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Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging
[Image: see text] A novel family of julolidine-containing fluorescent rhodols equipped with a wide variety of substituents was synthesized in a versatile two-step process. The prepared compounds were fully characterized and exhibited excellent fluorescence properties for microscopy imaging. The best...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308389/ https://www.ncbi.nlm.nih.gov/pubmed/37396252 http://dx.doi.org/10.1021/acsomega.3c01796 |
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| author | Szepesi Kovács, Dénes Chiovini, Balázs Müller, Dalma Tóth, Estilla Zsófia Fülöp, Anna Ábrányi-Balogh, Péter Wittner, Lucia Várady, György Farkas, Ödön Turczel, Gábor Katona, Gergely Győrffy, Balázs Keserű, György Miklós Mucsi, Zoltán Rózsa, Balázs J. Kovács, Ervin |
| author_facet | Szepesi Kovács, Dénes Chiovini, Balázs Müller, Dalma Tóth, Estilla Zsófia Fülöp, Anna Ábrányi-Balogh, Péter Wittner, Lucia Várady, György Farkas, Ödön Turczel, Gábor Katona, Gergely Győrffy, Balázs Keserű, György Miklós Mucsi, Zoltán Rózsa, Balázs J. Kovács, Ervin |
| author_sort | Szepesi Kovács, Dénes |
| collection | PubMed |
| description | [Image: see text] A novel family of julolidine-containing fluorescent rhodols equipped with a wide variety of substituents was synthesized in a versatile two-step process. The prepared compounds were fully characterized and exhibited excellent fluorescence properties for microscopy imaging. The best candidate was conjugated to the therapeutic antibody trastuzumab through a copper-free strain-promoted azide-alkyne click reaction. The rhodol-labeled antibody was successfully applied for in vitro confocal and two-photon microscopy imaging of Her2+ cells. |
| format | Online Article Text |
| id | pubmed-10308389 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | American Chemical Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-103083892023-06-30 Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging Szepesi Kovács, Dénes Chiovini, Balázs Müller, Dalma Tóth, Estilla Zsófia Fülöp, Anna Ábrányi-Balogh, Péter Wittner, Lucia Várady, György Farkas, Ödön Turczel, Gábor Katona, Gergely Győrffy, Balázs Keserű, György Miklós Mucsi, Zoltán Rózsa, Balázs J. Kovács, Ervin ACS Omega [Image: see text] A novel family of julolidine-containing fluorescent rhodols equipped with a wide variety of substituents was synthesized in a versatile two-step process. The prepared compounds were fully characterized and exhibited excellent fluorescence properties for microscopy imaging. The best candidate was conjugated to the therapeutic antibody trastuzumab through a copper-free strain-promoted azide-alkyne click reaction. The rhodol-labeled antibody was successfully applied for in vitro confocal and two-photon microscopy imaging of Her2+ cells. American Chemical Society 2023-06-14 /pmc/articles/PMC10308389/ /pubmed/37396252 http://dx.doi.org/10.1021/acsomega.3c01796 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
| spellingShingle | Szepesi Kovács, Dénes Chiovini, Balázs Müller, Dalma Tóth, Estilla Zsófia Fülöp, Anna Ábrányi-Balogh, Péter Wittner, Lucia Várady, György Farkas, Ödön Turczel, Gábor Katona, Gergely Győrffy, Balázs Keserű, György Miklós Mucsi, Zoltán Rózsa, Balázs J. Kovács, Ervin Synthesis and Application of Two-Photon Active Fluorescent Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging |
| title | Synthesis and Application
of Two-Photon Active Fluorescent
Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging |
| title_full | Synthesis and Application
of Two-Photon Active Fluorescent
Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging |
| title_fullStr | Synthesis and Application
of Two-Photon Active Fluorescent
Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging |
| title_full_unstemmed | Synthesis and Application
of Two-Photon Active Fluorescent
Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging |
| title_short | Synthesis and Application
of Two-Photon Active Fluorescent
Rhodol Dyes for Antibody Conjugation and In Vitro Cell Imaging |
| title_sort | synthesis and application
of two-photon active fluorescent
rhodol dyes for antibody conjugation and in vitro cell imaging |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308389/ https://www.ncbi.nlm.nih.gov/pubmed/37396252 http://dx.doi.org/10.1021/acsomega.3c01796 |
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