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Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure
INTRODUCTION: Qishen Granule (QSG), a clinically approved traditional Chinese medicine, has been researched for treating heart failure (HF) for many years. However, the effect of QSG on intestinal microecology remains unconfirmed. Therefore, this study aimed to elucidate the possible mechanism of QS...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307979/ https://www.ncbi.nlm.nih.gov/pubmed/37396388 http://dx.doi.org/10.3389/fmicb.2023.1202768 |
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author | Gao, Kuo Yu, Xue Li, Fanghe Huang, Yiran Liu, Jiali Liu, Siqi Lu, Linghui Yang, Ran Wang, Chao |
author_facet | Gao, Kuo Yu, Xue Li, Fanghe Huang, Yiran Liu, Jiali Liu, Siqi Lu, Linghui Yang, Ran Wang, Chao |
author_sort | Gao, Kuo |
collection | PubMed |
description | INTRODUCTION: Qishen Granule (QSG), a clinically approved traditional Chinese medicine, has been researched for treating heart failure (HF) for many years. However, the effect of QSG on intestinal microecology remains unconfirmed. Therefore, this study aimed to elucidate the possible mechanism of QSG regulating HF in rats based on intestinal microecological changes. METHODS: A rat model with HF induced by myocardial infarction was prepared by left coronary artery ligation. Cardiac functions were assessed by echocardiography, pathological changes in the heart and ileum by hematoxylin–eosin (HE) and Masson staining, mitochondrial ultrastructure by transmission electron microscope, and gut microbiota by 16S rRNA sequencing. RESULTS: QSG administration improved cardiac function, tightened cardiomyocytes alignment, decreased fibrous tissue and collagen deposition, and reduced inflammatory cell infiltration. Electron microscopic observation of mitochondria revealed that QSG could arrange mitochondria neatly, reduce swelling, and improve the structural integrity of the crest. Firmicutes were the dominant component in the model group, and QSG could significantly increase the abundance of Bacteroidetes and Prevotellaceae_NK3B31_group. Furthermore, QSG significantly reduced plasma lipopolysaccharide (LPS), improved intestinal structure, and recovered barrier protection function in rats with HF. CONCLUSION: These results demonstrated that QSG was able to improve cardiac function by regulating intestinal microecology in rats with HF, suggesting promising therapeutic targets for HF. |
format | Online Article Text |
id | pubmed-10307979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103079792023-06-30 Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure Gao, Kuo Yu, Xue Li, Fanghe Huang, Yiran Liu, Jiali Liu, Siqi Lu, Linghui Yang, Ran Wang, Chao Front Microbiol Microbiology INTRODUCTION: Qishen Granule (QSG), a clinically approved traditional Chinese medicine, has been researched for treating heart failure (HF) for many years. However, the effect of QSG on intestinal microecology remains unconfirmed. Therefore, this study aimed to elucidate the possible mechanism of QSG regulating HF in rats based on intestinal microecological changes. METHODS: A rat model with HF induced by myocardial infarction was prepared by left coronary artery ligation. Cardiac functions were assessed by echocardiography, pathological changes in the heart and ileum by hematoxylin–eosin (HE) and Masson staining, mitochondrial ultrastructure by transmission electron microscope, and gut microbiota by 16S rRNA sequencing. RESULTS: QSG administration improved cardiac function, tightened cardiomyocytes alignment, decreased fibrous tissue and collagen deposition, and reduced inflammatory cell infiltration. Electron microscopic observation of mitochondria revealed that QSG could arrange mitochondria neatly, reduce swelling, and improve the structural integrity of the crest. Firmicutes were the dominant component in the model group, and QSG could significantly increase the abundance of Bacteroidetes and Prevotellaceae_NK3B31_group. Furthermore, QSG significantly reduced plasma lipopolysaccharide (LPS), improved intestinal structure, and recovered barrier protection function in rats with HF. CONCLUSION: These results demonstrated that QSG was able to improve cardiac function by regulating intestinal microecology in rats with HF, suggesting promising therapeutic targets for HF. Frontiers Media S.A. 2023-06-15 /pmc/articles/PMC10307979/ /pubmed/37396388 http://dx.doi.org/10.3389/fmicb.2023.1202768 Text en Copyright © 2023 Gao, Yu, Li, Huang, Liu, Liu, Lu, Yang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gao, Kuo Yu, Xue Li, Fanghe Huang, Yiran Liu, Jiali Liu, Siqi Lu, Linghui Yang, Ran Wang, Chao Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
title | Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
title_full | Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
title_fullStr | Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
title_full_unstemmed | Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
title_short | Qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
title_sort | qishen granules regulate intestinal microecology to improve cardiac function in rats with heart failure |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307979/ https://www.ncbi.nlm.nih.gov/pubmed/37396388 http://dx.doi.org/10.3389/fmicb.2023.1202768 |
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