51%), α-pinene (28.38%), and β-cis-ocimene (20.22%) being identified as major constituents. The minimum inhibitory concentration (MIC) of EOPT was determined to assess its antibacterial activity against multidrug-resistant strains of Staphylococcus aureus (IS-58, 1199B, K2068, and K4100). The compound showed a MIC of ≥ 1024 μg/mL, suggesting a lack of intrinsic antibacterial activity. However, when the EOPT was associated with antibiotics and EtBr, a significant decrease in antibiotic resistance was observed, indicating the modulation of efflux pump activity. This evidence was corroborated with the observation of increased fluorescent light emission by the bacterial strains, indicating the involvement of the NorA and MepA efflux pumps. Additionally, the significant potentiation of ampicillin activity against the S. aureus strain K4414 suggests the β-lactamase inhibitory activity of EOPT. These results suggest that the essential oil from P. tuberculatum fruits has antibiotic-enhancing properties, with a mechanism involving the inhibition of efflux pumps and β-lactamase in MDR S. aureus strains. These findings provide new perspectives on the potential use of EOPT against antibiotic resistance and highlight the importance of Piper species as sources of bioactive compounds with promising therapeutic activities against MDR bacteria. Nevertheless, further preclinical (in vivo) studies remain necessary to confirm these in vitro-observed results. MDPI 2023-06-19 /pmc/articles/PMC10305721/ /pubmed/37376002 http://dx.doi.org/10.3390/plants12122377 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Lucas Yure Santos
Paulo, Cicera Laura Roque
Moura, Talysson Felismino
Alves, Daniel Sampaio
Pessoa, Renata Torres
Araújo, Isaac Moura
de Morais Oliveira-Tintino, Cícera Datiane
Tintino, Saulo Relison
Nonato, Carla de Fatima Alves
da Costa, José Galberto Martins
Ribeiro-Filho, Jaime
Coutinho, Henrique Douglas Melo
Kowalska, Grażyna
Mitura, Przemysław
Bar, Marek
Kowalski, Radosław
de Menezes, Irwin Rose Alencar
Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase
title Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase
title_full Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase
title_fullStr Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase
title_full_unstemmed Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase
title_short Antibacterial Activity of the Essential Oil of Piper tuberculatum Jacq. Fruits against Multidrug-Resistant Strains: Inhibition of Efflux Pumps and β-Lactamase
title_sort antibacterial activity of the essential oil of piper tuberculatum jacq. fruits against multidrug-resistant strains: inhibition of efflux pumps and β-lactamase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305721/
https://www.ncbi.nlm.nih.gov/pubmed/37376002
http://dx.doi.org/10.3390/plants12122377
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