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Cytotoxic and Antioxidative Effects of Geranium Oil and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast Cancer Cells
[Image: see text] Geranium oil (GO) has antiproliferative, antiangiogenic, and anti-inflammatory properties. Ascorbic acid (AA) is reported to inhibit the formation of reactive oxygen species, sensitize cancer cells, and induce apoptosis. In this context, AA, GO, and AA-GO were loaded into niosomal...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308595/ https://www.ncbi.nlm.nih.gov/pubmed/37396262 http://dx.doi.org/10.1021/acsomega.3c01681 |
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author | Fahmy, Sherif Ashraf Nasr, Soad Ramzy, Asmaa Dawood, Abdelhameed S. Abdelnaser, Anwar Azzazy, Hassan Mohamed El-Said |
author_facet | Fahmy, Sherif Ashraf Nasr, Soad Ramzy, Asmaa Dawood, Abdelhameed S. Abdelnaser, Anwar Azzazy, Hassan Mohamed El-Said |
author_sort | Fahmy, Sherif Ashraf |
collection | PubMed |
description | [Image: see text] Geranium oil (GO) has antiproliferative, antiangiogenic, and anti-inflammatory properties. Ascorbic acid (AA) is reported to inhibit the formation of reactive oxygen species, sensitize cancer cells, and induce apoptosis. In this context, AA, GO, and AA-GO were loaded into niosomal nanovesicles to ameliorate the physicochemical properties of GO and improve its cytotoxic effects using the thin-film hydration technique. The prepared nanovesicles had a spherical shape with average diameters ranging from 200 to 300 nm and exhibited outstanding surface negative charges, high entrapment efficiencies, and a controlled sustained release over 72 h. Entrapping AA and GO in niosomes resulted in a lower IC(50) value than free AA and GO when tested on MCF-7 breast cancer cells. In addition, flow cytometry analysis showed higher apoptotic cells in the late apoptotic stage upon treating the MCF-7 breast cancer cells with AA-GO niosomal vesicles compared to treatments with free AA, free GO, and AA or GO loaded into niosomal nanovesicles. Assessing the antioxidant effect of the free drugs and loaded niosomal nanovesicles showed enhanced antioxidant activity of AA-GO niosomal vesicles. These findings suggest the AA-GO niosomal vesicles as a potential treatment strategy against breast cancer, possibly through scavenging free radicals. |
format | Online Article Text |
id | pubmed-10308595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-103085952023-06-30 Cytotoxic and Antioxidative Effects of Geranium Oil and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast Cancer Cells Fahmy, Sherif Ashraf Nasr, Soad Ramzy, Asmaa Dawood, Abdelhameed S. Abdelnaser, Anwar Azzazy, Hassan Mohamed El-Said ACS Omega [Image: see text] Geranium oil (GO) has antiproliferative, antiangiogenic, and anti-inflammatory properties. Ascorbic acid (AA) is reported to inhibit the formation of reactive oxygen species, sensitize cancer cells, and induce apoptosis. In this context, AA, GO, and AA-GO were loaded into niosomal nanovesicles to ameliorate the physicochemical properties of GO and improve its cytotoxic effects using the thin-film hydration technique. The prepared nanovesicles had a spherical shape with average diameters ranging from 200 to 300 nm and exhibited outstanding surface negative charges, high entrapment efficiencies, and a controlled sustained release over 72 h. Entrapping AA and GO in niosomes resulted in a lower IC(50) value than free AA and GO when tested on MCF-7 breast cancer cells. In addition, flow cytometry analysis showed higher apoptotic cells in the late apoptotic stage upon treating the MCF-7 breast cancer cells with AA-GO niosomal vesicles compared to treatments with free AA, free GO, and AA or GO loaded into niosomal nanovesicles. Assessing the antioxidant effect of the free drugs and loaded niosomal nanovesicles showed enhanced antioxidant activity of AA-GO niosomal vesicles. These findings suggest the AA-GO niosomal vesicles as a potential treatment strategy against breast cancer, possibly through scavenging free radicals. American Chemical Society 2023-06-13 /pmc/articles/PMC10308595/ /pubmed/37396262 http://dx.doi.org/10.1021/acsomega.3c01681 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Fahmy, Sherif Ashraf Nasr, Soad Ramzy, Asmaa Dawood, Abdelhameed S. Abdelnaser, Anwar Azzazy, Hassan Mohamed El-Said Cytotoxic and Antioxidative Effects of Geranium Oil and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast Cancer Cells |
title | Cytotoxic and Antioxidative
Effects of Geranium Oil
and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast
Cancer Cells |
title_full | Cytotoxic and Antioxidative
Effects of Geranium Oil
and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast
Cancer Cells |
title_fullStr | Cytotoxic and Antioxidative
Effects of Geranium Oil
and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast
Cancer Cells |
title_full_unstemmed | Cytotoxic and Antioxidative
Effects of Geranium Oil
and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast
Cancer Cells |
title_short | Cytotoxic and Antioxidative
Effects of Geranium Oil
and Ascorbic Acid Coloaded in Niosomes against MCF-7 Breast
Cancer Cells |
title_sort | cytotoxic and antioxidative
effects of geranium oil
and ascorbic acid coloaded in niosomes against mcf-7 breast
cancer cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308595/ https://www.ncbi.nlm.nih.gov/pubmed/37396262 http://dx.doi.org/10.1021/acsomega.3c01681 |
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