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Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis

Objective  Recent observations support the hypothesis that an imbalance between angiogenic factors has a fundamental role in the pathogenesis of pre-eclampsia and is responsible for the clinical manifestations of the disease. The goal of the present study was to evaluate the sensitivity, specificity...

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Autores principales: Sá, Catherine Primo Nogueira de, Jiménez, Mirela Foresti, Rosa, Marcos Wengrover, Arlindo, Ellen Machado, Ayub, Antonio Celso Koehler, Cardoso, Rodrigo Bernardes, Kreitchmann, Régis, El Beitune, Patrícia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Revinter Publicações Ltda. 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309227/
https://www.ncbi.nlm.nih.gov/pubmed/33254263
http://dx.doi.org/10.1055/s-0040-1713916
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author Sá, Catherine Primo Nogueira de
Jiménez, Mirela Foresti
Rosa, Marcos Wengrover
Arlindo, Ellen Machado
Ayub, Antonio Celso Koehler
Cardoso, Rodrigo Bernardes
Kreitchmann, Régis
El Beitune, Patrícia
author_facet Sá, Catherine Primo Nogueira de
Jiménez, Mirela Foresti
Rosa, Marcos Wengrover
Arlindo, Ellen Machado
Ayub, Antonio Celso Koehler
Cardoso, Rodrigo Bernardes
Kreitchmann, Régis
El Beitune, Patrícia
author_sort Sá, Catherine Primo Nogueira de
collection PubMed
description Objective  Recent observations support the hypothesis that an imbalance between angiogenic factors has a fundamental role in the pathogenesis of pre-eclampsia and is responsible for the clinical manifestations of the disease. The goal of the present study was to evaluate the sensitivity, specificity, and the best accuracy level of Soluble fms-like tyrosine kinase-1 (sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio in maternal serum and protein/creatinine ratio in urine sample to define the best cutoff point of these tests to discriminate between the patients with gestational hypertension and the patients with pre-eclampsia, to evaluate the possibility of using them as diagnostic methods. Methods  A prospective longitudinal study was performed, and blood samples were collected from 95 pregnant patients with hypertension to measure serum concentrations of biomarkers sFlt-1 and PlGF. Urine samples were collected for protein screening. Significance was set as p  < 0.05. Results  The sFlt-1/PlGF ratio demonstrated a sensitivity of 57.5% and a specificity of 60% using 50.4 as a cutoff point. The test that showed the best accuracy in the diagnosis of pre-eclampsia was protein/creatinine ratio, with a sensitivity of 78.9% and a specificity of 70% using 0.4 as a cutoff point and showing an area under the receiver operating characteristic curve of 0.80 ( p  < 0.001). Conclusion  No studied laboratory test proved to be fairly accurate for the diagnosis of pre-eclampsia, except for the protein/creatinine ratio. The evidence is insufficient to recommend biomarkers sFlt-1 and PlGF to be used for the diagnosis of pre-eclampsia.
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spelling pubmed-103092272023-07-27 Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis Sá, Catherine Primo Nogueira de Jiménez, Mirela Foresti Rosa, Marcos Wengrover Arlindo, Ellen Machado Ayub, Antonio Celso Koehler Cardoso, Rodrigo Bernardes Kreitchmann, Régis El Beitune, Patrícia Rev Bras Ginecol Obstet Objective  Recent observations support the hypothesis that an imbalance between angiogenic factors has a fundamental role in the pathogenesis of pre-eclampsia and is responsible for the clinical manifestations of the disease. The goal of the present study was to evaluate the sensitivity, specificity, and the best accuracy level of Soluble fms-like tyrosine kinase-1 (sFlt-1), placental growth factor (PlGF), and sFlt-1/PlGF ratio in maternal serum and protein/creatinine ratio in urine sample to define the best cutoff point of these tests to discriminate between the patients with gestational hypertension and the patients with pre-eclampsia, to evaluate the possibility of using them as diagnostic methods. Methods  A prospective longitudinal study was performed, and blood samples were collected from 95 pregnant patients with hypertension to measure serum concentrations of biomarkers sFlt-1 and PlGF. Urine samples were collected for protein screening. Significance was set as p  < 0.05. Results  The sFlt-1/PlGF ratio demonstrated a sensitivity of 57.5% and a specificity of 60% using 50.4 as a cutoff point. The test that showed the best accuracy in the diagnosis of pre-eclampsia was protein/creatinine ratio, with a sensitivity of 78.9% and a specificity of 70% using 0.4 as a cutoff point and showing an area under the receiver operating characteristic curve of 0.80 ( p  < 0.001). Conclusion  No studied laboratory test proved to be fairly accurate for the diagnosis of pre-eclampsia, except for the protein/creatinine ratio. The evidence is insufficient to recommend biomarkers sFlt-1 and PlGF to be used for the diagnosis of pre-eclampsia. Thieme Revinter Publicações Ltda. 2020-11-30 2020-11 /pmc/articles/PMC10309227/ /pubmed/33254263 http://dx.doi.org/10.1055/s-0040-1713916 Text en The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. ( https://creativecommons.org/licenses/by/4.0/ ) https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Sá, Catherine Primo Nogueira de
Jiménez, Mirela Foresti
Rosa, Marcos Wengrover
Arlindo, Ellen Machado
Ayub, Antonio Celso Koehler
Cardoso, Rodrigo Bernardes
Kreitchmann, Régis
El Beitune, Patrícia
Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis
title Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis
title_full Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis
title_fullStr Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis
title_full_unstemmed Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis
title_short Evaluation of Angiogenic Factors (PlGF and sFlt-1) in Pre-eclampsia Diagnosis
title_sort evaluation of angiogenic factors (plgf and sflt-1) in pre-eclampsia diagnosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309227/
https://www.ncbi.nlm.nih.gov/pubmed/33254263
http://dx.doi.org/10.1055/s-0040-1713916
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