Efficient and versatile multiplex prime editing in hexaploid wheat

Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A substitution into reverse transcriptase in the ePPEmax* architecture. ePPEplus enhances the efficiency by an average 33.0-fold and 6.4...

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Detalles Bibliográficos
Autores principales: Ni, Pei, Zhao, Yidi, Zhou, Ximeng, Liu, Zehua, Huang, Zhengwei, Ni, Zhongfu, Sun, Qixin, Zong, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308706/
https://www.ncbi.nlm.nih.gov/pubmed/37386475
http://dx.doi.org/10.1186/s13059-023-02990-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308706/
https://www.ncbi.nlm.nih.gov/pubmed/37386475
http://dx.doi.org/10.1186/s13059-023-02990-1

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