Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva

The C. elegans vulva is a polarized epithelial tube that has been studied extensively as a model for cell-cell signaling, cell fate specification, and tubulogenesis. Here we used endogenous fusions to show that the spectrin cytoskeleton is polarized in this organ, with conventional beta-spectrin ( U...

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Autores principales: Barker, Trevor J., Chan, Fung-Yi, Carvalho, Ana X., Sundaram, Meera V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308243/
https://www.ncbi.nlm.nih.gov/pubmed/37396793
http://dx.doi.org/10.17912/micropub.biology.000863
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author Barker, Trevor J.
Chan, Fung-Yi
Carvalho, Ana X.
Sundaram, Meera V.
author_facet Barker, Trevor J.
Chan, Fung-Yi
Carvalho, Ana X.
Sundaram, Meera V.
author_sort Barker, Trevor J.
collection PubMed
description The C. elegans vulva is a polarized epithelial tube that has been studied extensively as a model for cell-cell signaling, cell fate specification, and tubulogenesis. Here we used endogenous fusions to show that the spectrin cytoskeleton is polarized in this organ, with conventional beta-spectrin ( UNC-70 ) found only at basolateral membranes and beta heavy spectrin ( SMA-1 ) found only at apical membranes. The sole alpha-spectrin ( SPC-1 ) is present at both locations but requires SMA-1 for its apical localization. Thus, beta spectrins are excellent markers for vulva cell membranes and polarity.
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spelling pubmed-103082432023-06-30 Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva Barker, Trevor J. Chan, Fung-Yi Carvalho, Ana X. Sundaram, Meera V. MicroPubl Biol New Finding The C. elegans vulva is a polarized epithelial tube that has been studied extensively as a model for cell-cell signaling, cell fate specification, and tubulogenesis. Here we used endogenous fusions to show that the spectrin cytoskeleton is polarized in this organ, with conventional beta-spectrin ( UNC-70 ) found only at basolateral membranes and beta heavy spectrin ( SMA-1 ) found only at apical membranes. The sole alpha-spectrin ( SPC-1 ) is present at both locations but requires SMA-1 for its apical localization. Thus, beta spectrins are excellent markers for vulva cell membranes and polarity. Caltech Library 2023-06-14 /pmc/articles/PMC10308243/ /pubmed/37396793 http://dx.doi.org/10.17912/micropub.biology.000863 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Barker, Trevor J.
Chan, Fung-Yi
Carvalho, Ana X.
Sundaram, Meera V.
Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva
title Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva
title_full Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva
title_fullStr Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva
title_full_unstemmed Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva
title_short Apical-basal polarity of the spectrin cytoskeleton in the C. elegans vulva
title_sort apical-basal polarity of the spectrin cytoskeleton in the c. elegans vulva
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308243/
https://www.ncbi.nlm.nih.gov/pubmed/37396793
http://dx.doi.org/10.17912/micropub.biology.000863
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