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Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media

[Image: see text] Rapid detection of nerve agents from complex matrices with minimal sample preparation is essential due to their high toxicity and bioavailability. In this work, quantum dots (QDs) were functionalized with oligonucleotide aptamers that specifically targeted a nerve agent metabolite,...

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Autores principales: Demers, Steven M. E., Kuhne, Wendy W., Swindle, Ashlee R., Dick, Don D., Coopersmith, Kaitlin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308513/
https://www.ncbi.nlm.nih.gov/pubmed/37396263
http://dx.doi.org/10.1021/acsomega.3c02173
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author Demers, Steven M. E.
Kuhne, Wendy W.
Swindle, Ashlee R.
Dick, Don D.
Coopersmith, Kaitlin J.
author_facet Demers, Steven M. E.
Kuhne, Wendy W.
Swindle, Ashlee R.
Dick, Don D.
Coopersmith, Kaitlin J.
author_sort Demers, Steven M. E.
collection PubMed
description [Image: see text] Rapid detection of nerve agents from complex matrices with minimal sample preparation is essential due to their high toxicity and bioavailability. In this work, quantum dots (QDs) were functionalized with oligonucleotide aptamers that specifically targeted a nerve agent metabolite, methylphosphonic acid (MePA). These QD-DNA bioconjugates were covalently linked to quencher molecules to form Förster resonance energy transfer (FRET) donor–acceptor pairs that quantitatively measure the presence of MePA. Using the FRET biosensor, the MePA limit of detection was 743 nM in artificial urine. A decrease in the QD lifetime was measured upon DNA binding and was recovered with MePA. The biosensor’s flexible design makes it a strong candidate for the rapid detection of chemical and biological agents for deployable, in-field detectors.
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spelling pubmed-103085132023-06-30 Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media Demers, Steven M. E. Kuhne, Wendy W. Swindle, Ashlee R. Dick, Don D. Coopersmith, Kaitlin J. ACS Omega [Image: see text] Rapid detection of nerve agents from complex matrices with minimal sample preparation is essential due to their high toxicity and bioavailability. In this work, quantum dots (QDs) were functionalized with oligonucleotide aptamers that specifically targeted a nerve agent metabolite, methylphosphonic acid (MePA). These QD-DNA bioconjugates were covalently linked to quencher molecules to form Förster resonance energy transfer (FRET) donor–acceptor pairs that quantitatively measure the presence of MePA. Using the FRET biosensor, the MePA limit of detection was 743 nM in artificial urine. A decrease in the QD lifetime was measured upon DNA binding and was recovered with MePA. The biosensor’s flexible design makes it a strong candidate for the rapid detection of chemical and biological agents for deployable, in-field detectors. American Chemical Society 2023-06-09 /pmc/articles/PMC10308513/ /pubmed/37396263 http://dx.doi.org/10.1021/acsomega.3c02173 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Demers, Steven M. E.
Kuhne, Wendy W.
Swindle, Ashlee R.
Dick, Don D.
Coopersmith, Kaitlin J.
Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media
title Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media
title_full Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media
title_fullStr Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media
title_full_unstemmed Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media
title_short Quantum Dot–DNA FRET Conjugates for Direct Analysis of Methylphosphonic Acid in Complex Media
title_sort quantum dot–dna fret conjugates for direct analysis of methylphosphonic acid in complex media
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308513/
https://www.ncbi.nlm.nih.gov/pubmed/37396263
http://dx.doi.org/10.1021/acsomega.3c02173
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