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User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection
To date, a range of nanozymes has been reported for their enzyme-mimicking catalytic activity such as solution-based sensors. However, in remote areas, the need for portable, cost-effective, and one-pot prepared sensors is obvious. In this study, we report the development of a highly stable and sens...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308203/ https://www.ncbi.nlm.nih.gov/pubmed/37397283 http://dx.doi.org/10.1039/d3ra03073c |
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author | Al-Kassawneh, Muna Sadiq, Zubi Jahanshahi-Anbuhi, Sana |
author_facet | Al-Kassawneh, Muna Sadiq, Zubi Jahanshahi-Anbuhi, Sana |
author_sort | Al-Kassawneh, Muna |
collection | PubMed |
description | To date, a range of nanozymes has been reported for their enzyme-mimicking catalytic activity such as solution-based sensors. However, in remote areas, the need for portable, cost-effective, and one-pot prepared sensors is obvious. In this study, we report the development of a highly stable and sensitive gold tablet-based sensor for cysteamine quantification in human serum samples. The sensor is produced in two steps: synthesis of a pullulan-stabilized gold nanoparticle solution (pAuNP-Solution) using a pullulan polymer as a reducing, stabilizing, and encapsulating agent and then, casting the pAuNP-Solution into a pullulan gold nanoparticle tablet (pAuNP-Tablet) by a pipetting method. The tablet was characterized by UV-vis, DLS, FTIR, TEM, and AFM analyses. The pAuNP-tablet exhibited a high peroxidase-mimetic activity via a TMB–H(2)O(2) system. The presence of cysteamine in the system introduced two types of inhibition which were dependent on the cysteamine concentration. By determining Michaelis–Menten's kinetic parameters, we gained mechanistic insights into the catalytic inhibition process. Based on the catalytic inhibition capability of cysteamine, the limit of detection (LoD) was calculated to be 69.04 and 82.9 μM in buffer and human serum samples, respectively. Finally, real human serum samples were tested, demonstrating the applicability of the pAuNP-Tablet for real-world applications. The % R values in human serum samples were in the range of 91–105% with % RSD less than 2% for all replicas. The stability tests over 16 months revealed the ultra-stable properties of the pAuNP-Tablet. Overall, with a simple fabrication method and a novel employed technique, this study contributes to the advancement of tablet-based sensors and helps in cysteamine detection in clinical settings. |
format | Online Article Text |
id | pubmed-10308203 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-103082032023-06-30 User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection Al-Kassawneh, Muna Sadiq, Zubi Jahanshahi-Anbuhi, Sana RSC Adv Chemistry To date, a range of nanozymes has been reported for their enzyme-mimicking catalytic activity such as solution-based sensors. However, in remote areas, the need for portable, cost-effective, and one-pot prepared sensors is obvious. In this study, we report the development of a highly stable and sensitive gold tablet-based sensor for cysteamine quantification in human serum samples. The sensor is produced in two steps: synthesis of a pullulan-stabilized gold nanoparticle solution (pAuNP-Solution) using a pullulan polymer as a reducing, stabilizing, and encapsulating agent and then, casting the pAuNP-Solution into a pullulan gold nanoparticle tablet (pAuNP-Tablet) by a pipetting method. The tablet was characterized by UV-vis, DLS, FTIR, TEM, and AFM analyses. The pAuNP-tablet exhibited a high peroxidase-mimetic activity via a TMB–H(2)O(2) system. The presence of cysteamine in the system introduced two types of inhibition which were dependent on the cysteamine concentration. By determining Michaelis–Menten's kinetic parameters, we gained mechanistic insights into the catalytic inhibition process. Based on the catalytic inhibition capability of cysteamine, the limit of detection (LoD) was calculated to be 69.04 and 82.9 μM in buffer and human serum samples, respectively. Finally, real human serum samples were tested, demonstrating the applicability of the pAuNP-Tablet for real-world applications. The % R values in human serum samples were in the range of 91–105% with % RSD less than 2% for all replicas. The stability tests over 16 months revealed the ultra-stable properties of the pAuNP-Tablet. Overall, with a simple fabrication method and a novel employed technique, this study contributes to the advancement of tablet-based sensors and helps in cysteamine detection in clinical settings. The Royal Society of Chemistry 2023-06-29 /pmc/articles/PMC10308203/ /pubmed/37397283 http://dx.doi.org/10.1039/d3ra03073c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Al-Kassawneh, Muna Sadiq, Zubi Jahanshahi-Anbuhi, Sana User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
title | User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
title_full | User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
title_fullStr | User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
title_full_unstemmed | User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
title_short | User-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
title_sort | user-friendly and ultra-stable all-inclusive gold tablets for cysteamine detection |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308203/ https://www.ncbi.nlm.nih.gov/pubmed/37397283 http://dx.doi.org/10.1039/d3ra03073c |
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