Self-cloning of the Catalase Gene in Environmental Isolates Improves Their Colony-forming Abilities on Agar Media

Hydrogen peroxide (H(2)O(2)) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H(2)O(2) concentration in agar plates. Putative catalase genes, which degrade H(2)O(2), were detected in their...

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Detalles Bibliográficos
Autores principales: Watanabe, Motoyuki, Igarashi, Kensuke, Kato, Souichiro, Kamagata, Yoichi, Kitagawa, Wataru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308234/
https://www.ncbi.nlm.nih.gov/pubmed/37302843
http://dx.doi.org/10.1264/jsme2.ME23006
Descripción
Sumario:Hydrogen peroxide (H(2)O(2)) inhibits microbial growth at a specific concentration. However, we previously isolated two environmental bacterial strains that exhibited sensitivity to a lower H(2)O(2) concentration in agar plates. Putative catalase genes, which degrade H(2)O(2), were detected in their genomes. We herein elucidated the characteristics of these putative genes and their products using a self-cloning technique. The products of the cloned genes were identified as functional catalases. The up-regulation of their expression increased the colony-forming ability of host cells under H(2)O(2) pressure. The present results demonstrated high sensitivity to H(2)O(2) even in microbes possessing functional catalase genes.

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