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Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents

Aqueous two-phase systems (ATPSs) have long been recognized as versatile and efficient tools for the extraction of biomolecules, including amino acids. Recent advancements in the field have introduced a novel approach by utilizing deep eutectic solvents (DES) to form ATPs. This study aimed to determ...

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Autores principales: SÖĞÜTLÜ, İnci, Saeed, Shakir Mahmood, Adil, Mohaned, Yadav, Anupam, Abdulkareem Mahmood, Evan, Saadh, Mohamed J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308850/
https://www.ncbi.nlm.nih.gov/pubmed/37396827
http://dx.doi.org/10.1039/d3ra03092j
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author SÖĞÜTLÜ, İnci
Saeed, Shakir Mahmood
Adil, Mohaned
Yadav, Anupam
Abdulkareem Mahmood, Evan
Saadh, Mohamed J.
author_facet SÖĞÜTLÜ, İnci
Saeed, Shakir Mahmood
Adil, Mohaned
Yadav, Anupam
Abdulkareem Mahmood, Evan
Saadh, Mohamed J.
author_sort SÖĞÜTLÜ, İnci
collection PubMed
description Aqueous two-phase systems (ATPSs) have long been recognized as versatile and efficient tools for the extraction of biomolecules, including amino acids. Recent advancements in the field have introduced a novel approach by utilizing deep eutectic solvents (DES) to form ATPs. This study aimed to determine the phase diagrams for an ATPS made of polyethylene glycol dimethyl ether 250 and two types of NADESs, namely choline chloride as a hydrogen bond acceptor (HBA), and either sucrose or fructose as a hydrogen bond donor (HBD) with a molar ratio of 1 : 2. The measured tie-line results revealed that the hydrogen bonds of NADES may not be entirely disrupted in aqueous solutions, and thus, these ATPSs act as ternary-like systems. Additionally, the binodal data were fitted using two semi-empirical equations, namely Merchuk and Zafarani-Moattar et al. equations. Furthermore, the ATPSs mentioned above were applied to extract three amino acids, namely l-arginine, l-phenylalanine, and l-tyrosine, and demonstrated good extraction levels. Finally, the Diamond–Hsu equation and its modified version were utilized to correlate the experimental partition coefficients of the amino acids. These advancements pave the way for the development of improved extraction methodologies and the exploration of new applications in the field of biotechnology, pharmaceuticals, and beyond.
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spelling pubmed-103088502023-06-30 Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents SÖĞÜTLÜ, İnci Saeed, Shakir Mahmood Adil, Mohaned Yadav, Anupam Abdulkareem Mahmood, Evan Saadh, Mohamed J. RSC Adv Chemistry Aqueous two-phase systems (ATPSs) have long been recognized as versatile and efficient tools for the extraction of biomolecules, including amino acids. Recent advancements in the field have introduced a novel approach by utilizing deep eutectic solvents (DES) to form ATPs. This study aimed to determine the phase diagrams for an ATPS made of polyethylene glycol dimethyl ether 250 and two types of NADESs, namely choline chloride as a hydrogen bond acceptor (HBA), and either sucrose or fructose as a hydrogen bond donor (HBD) with a molar ratio of 1 : 2. The measured tie-line results revealed that the hydrogen bonds of NADES may not be entirely disrupted in aqueous solutions, and thus, these ATPSs act as ternary-like systems. Additionally, the binodal data were fitted using two semi-empirical equations, namely Merchuk and Zafarani-Moattar et al. equations. Furthermore, the ATPSs mentioned above were applied to extract three amino acids, namely l-arginine, l-phenylalanine, and l-tyrosine, and demonstrated good extraction levels. Finally, the Diamond–Hsu equation and its modified version were utilized to correlate the experimental partition coefficients of the amino acids. These advancements pave the way for the development of improved extraction methodologies and the exploration of new applications in the field of biotechnology, pharmaceuticals, and beyond. The Royal Society of Chemistry 2023-06-29 /pmc/articles/PMC10308850/ /pubmed/37396827 http://dx.doi.org/10.1039/d3ra03092j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
SÖĞÜTLÜ, İnci
Saeed, Shakir Mahmood
Adil, Mohaned
Yadav, Anupam
Abdulkareem Mahmood, Evan
Saadh, Mohamed J.
Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
title Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
title_full Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
title_fullStr Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
title_full_unstemmed Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
title_short Extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
title_sort extraction of some essential amino acids using aqueous two-phase systems made by sugar-based deep eutectic solvents
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10308850/
https://www.ncbi.nlm.nih.gov/pubmed/37396827
http://dx.doi.org/10.1039/d3ra03092j
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