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The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis

Purpose To evaluate the effect of mesenchymal stem cells (MSCs) on fertility in experimental retrocervical endometriosis. Methods A total of 27 New Zealand rabbits were divided into three groups: endometriosis, in which endometrial implants were created; mesenchymal, in which MSCs were applied in ad...

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Autores principales: Abreu, Jaqueline Pedroso de, Rebelatto, Carmen Lúcia Kuniyoshi, Savari, Carolina Antunes, Capriglione, Luiz Guilherme Achcar, Miyague, Lye, Noronha, Lucia de, Amaral, Vivian Ferreira do
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Thieme Revinter Publicações Ltda 2017
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309402/
https://www.ncbi.nlm.nih.gov/pubmed/28399593
http://dx.doi.org/10.1055/s-0037-1601484
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author Abreu, Jaqueline Pedroso de
Rebelatto, Carmen Lúcia Kuniyoshi
Savari, Carolina Antunes
Capriglione, Luiz Guilherme Achcar
Miyague, Lye
Noronha, Lucia de
Amaral, Vivian Ferreira do
author_facet Abreu, Jaqueline Pedroso de
Rebelatto, Carmen Lúcia Kuniyoshi
Savari, Carolina Antunes
Capriglione, Luiz Guilherme Achcar
Miyague, Lye
Noronha, Lucia de
Amaral, Vivian Ferreira do
author_sort Abreu, Jaqueline Pedroso de
collection PubMed
description Purpose To evaluate the effect of mesenchymal stem cells (MSCs) on fertility in experimental retrocervical endometriosis. Methods A total of 27 New Zealand rabbits were divided into three groups: endometriosis, in which endometrial implants were created; mesenchymal, in which MSCs were applied in addition to the creation of endometrial implants; and control, the group without endometriosis. Fisher's exact test was performed to compare the dichotomous qualitative variables among the groups. The quantitative variables were compared by the nonparametric Mann-Whitney and Kruskal-Wallis tests. The Mann-Whitney test was used for post-hoc multiple comparison with Boniferroni correction. Results Regarding the beginning of the fertile period, the three groups had medians of 14 ± 12.7, 40 ± 5, and 33 ± 8.9 days respectively (p = 0.005). With regard to fertility (number of pregnancies), the endometriosis and control groups showed a rate of 77.78%, whereas the mesenchymal group showed a rate of 11.20% (p = 0.015). No differences in Keenan's histological classification were observed among the groups (p = 0.730). With regard to the macroscopic appearance of the lesions, the mesenchymal group showed the most pelvic adhesions. Conclusion The use of MSCs in endometriosis negatively contributed to fertility, suggesting the role of these cells in the development of this disease.
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spelling pubmed-103094022023-07-27 The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis Abreu, Jaqueline Pedroso de Rebelatto, Carmen Lúcia Kuniyoshi Savari, Carolina Antunes Capriglione, Luiz Guilherme Achcar Miyague, Lye Noronha, Lucia de Amaral, Vivian Ferreira do Rev Bras Ginecol Obstet Purpose To evaluate the effect of mesenchymal stem cells (MSCs) on fertility in experimental retrocervical endometriosis. Methods A total of 27 New Zealand rabbits were divided into three groups: endometriosis, in which endometrial implants were created; mesenchymal, in which MSCs were applied in addition to the creation of endometrial implants; and control, the group without endometriosis. Fisher's exact test was performed to compare the dichotomous qualitative variables among the groups. The quantitative variables were compared by the nonparametric Mann-Whitney and Kruskal-Wallis tests. The Mann-Whitney test was used for post-hoc multiple comparison with Boniferroni correction. Results Regarding the beginning of the fertile period, the three groups had medians of 14 ± 12.7, 40 ± 5, and 33 ± 8.9 days respectively (p = 0.005). With regard to fertility (number of pregnancies), the endometriosis and control groups showed a rate of 77.78%, whereas the mesenchymal group showed a rate of 11.20% (p = 0.015). No differences in Keenan's histological classification were observed among the groups (p = 0.730). With regard to the macroscopic appearance of the lesions, the mesenchymal group showed the most pelvic adhesions. Conclusion The use of MSCs in endometriosis negatively contributed to fertility, suggesting the role of these cells in the development of this disease. Thieme Revinter Publicações Ltda 2017-04-11 2017-05 /pmc/articles/PMC10309402/ /pubmed/28399593 http://dx.doi.org/10.1055/s-0037-1601484 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.
spellingShingle Abreu, Jaqueline Pedroso de
Rebelatto, Carmen Lúcia Kuniyoshi
Savari, Carolina Antunes
Capriglione, Luiz Guilherme Achcar
Miyague, Lye
Noronha, Lucia de
Amaral, Vivian Ferreira do
The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis
title The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis
title_full The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis
title_fullStr The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis
title_full_unstemmed The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis
title_short The Effect of Mesenchymal Stem Cells on Fertility in Experimental Retrocervical Endometriosis
title_sort effect of mesenchymal stem cells on fertility in experimental retrocervical endometriosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10309402/
https://www.ncbi.nlm.nih.gov/pubmed/28399593
http://dx.doi.org/10.1055/s-0037-1601484
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