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Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons

A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to th...

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Autores principales: Mangalath, Aswathy, Raj, Vishnu, Santhosh, Rasitha, Thekkuveettil, Anoopkumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308187/
https://www.ncbi.nlm.nih.gov/pubmed/37397799
http://dx.doi.org/10.21769/BioProtoc.4697
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author Mangalath, Aswathy
Raj, Vishnu
Santhosh, Rasitha
Thekkuveettil, Anoopkumar
author_facet Mangalath, Aswathy
Raj, Vishnu
Santhosh, Rasitha
Thekkuveettil, Anoopkumar
author_sort Mangalath, Aswathy
collection PubMed
description A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation.
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spelling pubmed-103081872023-06-30 Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons Mangalath, Aswathy Raj, Vishnu Santhosh, Rasitha Thekkuveettil, Anoopkumar Bio Protoc Methods Article A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation. Bio-Protocol 2023-06-20 /pmc/articles/PMC10308187/ /pubmed/37397799 http://dx.doi.org/10.21769/BioProtoc.4697 Text en ©Copyright : © 2023 The Authors; This is an open access article under the CC BY-NC license https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Methods Article
Mangalath, Aswathy
Raj, Vishnu
Santhosh, Rasitha
Thekkuveettil, Anoopkumar
Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
title Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
title_full Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
title_fullStr Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
title_full_unstemmed Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
title_short Alginate Gel Immobilization of Caenorhabditis elegans for Optical Calcium Imaging of Neurons
title_sort alginate gel immobilization of caenorhabditis elegans for optical calcium imaging of neurons
topic Methods Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308187/
https://www.ncbi.nlm.nih.gov/pubmed/37397799
http://dx.doi.org/10.21769/BioProtoc.4697
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