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Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal
Wastewater-based epidemiology/wastewater-based surveillance (WBE/WBS) continues to serve as an effective means of monitoring various diseases, including COVID-19 and the emergence of SARS-CoV-2 variants, at the population level. As the use of WBE expands, storage conditions of wastewater samples wil...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307669/ https://www.ncbi.nlm.nih.gov/pubmed/37392884 http://dx.doi.org/10.1016/j.scitotenv.2023.165098 |
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author | Thapar, Isha Langan, Laura M. Davis, Haley Norman, R. Sean Bojes, Heidi K. Brooks, Bryan W. |
author_facet | Thapar, Isha Langan, Laura M. Davis, Haley Norman, R. Sean Bojes, Heidi K. Brooks, Bryan W. |
author_sort | Thapar, Isha |
collection | PubMed |
description | Wastewater-based epidemiology/wastewater-based surveillance (WBE/WBS) continues to serve as an effective means of monitoring various diseases, including COVID-19 and the emergence of SARS-CoV-2 variants, at the population level. As the use of WBE expands, storage conditions of wastewater samples will play a critical role in ensuring the accuracy and reproducibility of the generated results. In this study, the impacts of water concentration buffer (WCB), storage temperature, and freeze-thaw cycles on the detection of SARS-CoV-2 and other WBE-related gene targets were quantified. Freeze-thawing of concentrated samples did not have a significant effect on crossing/cycle threshold (Ct) value for any of the gene targets tested (SARS-CoV-2 N1, PMMoV, and BCoV). However, use of WCB during concentration resulted in a significant (p < 0.05) decrease in Ct for all targets and storage at −80 °C (in contrast to −20 °C) appeared preferable for wastewater storage signal stability based on decreased Ct values, although this was only significantly different (p < 0.05) for the BCoV target. Interestingly, when Ct values were converted to gene copies per influent sample, no significant differences (p > 0.05) were observed in any of the targets examined. The stability of RNA targets in concentrated wastewater against freeze-thaw degradation supports the archiving of concentrated samples for use in retrospective examination of COVID-19 trends and tracing SARS-CoV-2 variants, in addition to other potential viruses, and provides a starting point for establishing a consistent procedure for specimen collection and storage for the WBE/WBS community. |
format | Online Article Text |
id | pubmed-10307669 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103076692023-06-29 Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal Thapar, Isha Langan, Laura M. Davis, Haley Norman, R. Sean Bojes, Heidi K. Brooks, Bryan W. Sci Total Environ Article Wastewater-based epidemiology/wastewater-based surveillance (WBE/WBS) continues to serve as an effective means of monitoring various diseases, including COVID-19 and the emergence of SARS-CoV-2 variants, at the population level. As the use of WBE expands, storage conditions of wastewater samples will play a critical role in ensuring the accuracy and reproducibility of the generated results. In this study, the impacts of water concentration buffer (WCB), storage temperature, and freeze-thaw cycles on the detection of SARS-CoV-2 and other WBE-related gene targets were quantified. Freeze-thawing of concentrated samples did not have a significant effect on crossing/cycle threshold (Ct) value for any of the gene targets tested (SARS-CoV-2 N1, PMMoV, and BCoV). However, use of WCB during concentration resulted in a significant (p < 0.05) decrease in Ct for all targets and storage at −80 °C (in contrast to −20 °C) appeared preferable for wastewater storage signal stability based on decreased Ct values, although this was only significantly different (p < 0.05) for the BCoV target. Interestingly, when Ct values were converted to gene copies per influent sample, no significant differences (p > 0.05) were observed in any of the targets examined. The stability of RNA targets in concentrated wastewater against freeze-thaw degradation supports the archiving of concentrated samples for use in retrospective examination of COVID-19 trends and tracing SARS-CoV-2 variants, in addition to other potential viruses, and provides a starting point for establishing a consistent procedure for specimen collection and storage for the WBE/WBS community. Published by Elsevier B.V. 2023-06-29 /pmc/articles/PMC10307669/ /pubmed/37392884 http://dx.doi.org/10.1016/j.scitotenv.2023.165098 Text en © 2023 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Thapar, Isha Langan, Laura M. Davis, Haley Norman, R. Sean Bojes, Heidi K. Brooks, Bryan W. Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal |
title | Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal |
title_full | Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal |
title_fullStr | Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal |
title_full_unstemmed | Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal |
title_short | Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal |
title_sort | influence of storage conditions and multiple freeze-thaw cycles on n1 sars-cov-2, pmmov, and bcov signal |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307669/ https://www.ncbi.nlm.nih.gov/pubmed/37392884 http://dx.doi.org/10.1016/j.scitotenv.2023.165098 |
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